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GALC recombinant protein :: Galactosylceramidase (GALC) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2009657 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $160 +1 FREE 8GB USB
0.05 mg  /  $315 +1 FREE 8GB USB
0.1 mg  /  $490 +1 FREE 8GB USB
0.2 mg  /  $600 +1 FREE 8GB USB
0.5 mg  /  $1,170 +1 FREE 8GB USB
1 mg  /  $1,745 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Galactosylceramidase (GALC), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Galactosylceramidase (GALC)

 Product Gene Name   

GALC recombinant protein

[Similar Products]
 Matching Pairs    Antibody: Galactosylceramidase (GALC) (MBS2004871)
Antigen: Galactosylceramidase (GALC) (MBS2009657)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The sequence of the target protein is listed below.
DGLGNL TIIIETMSHK HSKCIRPFLP YFNVSQQFAT FVLKGSFSEI PELQVWYTKL GKTSERFLFK QLDSLWLLDS DGSFTLSLHE DELFTLTTLT TGRKGSYPLP PKSQPFPSTY KDDFNVDYPF FSEAPNFADQ TGVFEYFTNI EDPGEHHFTL RQVLN
 Chromosome Location    Chromosome: 14; NC_000014.8 (88304164..88460009, complement). Location: 14q31
 OMIM    245200
 3D Structure    ModBase 3D Structure for P54803
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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 Organism    Homo sapiens (Human)
 Expression System    Prokaryotic expression
 Residues    Asp375~Asn535 (Accession # P54803) with N-terminal His-Tag
 Predicted isoelectric point    5.7
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of GALC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for GALC recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for GALC recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
May be suitable for use in other assays to be determined by the end user.

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 SDS-PAGE of GALC recombinant protein    GALC recombinant protein SDS-PAGE image
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 SDS-PAGE of GALC recombinant protein    [ DESCRIPTION]
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa.
Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SDS-polyacrylamide gel.
[ LOADING VOLUMES]
3~5uL per well for mini gel; 7uL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
GALC recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for GALC. It may not necessarily be applicable to this product.
 NCBI GI #    83281450
 NCBI GeneID    2581
 NCBI Accession #    NP_000144.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000153.3 [Other Products]
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 UniProt Primary Accession #    P54803 [Other Products]
 UniProt Secondary Accession #    Q8J030; B4DKE8; B4DYN1; B4DZJ8; B7Z7Z2; J3KN25; J3KPP8 [Other Products]
 UniProt Related Accession #    P54803 [Other Products]
 Molecular Weight    20.1kDa
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 NCBI Official Full Name    galactocerebrosidase isoform a
 NCBI Official Synonym Full Names    galactosylceramidase
 NCBI Official Symbol    GALC [Similar Products]
 NCBI Protein Information    galactocerebrosidase; GALCERase; galactosylceraminidase; galactocerebroside beta-galactosidase; galactosylceramide beta-galactosidase
 UniProt Protein Name    Galactocerebrosidase
 UniProt Synonym Protein Names   
Galactocerebroside beta-galactosidase; Galactosylceramidase; Galactosylceramide beta-galactosidase
 Protein Family    Galactocerebrosidase
 UniProt Gene Name    GALC [Similar Products]
 UniProt Synonym Gene Names    GALCERase [Similar Products]
 UniProt Entry Name    GALC_HUMAN
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 NCBI Summary for GALC    This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GALC    GALC: Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. Defects in GALC are the cause of leukodystrophy globoid cell (GLD); also known as Krabbe disease. This autosomal recessive disorder results in the insufficient catabolism of several galactolipids that are important in the production of normal myelin. Clinically, the most frequent form is the infantile form. Most patients (90%) present before six months of age with irritability, spasticity, arrest of motor and mental development, and bouts of temperature elevation without infection. This is followed by myoclonic jerks of arms and legs, oposthotonus, hypertonic fits, and mental regression, which progresses to a severe decerebrate condition with no voluntary movements and death from respiratory infections or cerebral hyperpyrexia before 2 years of age. However, a significant number of cases with later onset, presenting with unexplained blindness, weakness and/or progressive motor, and sensory neuropathy that can progress to severe mental incapacity and death, have been identified. Belongs to the glycosyl hydrolase 59 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.1.46; Hydrolase; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 14q31

Cellular Component: lysosomal lumen; lysosome

Molecular Function: galactosylceramidase activity

Biological Process: sphingolipid metabolic process; carbohydrate metabolic process; glycosphingolipid metabolic process; galactosylceramide catabolic process

Disease: Krabbe Disease
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 Research Articles on GALC    1. Higher galactocerebrosidase activity is predictive of later symptom onset times but does not predict survival after symptom onset when controlling for the logarithm of age at onset.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GALC recombinant proteinPathways associated with GALC recombinant protein
 Reference Product  PubMed Publications
 GLA recombinant protein  >3 publications with GALC and GLA
 UGCG recombinant protein  >2 publications with GALC and UGCG
 SMPD1 recombinant protein  >2 publications with GALC and SMPD1
 ARSA recombinant protein  >2 publications with GALC and ARSA
 ASAH1 recombinant protein  >2 publications with GALC and ASAH1
 SGPP1 recombinant protein  >1 publications with GALC and SGPP1
 GAL3ST1 recombinant protein  >1 publications with GALC and GAL3ST1
 GBA recombinant protein  >1 publications with GALC and GBA
 Products by Pathway  Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
Diseases associated with GALC recombinant proteinOrgans/Tissues associated with GALC recombinant protein
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >157 publications with GALC and Nervous System Diseases
 Leukodystrophy, Globoid Cell Antibodies  >110 publications with GALC and Leukodystrophy, Globoid Cell
 Neoplasms Antibodies  >15 publications with GALC and Neoplasms
 Inflammation Antibodies  >15 publications with GALC and Inflammation
 Glioma Antibodies  >10 publications with GALC and Glioma
 Encephalomyelitis, Autoimmune, Experimental Antibodies  >6 publications with GALC and Encephalomyelitis, Autoimmune, Experimental
 Seizures Antibodies  >6 publications with GALC and Seizures
 Cardiovascular Diseases Antibodies  >6 publications with GALC and Cardiovascular Diseases
 Nervous System Malformations Antibodies  >2 publications with GALC and Nervous System Malformations
 Edema Antibodies  >2 publications with GALC and Edema
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >191 publications with GALC and Brain
 Blood Antibodies  >59 publications with GALC and Blood
 Bone Antibodies  >34 publications with GALC and Bone
 Bone Marrow Antibodies  >30 publications with GALC and Bone Marrow
 Embryonic Tissue Antibodies  >23 publications with GALC and Embryonic Tissue
 Muscle Antibodies  >11 publications with GALC and Muscle
 Skin Antibodies  >8 publications with GALC and Skin
 Kidney Antibodies  >6 publications with GALC and Kidney
 Connective Tissue Antibodies  >5 publications with GALC and Connective Tissue
 Amniotic Fluid Antibodies  >5 publications with GALC and Amniotic Fluid
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