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H5 recombinant protein :: H5 (H5N1) (A/Vietnam/1203/2004) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS434010
Testing Data
Unit / Price
0.05 mg  /  $350 +1 FREE 8GB USB
 
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 Product Name   

H5 (H5N1) (A/Vietnam/1203/2004), Recombinant Protein

★Popular Item★
 Also Known As   

H5 (H5N1) (aa 18-530)(A/Vietnam/1203/2004)

 Product Synonym Names    H5 (H5N1) (A/Vietnam/1203/2004); glycosylated recombinant protein; HA (H5N1)(aa 18-530) (A/Vietnam/1203/2004); H5 (H5N1), 6xHis tagged Hemagglutinin (A/Vietnam/1203/2004) (aa 18-530), glycosylated recombinant protein
 Product Gene Name   

H5 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Host    293 cell culture
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 Purity/Purification    > = 95% (SDS-PAGE)
 Concentration    1 ug/ul in PBS. (lot specific)
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 Viral Protein    C-terminal 6xHis tagged HA (H5N1) (A/Vietnam/1203/2004) protein (amino acid 18-530) with a deletion at its cleaveage site (deltaRRRKKR) (Genebank accession# AAW80717). Majority of the protein exists as trimer/oligomer.
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 Preparation and Storage    Store at -20 degree C. Stable for 3-months from the date of shipment when kept at 4 degree C. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of H5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for H5 purchase    Hemagglutinin, abbreviated HA for influenza virus proteins, is a useful tool in a genome workbench setting for development of vaccines to conserved regions. H5 HA is a recombinant protein purified by 6x his tags from mammalian expression vectormune‚ and developed to the Genbank C-term sequence with a cleavage site deletion. The H5N1 virus is an emerging biological threat and the FDA is looking for any resources leading to a potential vaccine to test in mice. Using BLAST database, Pubmed searches and basic local alignment to related HA products reveals conserved domain gene homology, increasing the possibility of success. Using the H5 product in cell based screening assays or development of a robust neutralizing antibody combined with a genetics amp medicine approach may identify lead compounds, which may further be optimized in chemicals amp bioassays - see Pubchem for text protocols describing this methodology.
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Product Description specifically for H5 recombinant protein

   Description: 6xHis tagged H5 (H5N1) (A/Vietnam/1203/2004) protein (aa 18-500) with a deletion at its cleaveage site (ąÜRRRKKR) (Genebank accession# AAW80717)
Viral protein purified from 293 cell culture.
Introduction: Influenza hemagglutinin (HA) is a type of hemagglutinin found on the surface of the influenza viruses. HA is an antigenic glycoprotein, like all other hemagglutinins, it causes red blood cells to agglutinate. HA is responsible for binding the virus to the cell that is being infected. HA proteins bind to cells with sialic acid on the membranes, such as cells in the upper respiratory tract or erythrocytes.
HA is a homotrimeric integral membrane glycoprotein. HA monomer is synthesized as a single polypeptide that is subsequently cleaved into two smaller polypeptides, the HA1 and HA2 subunits. Each HA monomer consists of a long, helical chain anchored in the membrane by HA2 and topped by a large HA1 globule.
 Product Categories/Family for H5 recombinant protein    Viral Recombinant Proteins; Influenza
 Applications Tested/Suitable for H5 recombinant protein   

Western blot standard, antibody ELISA, antigen, etc.

 Application Notes for H5 recombinant protein    SDS-PAGE Data: purified HA (H5N1)(A/Vietnam/1203/2004)(aa18-530) protein
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 Testing Data of H5 recombinant protein    SDS - PAGE : purified H A (H5N1) (A/Vietnam/1203/2004) (aa18 - 530)
H5 recombinant protein Testing Data image
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NCBI/Uniprot data below describe general gene information for H5. It may not necessarily be applicable to this product.
 NCBI GI #    58618438
 NCBI Accession #    AAW80717.1 [Other Products]
 Molecular Weight    64,461 Da
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 NCBI Official Full Name    hemagglutinin HA
 UniProt Protein Name    Hemagglutinin
 Protein Family    Probable tyrosine phosphatase protein
 UniProt Gene Name    HA [Similar Products]
 UniProt Entry Name    Q5EP31_9INFA
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 UniProt Comments for H5    Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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