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GAPDH recombinant protein :: Glyceraldehyde-3-phosphate dehydrogenase Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS717049
SDS-PAGE
Unit / Price
0.01 mg  /  $110 +1 FREE 8GB USB
0.05 mg  /  $190 +1 FREE 8GB USB
0.1 mg  /  $285 +1 FREE 8GB USB
0.2 mg  /  $460 +1 FREE 8GB USB
0.5 mg  /  $750 +1 FREE 8GB USB
1 mg  /  $1,180 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Recombinant Protein

 Also Known As   

Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase

 Product Synonym Names    Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)
 Product Gene Name   

GAPDH recombinant protein

[Similar Products]
 Product Synonym Gene Name    GAPD; CDABP0047; OK; SW-cl.12 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    3-335aa; Partial
 Sequence    KVKVGVNGFG RIGRLVTRAA FNSGKVDIVA INDPFIDLNY MVYMFQYDST HGKFHGTVKA ENGKLVINGN PITIFQERDP SKIKWGDAGA EYVVESTGVF TTMEKAGAHL QGGAKRVIIS APSADAPMFV MGVNHEKYDN SLKIISNASC TTNCLAPLAK VIHDNFGIVE GLMTTVHAIT ATQKTVDGPS GKLWRDGRGA LQNIIPASTG AAKAVGKVIP ELNGKLTGMA FRVPTANVSV VDLTCRLEKP AKYDDIKKVV KQASEGPLKG ILGYTEHQVV SSDFNSDTHS STFDAGAGIA LNDHFVKLIS WYDNEFGYSN RVVDLMAHMA SKE
 OMIM    138400
 3D Structure    ModBase 3D Structure for P04406
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 Host    E Coli
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of GAPDH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for GAPDH recombinant protein

   Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assbles into the GAIT complex which binds to st loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. 3 Publications
 Product Categories/Family for GAPDH recombinant protein    Apoptosis
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 SDS-PAGE of GAPDH recombinant protein    GAPDH recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.
 NCBI GI #    378404908
 NCBI GeneID    2597
 NCBI Accession #    NP_001243728.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001256799.2 [Other Products]
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 UniProt Primary Accession #    P04406 [Other Products]
 UniProt Secondary Accession #    P00354; Q53X65; E7EUT4 [Other Products]
 UniProt Related Accession #    P04406 [Other Products]
 Molecular Weight    63.2kD
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 NCBI Official Full Name    glyceraldehyde-3-phosphate dehydrogenase isoform 2
 NCBI Official Synonym Full Names    glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Symbol    GAPDH [Similar Products]
 NCBI Official Synonym Symbols   
G3PD; GAPD; HEL-S-162eP
[Similar Products]
 NCBI Protein Information    glyceraldehyde-3-phosphate dehydrogenase
 UniProt Protein Name    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Synonym Protein Names   
Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)
 Protein Family    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Gene Name    GAPDH [Similar Products]
 UniProt Synonym Gene Names    GAPD; GAPDH [Similar Products]
 UniProt Entry Name    G3P_HUMAN
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 NCBI Summary for GAPDH    This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
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 UniProt Comments for GAPDH    GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma

Protein type: EC 1.2.1.12; Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; lipid particle; membrane; microtubule cytoskeleton; nuclear membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; ribonucleoprotein complex; vesicle

Molecular Function: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; identical protein binding; microtubule binding; NAD binding; NADP binding; protein binding

Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process; microtubule cytoskeleton organization and biogenesis; negative regulation of translation; neuron apoptosis; protein stabilization
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Product References and Citations for GAPDH recombinant protein

   The glyceraldehyde 3 phosphate dehydrogenase gene family structure of a human cDNA and of an X chromosome linked pseudogene; amazing complexity of the gene family in mouse.Hanauer A., Mandel J.-L.EMBO J. 3:2627-2633(1984)
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 Research Articles on GAPDH    1. The level of GAPDH-AP DNA adduct formation depends on oxidation of the protein SH-groups; disulfide bond reduction in GAPDH leads to the loss of its ability to form the adducts with AP DNA
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GAPDH recombinant proteinPathways associated with GAPDH recombinant protein
 Reference Product  PubMed Publications
 RPL13A recombinant protein  >66 publications with GAPDH and RPL13A
 UBC recombinant protein  >64 publications with GAPDH and UBC
 PGK1 recombinant protein  >53 publications with GAPDH and PGK1
 ENO1 recombinant protein  >12 publications with GAPDH and ENO1
 ALDOA recombinant protein  >10 publications with GAPDH and ALDOA
 HTT recombinant protein  >7 publications with GAPDH and HTT
 BPGM recombinant protein  >1 publications with GAPDH and BPGM
 PGK2 recombinant protein  >1 publications with GAPDH and PGK2
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 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Diseases associated with GAPDH recombinant proteinOrgans/Tissues associated with GAPDH recombinant protein
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >295 publications with GAPDH and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >264 publications with GAPDH and Cardiovascular Diseases
 Necrosis Antibodies  >217 publications with GAPDH and Necrosis
 Brain Diseases Antibodies  >216 publications with GAPDH and Brain Diseases
 Inflammation Antibodies  >201 publications with GAPDH and Inflammation
 Heart Diseases Antibodies  >155 publications with GAPDH and Heart Diseases
 Liver Diseases Antibodies  >148 publications with GAPDH and Liver Diseases
 Kidney Diseases Antibodies  >101 publications with GAPDH and Kidney Diseases
 Liver Neoplasms Antibodies  >83 publications with GAPDH and Liver Neoplasms
 Lung Neoplasms Antibodies  >78 publications with GAPDH and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >836 publications with GAPDH and Blood
 Muscle Antibodies  >557 publications with GAPDH and Muscle
 Brain Antibodies  >507 publications with GAPDH and Brain
 Liver Antibodies  >415 publications with GAPDH and Liver
 Heart Antibodies  >304 publications with GAPDH and Heart
 Vascular Antibodies  >291 publications with GAPDH and Vascular
 Kidney Antibodies  >262 publications with GAPDH and Kidney
 Lung Antibodies  >236 publications with GAPDH and Lung
 Bone Antibodies  >196 publications with GAPDH and Bone
 Nerve Antibodies  >145 publications with GAPDH and Nerve
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