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LGALS3 recombinant protein :: Galectin-3 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS203603
SDS-PAGE
Unit / Price
0.1 mg  /  $285 +1 FREE 8GB USB
0.5 mg  /  $640 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Galectin-3 (LGALS3), Recombinant Protein

 Also Known As   

Galectin-3, 1-250aa, Human, His-tag, E Coli

 Product Synonym Names    CBP35; GAL3; GALBP; GALIG; LGALS2; MAC2; 35 kDa lectin; Carbohydrate binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Galectin 3 internal gene; included; Galectin-3; Galectin3; GBP; IgE binding protein; IgE-binding protein; L 31; L 34; L-34 galactoside-binding lectin; L31; LGALS3; Laminin-binding protein; Lectin L-29; MAC 2 antigen; Lectin; galactose binding; soluble 3; Mac-2; Macrophage galactose-specific lectin; MGC105387.
 Product Gene Name   

LGALS3 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI
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 Purity/Purification    > 95% by SDS-PAGE
 Form/Format    20 mM Tris-HCl buffer,(pH8.0), 10% Glycerol, 1mM DTT, 0.1M NaCl
 Concentration    0.5mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag    His-tag
 Expression System    E Coli
 Bioactivity    The ED50 for this effect is 10-20 ug/ml. Measured by its ability to agglutinate human red blood cells.
 Assay    1. Mix equal volumes of human blood and Alsever's solution (pH 7.0).
(Alsever's solution: NaCl 0.42 g, Sodium citric acid 0.8g, Citric acid 0.055 g, D-glucose 2.05g in DW100 ml)
2. Centrifuge at 15000rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin-EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37C and wash four times with PBS.
5. Dilute packed cells in PBS to give 4% red cell suspension.
6. Load 50ul of 0.5%BSA-in-0.15M-NaCl solution and 25ul of 4%-Red-Cell-in-PBS in u shaped wells.
7. Add 25ul of serial diluted galectin protein in PBS to each well plate. (Round bottom 96 well plate)
8. Incubate for 30min at room temperature to observe visible agglutination.
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of LGALS3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for LGALS3 recombinant protein

   Galectin-3 is a member of the family of animal lectins, which selectively binds beta-galactoside residues. This protein is secreted from cells by ectocytosis, which is independent of the classical secretory pathway through the endoplasmic reticulum/Golgi network. Galectin-3 has been associated with the inhibition of apoptosis and the progression of cancer. It is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. Recombinant Galectin-3 protein was expressed in E.coli and purified by using conventional chromatography techniques.
 Product Categories/Family for LGALS3 recombinant protein    Differentiation & Regeneration
 Applications Tested/Suitable for LGALS3 recombinant protein   

SDS-PAGE

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 SDS-PAGE of LGALS3 recombinant protein    LGALS3 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for LGALS3. It may not necessarily be applicable to this product.
 NCBI GI #    115430223
 NCBI GeneID    3958
 NCBI Accession #    NP_002297 [Other Products]
 NCBI GenBank Nucleotide #    NM_002306.3 [Other Products]
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 UniProt Secondary Accession #    Q16005; Q6IBA7; Q96J47; B2RC38 [Other Products]
 UniProt Related Accession #    P17931 [Other Products]
 Molecular Weight    28.3 kDa (270aa), confirmed by MALDI-TOF. [Similar Products]
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 NCBI Official Full Name    galectin-3 isoform 1
 NCBI Official Synonym Full Names    lectin, galactoside-binding, soluble, 3
 NCBI Official Symbol    LGALS3 [Similar Products]
 NCBI Official Synonym Symbols   
L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
[Similar Products]
 NCBI Protein Information    galectin-3; 35 kDa lectin; IgE-binding protein; MAC-2 antigen; carbohydrate-binding protein 35; galactose-specific lectin 3; laminin-binding protein; lectin L-29
 UniProt Protein Name    Galectin-3
 UniProt Synonym Protein Names   
35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen
 Protein Family    Galectin
 UniProt Gene Name    LGALS3 [Similar Products]
 UniProt Synonym Gene Names    MAC2; Gal-3; CBP 35; GALBP [Similar Products]
 UniProt Entry Name    LEG3_HUMAN
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 NCBI Summary for LGALS3    This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
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 UniProt Comments for LGALS3    Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Cell surface; Motility/polarity/chemotaxis; Extracellular matrix

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: extracellular matrix; spliceosome; extracellular space; proteinaceous extracellular matrix; membrane; cytoplasm; mitochondrial inner membrane; plasma membrane; immunological synapse; nucleus; external side of plasma membrane

Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; positive chemotaxis; epithelial cell differentiation; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development
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Product References and Citations for LGALS3 recombinant protein

   Barondes SH., et al. (1994) J Biol Chem. 269(33):20807-10.
Kadrofske MM., et al. (1998) Arch Biochem Biophys. 349(1):7-20.
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 Research Articles on LGALS3    1. Galectin-3 plasma levels predicts the risk of ventricular tachycardia in heart failure patients with implantable defibrillators.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LGALS3 recombinant proteinPathways associated with LGALS3 recombinant protein
 Reference Product  PubMed Publications
 LGALS3BP recombinant protein  >39 publications with LGALS3 and LGALS3BP
 EGFR recombinant protein  >35 publications with LGALS3 and EGFR
 MUC1 recombinant protein  >26 publications with LGALS3 and MUC1
 KRAS recombinant protein  >9 publications with LGALS3 and KRAS
 GEMIN4 recombinant protein  >5 publications with LGALS3 and GEMIN4
 PRKCSH recombinant protein  >2 publications with LGALS3 and PRKCSH
 NRAS recombinant protein  >2 publications with LGALS3 and NRAS
 HRAS recombinant protein  >2 publications with LGALS3 and HRAS
 DDOST recombinant protein  >2 publications with LGALS3 and DDOST
 SUFU recombinant protein  >1 publications with LGALS3 and SUFU
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Diseases associated with LGALS3 recombinant proteinOrgans/Tissues associated with LGALS3 recombinant protein
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >544 publications with LGALS3 and Carcinoma
 Inflammation Antibodies  >274 publications with LGALS3 and Inflammation
 Adenocarcinoma Antibodies  >227 publications with LGALS3 and Adenocarcinoma
 Fibrosis Antibodies  >161 publications with LGALS3 and Fibrosis
 Disease Models, Animal Antibodies  >153 publications with LGALS3 and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >150 publications with LGALS3 and Neoplasm Metastasis
 Adenoma Antibodies  >137 publications with LGALS3 and Adenoma
 Heart Diseases Antibodies  >135 publications with LGALS3 and Heart Diseases
 Brain Diseases Antibodies  >114 publications with LGALS3 and Brain Diseases
 Breast Neoplasms Antibodies  >112 publications with LGALS3 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >591 publications with LGALS3 and Blood
 Thyroid Antibodies  >297 publications with LGALS3 and Thyroid
 Heart Antibodies  >207 publications with LGALS3 and Heart
 Vascular Antibodies  >179 publications with LGALS3 and Vascular
 Brain Antibodies  >152 publications with LGALS3 and Brain
 Kidney Antibodies  >127 publications with LGALS3 and Kidney
 Lung Antibodies  >126 publications with LGALS3 and Lung
 Liver Antibodies  >125 publications with LGALS3 and Liver
 Bone Antibodies  >113 publications with LGALS3 and Bone
 Muscle Antibodies  >93 publications with LGALS3 and Muscle
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