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HMGB1 recombinant protein :: High-Mobility Group Box 1 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS143251 HMGB1 recombinant protein
Unit / Price
0.01 mg  /  $140 +1 FREE 8GB USB
0.05 mg  /  $205 +1 FREE 8GB USB
1 mg  /  $1,800 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

High-Mobility Group Box 1 (HMGB1), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human High-Mobility Group Box 1

 Product Synonym Names    HMGB1 Human; High-Mobility Group Box 1 Human Recombinant; HMG1; HMG3; SBP-1; Amphoterin; HMGB1; High-Mobility Group Box 1
 Product Gene Name   

HMGB1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKEEEEDE EDEEDEEEEE DEEDEDEEED DDDELEHHHH HH
 OMIM    163905
 3D Structure    ModBase 3D Structure for P09429
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 Host    E Coli
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 Purity/Purification    Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
 Form/Format    The HMG1 (1 mg/ml) was lyophilized after extensive dialyses against 1x PBS pH-7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
 Concentration    1mg/ml (lot specific)
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 Solubility    It is recommended to reconstitute the lyophilized HMGB1 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
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 Preparation and Storage    Lyophilized HMGB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution HMGB1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
 Other Notes    Small volumes of HMGB1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for HMGB1 recombinant protein

   Description: HMG1 Human Recombinant fused with 6X His tag produced in E Coli is a single, non-glycosylated, polypeptide chain containing 223 amino acids and having a molecular mass of 26 kDa.The HMGB-1 is purified by proprietary chromatographic techniques.

Introduction: High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.
 Product Categories/Family for HMGB1 recombinant protein    RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; High-Mobility Group
NCBI/Uniprot data below describe general gene information for HMGB1. It may not necessarily be applicable to this product.
 NCBI GI #    4504425
 NCBI GeneID    3146
 NCBI Accession #    NP_002119.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002128.4 [Other Products]
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 UniProt Primary Accession #    P09429 [Other Products]
 UniProt Secondary Accession #    Q14321; Q5T7C3; Q6IBE1; A5D8W9 [Other Products]
 UniProt Related Accession #    P09429 [Other Products]
 Molecular Weight    24,894 Da
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 NCBI Official Full Name    high mobility group protein B1
 NCBI Official Synonym Full Names    high mobility group box 1
 NCBI Official Symbol    HMGB1 [Similar Products]
 NCBI Official Synonym Symbols   
HMG1; HMG3; SBP-1
[Similar Products]
 NCBI Protein Information    high mobility group protein B1; Amphoterin; HMG-1; Sulfoglucuronyl carbohydrate binding protein; high mobility group protein 1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1
 UniProt Protein Name    High mobility group protein B1
 UniProt Synonym Protein Names   
High mobility group protein 1; HMG-1
 Protein Family    High mobility group protein
 UniProt Gene Name    HMGB1 [Similar Products]
 UniProt Synonym Gene Names    HMG1; HMG-1 [Similar Products]
 UniProt Entry Name    HMGB1_HUMAN
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 UniProt Comments for HMGB1    HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus

Molecular Function: protein binding; RAGE receptor binding; double-stranded DNA binding; damaged DNA binding; cytokine activity; chromatin binding; DNA bending activity; transcription factor activity; transcription factor binding; single-stranded DNA binding; chemoattractant activity

Biological Process: negative regulation of transcriptional preinitiation complex assembly; V(D)J recombination; DNA topological change; positive regulation of apoptosis; apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; innate immune response; DNA ligation during DNA repair; dendritic cell chemotaxis; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; positive regulation of DNA binding; cell structure disassembly during apoptosis
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 Research Articles on HMGB1    1. We propose that HMGB1 released from cancer cells induces the expression of pro-inflammatory cytokines in peritoneal fibroblasts through the HMGB1-TLR2/4 pathway.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HMGB1 recombinant proteinPathways associated with HMGB1 recombinant protein
 Reference Product  PubMed Publications
 TNF recombinant protein  >458 publications with HMGB1 and TNF
 TLR4 recombinant protein  >349 publications with HMGB1 and TLR4
 TP53 recombinant protein  >8 publications with HMGB1 and TP53
 AGER recombinant protein  >4 publications with HMGB1 and AGER
 DIF recombinant protein  >2 publications with HMGB1 and DIF
 PLAT recombinant protein  >1 publications with HMGB1 and PLAT
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies  Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Induced DNA Fragmentation Pathway antibodies  Apoptosis Induced DNA Fragmentation Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies  DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
Diseases associated with HMGB1 recombinant proteinOrgans/Tissues associated with HMGB1 recombinant protein
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1337 publications with HMGB1 and Inflammation
 Necrosis Antibodies  >747 publications with HMGB1 and Necrosis
 Sepsis Antibodies  >492 publications with HMGB1 and Sepsis
 Disease Models, Animal Antibodies  >383 publications with HMGB1 and Disease Models, Animal
 Lung Diseases Antibodies  >224 publications with HMGB1 and Lung Diseases
 Lung Injury Antibodies  >199 publications with HMGB1 and Lung Injury
 Liver Diseases Antibodies  >175 publications with HMGB1 and Liver Diseases
 Heart Diseases Antibodies  >128 publications with HMGB1 and Heart Diseases
 Myocardial Ischemia Antibodies  >105 publications with HMGB1 and Myocardial Ischemia
 Kidney Diseases Antibodies  >85 publications with HMGB1 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1113 publications with HMGB1 and Blood
 Liver Antibodies  >414 publications with HMGB1 and Liver
 Lung Antibodies  >379 publications with HMGB1 and Lung
 Brain Antibodies  >307 publications with HMGB1 and Brain
 Vascular Antibodies  >291 publications with HMGB1 and Vascular
 Muscle Antibodies  >179 publications with HMGB1 and Muscle
 Heart Antibodies  >164 publications with HMGB1 and Heart
 Kidney Antibodies  >157 publications with HMGB1 and Kidney
 Bone Antibodies  >128 publications with HMGB1 and Bone
 Thymus Antibodies  >104 publications with HMGB1 and Thymus
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