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G6PD recombinant protein

Scan QR to view Datasheet Catalog #    MBS844280 G6PD recombinant protein
Unit / Price
0.1 mg  /  $375 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

G6PD, Recombinant Protein

 Also Known As   

G6PD, Human Recombinant

 Product Synonym Names    Glucose-6-phosphate 1-dehydrogenase; Zwf.
 Product Gene Name   

G6PD recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL
 OMIM    300908
 3D Structure    ModBase 3D Structure for P11413
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 Host    Hi-5 cells, Baculovirus system
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 Purity/Purification    >=95%
 Form/Format    0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 mM PMSF, 2 mM EDTA, 2 mM DTT, 200 mM NaCl.
Appearance: Liquid
 Concentration    0.5 mg/ml (lot specific)
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 Biological Activity    Specific activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-Dgluconate per min in the presence of beta-NADP at pH 7.4 at 25 degree C.
 Results    Specific activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-Dgluconate per min in the presence of beta-NADP at pH 7.4 at 25 degree C.
 Handling    Centrifuge the vial prior to opening.
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 Preparation and Storage    At -20 degree C
Shelf Life: 6 months
 Other Notes    Small volumes of G6PD recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for G6PD recombinant protein

   Background: Glucose-6-phosphate dehydrogenase is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant human G6PD protein, fused to His-tag at N-terminus, was expressed in Hi-5 cells using baculovirus expression system and purified by using conventional chromatography.
 Product Categories/Family for G6PD recombinant protein    Proteins and Enzymes; Proteins and Enzymes (A-Z)Diabetes, Obesity & Metabolic Syndrome; Signaling Pathways in Diabetes and Obesity; AMPK, Insulin and Leptin Signaling; Proteins/Enzymes
 Applications Tested/Suitable for G6PD recombinant protein   

SDS-PAGE

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NCBI/Uniprot data below describe general gene information for G6PD. It may not necessarily be applicable to this product.
 NCBI GI #    109389365
 NCBI GeneID    2539
 NCBI Accession #    NP_000393.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_000402.4 [Other Products]
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 UniProt Primary Accession #    P11413 [Other Products]
 UniProt Secondary Accession #    Q16000; Q16765; Q8IU70; Q8IU88; Q8IUA6; Q96PQ2; D3DWX9 [Other Products]
 UniProt Related Accession #    P11413 [Other Products]
 Molecular Weight    61.4 kDa (515 aa, 1-515 aa)
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 NCBI Official Full Name    glucose-6-phosphate 1-dehydrogenase isoform a
 NCBI Official Synonym Full Names    glucose-6-phosphate dehydrogenase
 NCBI Official Symbol    G6PD [Similar Products]
 NCBI Official Synonym Symbols   
G6PD1
[Similar Products]
 NCBI Protein Information    glucose-6-phosphate 1-dehydrogenase
 UniProt Protein Name    Glucose-6-phosphate 1-dehydrogenase
 UniProt Gene Name    G6PD [Similar Products]
 UniProt Synonym Gene Names    G6PD [Similar Products]
 UniProt Entry Name    G6PD_HUMAN
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 NCBI Summary for G6PD    This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for G6PD    G6PD: glucose-6-phosphate dehydrogenase. A cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two splice variant isoforms have been described. The long isoform is found in lymphoblasts, granulocytes and sperm.

Protein type: EC 1.1.1.49; Cell development/differentiation; Oxidoreductase; Carbohydrate Metabolism - pentose phosphate pathway; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: centrosome; internal side of plasma membrane; intracellular membrane-bound organelle; membrane; cytoplasm; microtubule organizing center; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; glucose-6-phosphate dehydrogenase activity; protein homodimerization activity; NADP binding; glucose binding

Biological Process: NADPH regeneration; response to food; pentose-phosphate shunt; erythrocyte maturation; ribose phosphate biosynthetic process; glucose 6-phosphate metabolic process; pentose-phosphate shunt, oxidative branch; cytokine production; glucose metabolic process; pathogenesis; cholesterol biosynthetic process; NADP metabolic process; response to organic cyclic substance; glutathione metabolic process; response to ethanol; substantia nigra development; carbohydrate metabolic process; regulation of neuron apoptosis; lipid metabolic process; pentose biosynthetic process

Disease: Favism, Susceptibility To; Anemia, Nonspherocytic Hemolytic, Due To G6pd Deficiency; Malaria, Susceptibility To
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 Research Articles on G6PD    1. These results show that the location of the mutation affects the catalytic properties, stability and structure of G6PD and that these changes are closely associated with the clinical presentation of its deficiency.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with G6PD recombinant proteinDiseases associated with G6PD recombinant protein
 Reference Product  PubMed Publications
 GPI recombinant protein  >59 publications with G6PD and GPI
 PGD recombinant protein  >58 publications with G6PD and PGD
 PGM1 recombinant protein  >23 publications with G6PD and PGM1
 LDHB recombinant protein  >14 publications with G6PD and LDHB
 H6PD recombinant protein  >4 publications with G6PD and H6PD
 UBC recombinant protein  >2 publications with G6PD and UBC
 HK1 recombinant protein  >1 publications with G6PD and HK1
 TALDO1 recombinant protein  >1 publications with G6PD and TALDO1
 Disease Name  Pubmed Publications
 Glucosephosphate Dehydrogenase Deficiency Antibodies  >5048 publications with G6PD and Glucosephosphate Dehydrogenase Deficiency
 Anemia Antibodies  >4805 publications with G6PD and Anemia
 Anemia, Hemolytic Antibodies  >4701 publications with G6PD and Anemia, Hemolytic
 Hemolysis Antibodies  >974 publications with G6PD and Hemolysis
 Poisoning Antibodies  >666 publications with G6PD and Poisoning
 Favism Antibodies  >596 publications with G6PD and Favism
 Nervous System Diseases Antibodies  >376 publications with G6PD and Nervous System Diseases
 Liver Diseases Antibodies  >247 publications with G6PD and Liver Diseases
 Anemia, Hemolytic, Congenital Nonspherocytic Antibodies  >214 publications with G6PD and Anemia, Hemolytic, Congenital Nonspherocytic
 Brain Diseases Antibodies  >199 publications with G6PD and Brain Diseases
Organs/Tissues associated with G6PD recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3650 publications with G6PD and Blood
 Liver Antibodies  >556 publications with G6PD and Liver
 Kidney Antibodies  >209 publications with G6PD and Kidney
 Muscle Antibodies  >144 publications with G6PD and Muscle
 Bone Antibodies  >144 publications with G6PD and Bone
 Brain Antibodies  >132 publications with G6PD and Brain
 Skin Antibodies  >115 publications with G6PD and Skin
 Bone Marrow Antibodies  >101 publications with G6PD and Bone Marrow
 Lung Antibodies  >86 publications with G6PD and Lung
 Vascular Antibodies  >79 publications with G6PD and Vascular
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