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GLuD1 recombinant protein

Scan QR to view Datasheet Catalog #    MBS205535
SDS-PAGE
Unit / Price
0.1 mg  /  $310 +1 FREE 8GB USB
0.5 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GLuD1, Recombinant Protein

 Also Known As   

GLuD1, 54-558aa, Human, His tag, E Coli

 Product Synonym Names    Glutamate dehydrogenase 1; mitochondrial; GDH; GDH1; GLuD
 Product Gene Name   

GLuD1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MGSSEAVADR EDDPNFFKMV EGFFDRGASI VEDKLVEDLR TRESEEQKRN RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG DKTFVVQGFG NVGLHSMRYL HRFGAKCIAV GESDGSIWNP DGIDPKELED FKLQHGSILG FPKAKPYEGS ILEADCDILI PAASEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER NIMVIPDLYL NAGGVTVSYF EWLKNLNHVS YGRLTFKYER DSNYHLLMSV QESLERKFGK HGGTIPIVPT AEFQDRISGA SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV NAIEKVFKVY NEAGVTFT
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 Purity/Purification    > 80% by SDS - PAGE
 Form/Format    Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
 Concentration    1mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag    His-tag
 Expression System    E Coli
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of GLuD1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for GLuD1 recombinant protein

   Glutamate dehydrogenase 1, mitochondrial precursor, also known as GLuD1, belongs to the Glu/Leu/Phe/Val dehydrogenases family. GLuD1 has a central role in nitrogen metabolism in plants and animals. GLuD1 is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Mutations in the gene encoding GLuD1 causes hyperinsulinism-hyperammonemia syndrome (HHS), which is an inherited condition characterized by high insulin and ammonia levels in the blood. GLuD1 may also be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Recombinant human GLuD1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
 Product Categories/Family for GLuD1 recombinant protein    Metabolism
 Applications Tested/Suitable for GLuD1 recombinant protein   

SDS-PAGE

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 SDS-PAGE of GLuD1 recombinant protein    GLuD1 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for GLuD1. It may not necessarily be applicable to this product.
 NCBI GI #    4885281
 NCBI GeneID    2746
 NCBI Accession #    NP_005262 [Other Products]
 NCBI GenBank Nucleotide #    NM_005271.3 [Other Products]
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 UniProt Secondary Accession #    Q5TBU3; B3KV55; B4DGN5 [Other Products]
 UniProt Related Accession #    P00367 [Other Products]
 Molecular Weight    58.4 kDa (528aa)
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 NCBI Official Full Name    glutamate dehydrogenase 1, mitochondrial
 NCBI Official Synonym Full Names    glutamate dehydrogenase 1
 NCBI Official Symbol    GLUD1 [Similar Products]
 NCBI Official Synonym Symbols   
GDH; GDH1; GLUD
[Similar Products]
 NCBI Protein Information    glutamate dehydrogenase 1, mitochondrial; GDH 1; glutamate dehydrogenase (NAD(P)+)
 UniProt Protein Name    Glutamate dehydrogenase 1, mitochondrial
 UniProt Gene Name    GLUD1 [Similar Products]
 UniProt Synonym Gene Names    GLUD; GDH 1 [Similar Products]
 UniProt Entry Name    DHE3_HUMAN
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 NCBI Summary for GLuD1    This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans.[provided by RefSeq, Sep 2009]
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 UniProt Comments for GLuD1    GLUD1: May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Homohexamer. Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP. ADP can occupy the NADH binding site and activate the enzyme. Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Protein type: EC 1.4.1.3; Mitochondrial; Amino Acid Metabolism - arginine and proline; Energy Metabolism - nitrogen; Oxidoreductase; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm

Molecular Function: identical protein binding; protein binding; glutamate dehydrogenase activity; GTP binding; glutamate dehydrogenase [NAD(P)+] activity; ADP binding; ATP binding

Biological Process: glutamate catabolic process; substantia nigra development; positive regulation of insulin secretion; glutamine metabolic process; amino acid biosynthetic process; glutamate biosynthetic process

Disease: Hyperinsulinemic Hypoglycemia, Familial, 6
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Product References and Citations for GLuD1 recombinant protein

   Mastorodemos V., et al. (2009) Biochem Cell Biol. 87:505-516.
Tanizawa Y., et al. (2002) Diabetes. 51:712-717.
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 Research Articles on GLuD1    1. The GDH1 is a key metabolic enzyme with emerging roles in insulin regulation. MitoNEET forms a covalent complex with GDH1 through disulfide bond formation and acts as an activator.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GLuD1 recombinant proteinPathways associated with GLuD1 recombinant protein
 Reference Product  PubMed Publications
 GLUL recombinant protein  >2 publications with GLuD1 and GLUL
 Products by Pathway  Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 D-Glutamine And D-glutamate Metabolism Pathway antibodies  D-Glutamine And D-glutamate Metabolism Pathway Diagram
 D-Glutamine And D-glutamate Metabolism Pathway antibodies  D-Glutamine And D-glutamate Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
Diseases associated with GLuD1 recombinant proteinOrgans/Tissues associated with GLuD1 recombinant protein
 Disease Name  Pubmed Publications
 Hyperinsulinism Antibodies  >58 publications with GLuD1 and Hyperinsulinism
 Hyperammonemia Antibodies  >28 publications with GLuD1 and Hyperammonemia
 Nervous System Diseases Antibodies  >14 publications with GLuD1 and Nervous System Diseases
 Seizures Antibodies  >10 publications with GLuD1 and Seizures
 Hyperplasia Antibodies  >5 publications with GLuD1 and Hyperplasia
 Hyperinsulinemic hypoglycemia, familial, 6 Antibodies  >5 publications with GLuD1 and Hyperinsulinemic hypoglycemia, familial, 6
 Movement Disorders Antibodies  >4 publications with GLuD1 and Movement Disorders
 Inflammation Antibodies  >3 publications with GLuD1 and Inflammation
 Hyperglycemia Antibodies  >2 publications with GLuD1 and Hyperglycemia
 Disease Models, Animal Antibodies  >2 publications with GLuD1 and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >47 publications with GLuD1 and Brain
 Pancreas Antibodies  >20 publications with GLuD1 and Pancreas
 Blood Antibodies  >17 publications with GLuD1 and Blood
 Nerve Antibodies  >14 publications with GLuD1 and Nerve
 Liver Antibodies  >10 publications with GLuD1 and Liver
 Testis Antibodies  >7 publications with GLuD1 and Testis
 Kidney Antibodies  >4 publications with GLuD1 and Kidney
 Placenta Antibodies  >2 publications with GLuD1 and Placenta
 Intestine Antibodies  >1 publications with GLuD1 and Intestine
 Eye Antibodies  >1 publications with GLuD1 and Eye
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