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HIF1A recombinant protein :: Hypoxia-Inducible Factor-1 Alpha Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS144261 HIF1A recombinant protein
Unit / Price
0.001 mg  /  $140 +1 FREE 8GB USB
0.005 mg  /  $205 +1 FREE 8GB USB
0.05 mg  /  $1,220 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hypoxia-Inducible Factor-1 Alpha (HIF1A), Recombinant Protein

 Also Known As   

Recombinant Human Hypoxia-Inducible Factor-1 Alpha (85 a.a.)

 Product Synonym Names    HIF1A Human (85 a.a.); Hypoxia-Inducible Factor-1 Alpha (85 a.a.) Human Recombinant; Hypoxia-inducible factor 1 alpha; HIF-1 alpha; HIF1 alpha; ARNT-interacting protein; Member of PAS protein 1; Basic-helix-loop-helix-PAS protein MOP1; HIF1A; MOP1; HIF1; PASD8; HIF-1A; HIF1A (85 a.a.)
 Product Gene Name   

HIF1A recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHH HHH SSGLVPRGSH MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMK
 OMIM    603348
 3D Structure    ModBase 3D Structure for Q16665
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 Host    E Coli
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 Purity/Purification    Greater than 80.0% as determined by SDS-PAGE.
 Form/Format    HIF1A Human (0.25mg/ml) solution containing 20mM Tris buffer(pH 8.0), 20% glycerol, 1mM DTT, 0.2M NaCl and 1mM EDTA.
Sterile filtered colorless solution.
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 Preparation and Storage    HIF1A Human although stable at 4 degree C for 1 week, should be stored desiccated below -18 degree C. Please prevent freeze thaw cycles.
 Other Notes    Small volumes of HIF1A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for HIF1A recombinant protein

   Description: HIF1A Recombinant Human produced in E Coli is a single, non-glycosylated polypeptide chain containing 105 amino acids (1-85 a.a.) and having a molecular mass of 11.8 kDa. The HIF1A is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Introduction: HIF1A has a role as a master transcriptional monitor of the adaptive response to hypoxia. Under hypoxic conditions HIF1A activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and genes whose protein products increase oxygen release or facilitate metabolic adaptation to hypoxia. HIF1A functions as an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.
 Product Categories/Family for HIF1A recombinant protein    RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Hypoxia-Inducible Factor
NCBI/Uniprot data below describe general gene information for HIF1A. It may not necessarily be applicable to this product.
 NCBI GI #    340545531
 NCBI GeneID    3091
 NCBI Accession #    NP_001230013.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001243084.1 [Other Products]
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 UniProt Primary Accession #    Q16665 [Other Products]
 UniProt Secondary Accession #    Q53XP6; Q96PT9; Q9UPB1; C0LZJ3 [Other Products]
 UniProt Related Accession #    Q16665 [Other Products]
 Molecular Weight    95,634 Da
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 NCBI Official Full Name    hypoxia-inducible factor 1-alpha isoform 3
 NCBI Official Synonym Full Names    hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 NCBI Official Symbol    HIF1A [Similar Products]
 NCBI Official Synonym Symbols   
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
[Similar Products]
 NCBI Protein Information    hypoxia-inducible factor 1-alpha; ARNT interacting protein; ARNT-interacting protein; HIF-1-alpha; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1
 UniProt Protein Name    Hypoxia-inducible factor 1-alpha
 UniProt Synonym Protein Names   
ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8
 Protein Family    Hypoxia-inducible factor
 UniProt Gene Name    HIF1A [Similar Products]
 UniProt Synonym Gene Names    BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78 [Similar Products]
 UniProt Entry Name    HIF1A_HUMAN
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 NCBI Summary for HIF1A    This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
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 UniProt Comments for HIF1A    HIF1A: a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability. Interacts with EP300 (via TAZ-type 1 domains); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domains). Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with TSGA10. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia- mimicking conditions. Interacts with USP20. Interacts with RACK1; promotes HIF1A ubiquitination and proteasome- mediated degradation. Interacts (via N-terminus) with USP19. Under reduced oxygen tension. Induced also by various receptor-mediated factors such as growth factors, cytokines, and circulatory factors such as PDGF, EGF, FGF2, IGF2, TGFB1, HGF, TNF, IL1B, angiotensin-2 and thrombin. However, this induction is less intense than that stimulated by hypoxia. Repressed by HIPK2 and LIMD1. Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; Autophagy

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nuclear speck; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone deacetylase binding; Hsp90 protein binding; protein kinase binding; transcription factor binding; histone acetyltransferase binding; protein binding; signal transducer activity; enzyme binding; sequence-specific DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription factor activity; nuclear hormone receptor binding

Biological Process: lactation; oxygen homeostasis; embryonic placenta development; response to muscle activity; cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; glucose homeostasis; signal transduction; positive regulation of vascular endothelial growth factor receptor signaling pathway; muscle maintenance; negative regulation of bone mineralization; elastin metabolic process; connective tissue replacement during inflammatory response; axon transport of mitochondrion; regulation of transcription, DNA-dependent; visual learning; angiogenesis; heart looping; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; neural crest cell migration; negative regulation of growth; hemoglobin biosynthetic process; positive regulation of neuroblast proliferation; Notch signaling pathway; negative regulation of TOR signaling pathway; regulation of transforming growth factor-beta2 production; collagen metabolic process; embryonic hemopoiesis; positive regulation of nitric-oxide synthase activity; positive regulation of erythrocyte differentiation; B-1 B cell homeostasis; digestive tract morphogenesis; mRNA transcription from RNA polymerase II promoter; positive regulation of chemokine production; positive regulation of angiogenesis; neural fold elevation formation; regulation of gene expression; cartilage development; positive regulation of hormone biosynthetic process; positive regulation of glycolysis; lactate metabolic process; response to hypoxia; epithelial to mesenchymal transition; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development
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 Research Articles on HIF1A    1. High expression of HIF-1alpha is associated with sinonasal and laryngeal small-cell neuroendocrine carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HIF1A recombinant proteinPathways associated with HIF1A recombinant protein
 Reference Product  PubMed Publications
 VHL recombinant protein  >529 publications with HIF1A and VHL
 ARNT recombinant protein  >292 publications with HIF1A and ARNT
 EGLN1 recombinant protein  >144 publications with HIF1A and EGLN1
 EGLN3 recombinant protein  >68 publications with HIF1A and EGLN3
 HIF1AN recombinant protein  >59 publications with HIF1A and HIF1AN
 TP53 recombinant protein  >47 publications with HIF1A and TP53
 EP300 recombinant protein  >29 publications with HIF1A and EP300
 CREBBP recombinant protein  >14 publications with HIF1A and CREBBP
 UBC recombinant protein  >4 publications with HIF1A and UBC
 HSP90AA1 recombinant protein  >3 publications with HIF1A and HSP90AA1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
Diseases associated with HIF1A recombinant proteinOrgans/Tissues associated with HIF1A recombinant protein
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >1465 publications with HIF1A and Carcinoma
 Nervous System Diseases Antibodies  >702 publications with HIF1A and Nervous System Diseases
 Inflammation Antibodies  >562 publications with HIF1A and Inflammation
 Brain Diseases Antibodies  >516 publications with HIF1A and Brain Diseases
 Lung Diseases Antibodies  >459 publications with HIF1A and Lung Diseases
 Kidney Diseases Antibodies  >449 publications with HIF1A and Kidney Diseases
 Breast Neoplasms Antibodies  >447 publications with HIF1A and Breast Neoplasms
 Liver Diseases Antibodies  >392 publications with HIF1A and Liver Diseases
 Necrosis Antibodies  >392 publications with HIF1A and Necrosis
 Neoplasms, Experimental Antibodies  >357 publications with HIF1A and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >3422 publications with HIF1A and Vascular
 Blood Antibodies  >2010 publications with HIF1A and Blood
 Brain Antibodies  >774 publications with HIF1A and Brain
 Muscle Antibodies  >769 publications with HIF1A and Muscle
 Kidney Antibodies  >720 publications with HIF1A and Kidney
 Lung Antibodies  >660 publications with HIF1A and Lung
 Liver Antibodies  >622 publications with HIF1A and Liver
 Heart Antibodies  >577 publications with HIF1A and Heart
 Bone Antibodies  >458 publications with HIF1A and Bone
 Prostate Antibodies  >293 publications with HIF1A and Prostate
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