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PYGM recombinant protein :: Glycogen Phosphorylase, Muscle (PYGM) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2011599 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $150 +1 FREE 8GB USB
0.05 mg  /  $295 +1 FREE 8GB USB
0.1 mg  /  $465 +1 FREE 8GB USB
0.2 mg  /  $580 +1 FREE 8GB USB
0.5 mg  /  $1,120 +1 FREE 8GB USB
1 mg  /  $1,665 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glycogen Phosphorylase, Muscle (PYGM), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Glycogen Phosphorylase, Muscle (PYGM)

 Product Gene Name   

PYGM recombinant protein

[Similar Products]
 Matching Pairs    Antibody: Glycogen Phosphorylase, Muscle (PYGM) (MBS2002939)
Antigen: Glycogen Phosphorylase, Muscle (PYGM) (MBS2011599)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-RKQISV RGLA GVENVSELKK NFNRHLHFTL VKDRNVATPR DYYFALAHTV RDHLVGRWIR TQQHYYEKDP KRIYYLSLEF YMGRTLQNTM VNLALENACD EATYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT LGLAAYGYGI RYEFGIFNQK ICGGWQMEEA DDWLRYG
 Chromosome Location    Chromosome: 19; NC_000085.6 (6384429..6398459). Location: 19 A; 19 4.53 cM
 3D Structure    ModBase 3D Structure for Q9WUB3
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 Host    E Coli
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 Purity/Purification    >98%
 Form/Format    20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    Arg11~Gly187 with N-terminal His-Tag
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Predicted isoelectric point    6.0
  Predicted Molecular Mass    21.7kDa
 Accurate Molecular Mass    26kDa as determined by SDS-PAGE reducing conditions.
 Usage    Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
 Tissue Specificity    Skeletal Muscle, Brain, Heart, Kidney.
 Traits    Freeze-dried powder
 Note    The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affect the charge of the protein.
3. Post-translational modification:Phosphorylation, glycosylation, methylation etc.
4. Post-translation clevage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of PYGM recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for PYGM recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

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 Test Data of PYGM recombinant protein    PYGM recombinant protein Test Data image
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 SDS-PAGE of PYGM recombinant protein    PYGM recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for PYGM. It may not necessarily be applicable to this product.
 NCBI GI #    6755256
 NCBI GeneID    19309
 NCBI Accession #    NP_035354.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_011224.1 [Other Products]
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 UniProt Primary Accession #    Q9WUB3 [Other Products]
 UniProt Related Accession #    Q9WUB3 [Other Products]
 Molecular Weight    Predicted Molecular Weight: 22.0kDa
Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.
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 NCBI Official Full Name    glycogen phosphorylase, muscle form
 NCBI Official Synonym Full Names    muscle glycogen phosphorylase
 NCBI Official Symbol    Pygm [Similar Products]
 NCBI Official Synonym Symbols   
PG; AI115133
[Similar Products]
 NCBI Protein Information    glycogen phosphorylase, muscle form; myophosphorylase
 UniProt Protein Name    Glycogen phosphorylase, muscle form
 UniProt Synonym Protein Names   
Myophosphorylase
 Protein Family    Glycogen phosphorylase
 UniProt Gene Name    Pygm [Similar Products]
 UniProt Entry Name    PYGM_MOUSE
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 UniProt Comments for PYGM    PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Protein type: Phosphorylase; Transferase; EC 2.4.1.1; Carbohydrate Metabolism - starch and sucrose; Endoplasmic reticulum

Cellular Component: sarcoplasmic reticulum; cytoplasm; Z disc

Molecular Function: transferase activity; glycogen phosphorylase activity; transferase activity, transferring glycosyl groups; phosphorylase activity; nucleotide binding; drug binding; carbohydrate binding; catalytic activity; pyridoxal phosphate binding; AMP binding

Biological Process: cellular calcium ion homeostasis; glycogen metabolic process; response to organic substance; glycogen catabolic process; response to cAMP; metabolic process; carbohydrate metabolic process; response to hypoxia
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 Research Articles on PYGM    1. this is the first successful attempt to develop a genetically modified animal model of McArdle disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PYGM recombinant proteinDiseases associated with PYGM recombinant protein
 Products by Pathway  Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycogen Metabolism Pathway antibodies  Glycogen Metabolism Pathway Diagram
 Glycogen Breakdown (glycogenolysis) Pathway antibodies  Glycogen Breakdown (glycogenolysis) Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram
 Starch And Sucrose Metabolism Pathway antibodies  Starch And Sucrose Metabolism Pathway Diagram
 Starch And Sucrose Metabolism Pathway antibodies  Starch And Sucrose Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >64 publications with PYGM and Neoplasms
 Glycogen Storage Disease Type V Antibodies  >57 publications with PYGM and Glycogen Storage Disease Type V
 Nervous System Diseases Antibodies  >22 publications with PYGM and Nervous System Diseases
 Adenocarcinoma Antibodies  >15 publications with PYGM and Adenocarcinoma
 Hyperplasia Antibodies  >10 publications with PYGM and Hyperplasia
 Muscular Diseases Antibodies  >8 publications with PYGM and Muscular Diseases
 Breast Neoplasms Antibodies  >6 publications with PYGM and Breast Neoplasms
 Lung Neoplasms Antibodies  >6 publications with PYGM and Lung Neoplasms
 Carcinoma, Squamous Cell Antibodies  >3 publications with PYGM and Carcinoma, Squamous Cell
 Skin Neoplasms Antibodies  >2 publications with PYGM and Skin Neoplasms
Organs/Tissues associated with PYGM recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >83 publications with PYGM and Muscle
 Lung Antibodies  >8 publications with PYGM and Lung
 Liver Antibodies  >8 publications with PYGM and Liver
 Skin Antibodies  >4 publications with PYGM and Skin
 Brain Antibodies  >4 publications with PYGM and Brain
 Eye Antibodies  >4 publications with PYGM and Eye
 Heart Antibodies  >4 publications with PYGM and Heart
 Stomach Antibodies  >2 publications with PYGM and Stomach
 Bone Antibodies  >1 publications with PYGM and Bone
 Spinal Cord Antibodies  >1 publications with PYGM and Spinal Cord
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