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VP1 recombinant protein :: Genome polyprotein VP1 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1220686
SDS-Page
Unit / Price
0.01 mg (Yeast)  /  $110 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $190 +1 FREE 8GB USB
0.1 mg (Yeast)  /  $285 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $460 +1 FREE 8GB USB
0.05 mg (E-Coli)  /  $690 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $750 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $930 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $970 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $1,020 +1 FREE 8GB USB
1 mg (Yeast)  /  $1,180 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,205 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,220 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,445 +1 FREE 8GB USB
0.5 mg (Baculovirus)  /  $1,755 +2 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $1,830 +2 FREE 8GB USB
1 mg (Baculovirus)  /  $2,340 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Genome polyprotein VP1, Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human rhinovirus 1A Genome polyprotein VP1

 Product Gene Name   

VP1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    571-857aa
 Sequence    NPVENYIDEV LNEVLVVPNI KESHHTTSNS APLLDAAETG HTSNVQPEDA IETRYVITSQ TRDEMSIESF LGRSGCVHIS RIKVDYTDYN GQDINFTKWK ITLQEMAQIR RKFELFTYVR FDSEITLVPC IAGRGDDIGH IVMQYMYVPP GAPIPSKRND FSWQSGTNMS IFWQHGQPFP RFSLPFLSIA SAYYMFYDGY DGDNTSSKYG SVVTNDMGTI CSRIVTEKQK HSVVITTHIY HKAKHTKAWC PRPPRAVPYT HSHVTNYMPE TGDVTTAIVR RNTITTA
 3D Structure    ModBase 3D Structure for P23008
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of VP1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for VP1 recombinant protein

   Capsid protein VP1: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor to provide virion attachment to target host cells. This attachment induces virion internalization. Tyrosine kinases are probably involved in the entry process. After binding to its receptor, the capsid undergoes conformational changes. Capsid protein VP1 N-terminus (that contains an amphipathic alpha-helix) and capsid protein VP4 are externalized. Together, they shape a pore in the host membrane through which viral genome is translocated to host cell cytoplasm. After genome has been released, the channel shrinks. Capsid protein VP2: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP3: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP4: Lies on the inner surface of the capsid shell. After binding to the host receptor, the capsid undergoes conformational changes. Capsid protein VP4 is released, Capsid protein VP1 N-terminus is externalized, and together, they shape a pore in the host membrane through which the viral genome is translocated into the host cell cytoplasm. After genome has been released, the channel shrinks. Capsid protein VP0: Component of immature procapsids, which is cleaved into capsid proteins VP4 and VP2 after maturation. Allows the capsid to rain inactive before the maturation step. Protein 2A: Cysteine protease that cleaves viral polyprotein and specific host proteins. It is responsible for the cleavage between the P1 and P2 regions, first cleavage occurring in the polyprotein. Cleaves also the host translation initiation factor EIF4G1, in order to shut down the capped cellular mRNA translation. Inhibits the host nucleus-cytoplasm protein and RNA trafficking by cleaving host mbers of the nuclear pores. Protein 2B: Plays an essential role in the virus replication cycle by acting as a viroporin. Creates a pore in the host reticulum endoplasmic and as a consequence releases Ca2+ in the cytoplasm of infected cell. In turn, high levels of cyctoplasmic calcium may trigger membrane trafficking and transport of viral ER-associated proteins to viroplasms, sites of viral genome replication. Protein 2C: Induces and associates with structural rearrangents of intracellular membranes. Displays RNA-binding, nucleotide binding and NTPase activities. May play a role in virion morphogenesis and viral RNA encapsidation by interacting with the capsid protein VP3. Protein 3AB: Localizes the viral replication complex to the surface of mbranous vesicles. Together with protein 3CD binds the Cis-Active RNA element (CRE) which is involved in RNA synthesis initiation. Acts as a cofactor to stimulate the activity of 3D polymerase, maybe through a nucleid acid chaperone activity. Protein 3A: Localizes the viral replication complex to the surface of mbranous vesicles. It inhibits host cell endoplasmic reticulum-to-Golgi apparatus transport and causes the dissassembly of the Golgi complex, possibly through GBF1 interaction. This would result in depletion of MHC, trail receptors and IFN receptors at the host cell surface. Viral protein genome-linked: acts as a primer for viral RNA replication and rains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU. The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome. VPg may be roved in the cytoplasm by an unknown enzyme termed "unlinkase". VPg is not cleaved off virion genomes because replicated genomic RNA are encapsidated at the site of replication. Protein 3CD: Is involved in the viral replication complex and viral polypeptide maturation. It exhibits protease activity with a specificity and catalytic efficiency that is different from protease 3C. Protein 3CD lacks polymerase activity. The 3C domain in the context of protein 3CD may have an RNA binding activity. Protease 3C: cleaves host DDX58/RIG-I and thus contributes to the inhibition of type I interferon production. Cleaves also host PABPC1. RNA-directed RNA polymerase: Replicates the viral genomic RNA on the surface of intracellular membranes. May form linear arrays of subunits that propagate along a strong head-to-tail interaction called interface-I. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced mbranous vesicles, creating a dsRNA genomic replication form. This dsRNA is then used as template to synthesize positive stranded RNA genomes. ss+RNA genomes are either translated, replicated or encapsidated.
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 SDS-Page of VP1 recombinant protein    VP1 recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for VP1. It may not necessarily be applicable to this product.
 NCBI GI #    527504080
 NCBI Accession #    P23008.3 [Other Products]
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 UniProt Primary Accession #    P23008 [Other Products]
 UniProt Secondary Accession #    B9V432 [Other Products]
 Molecular Weight    34.6kD
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 NCBI Official Full Name    Genome polyprotein
 UniProt Protein Name    Genome polyprotein
 UniProt Synonym Protein Names   
P3Protein 3ABP2P1Capsid protein VP0Alternative name(s):VP4-VP2
 Protein Family    Capsid protein
 UniProt Gene Name    P2A [Similar Products]
 UniProt Synonym Gene Names    P2B; P2C; P3A; VPg; P3B; P3C; RdRp; 3Dpol; 3D [Similar Products]
 UniProt Entry Name    POLG_HRV1A
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 UniProt Comments for VP1    Capsid protein VP1: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor to provide virion attachment to target host cells. This attachment induces virion internalization. Tyrosine kinases are probably involved in the entry process. After binding to its receptor, the capsid undergoes conformational changes. Capsid protein VP1 N-terminus (that contains an amphipathic alpha-helix) and capsid protein VP4 are externalized. Together, they shape a pore in the host membrane through which viral genome is translocated to host cell cytoplasm. After genome has been released, the channel shrinks ().
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Product References and Citations for VP1 recombinant protein

   Sequencing and analyses of all known human rhinovirus genomes reveal structure and evolution.Palmenberg A.C., Spiro D., Kuzmickas R., Wang S., Djikeng A., Rathe J.A., Fraser-Liggett C.M., Liggett S.B.Science 324:55-59(2009) Crystal structure of human rhinovirus serotype 1A (HRV1A) .Kim S., Smith T.J., Chapman M.S., Rossmann M.G., Pevear D., Dutko F.J., Felock P.J., Diana G.D., McKinlay M.A.J. Mol. Biol. 210:91-111(1989) Productive entry pathways of human rhinoviruses.Fuchs R., Blaas D.Adv. Virol. 2012:826301-826301(2012)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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