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HIF2a recombinant protein :: Hypoxia Inducible Factor 2 Alpha (HIF2a) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2009225 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment) HIF2a recombinant protein
Unit / Price
0.01 mg  /  $140 +1 FREE 8GB USB
0.05 mg  /  $265 +1 FREE 8GB USB
0.1 mg  /  $415 +1 FREE 8GB USB
0.2 mg  /  $510 +1 FREE 8GB USB
0.5 mg  /  $990 +1 FREE 8GB USB
1 mg  /  $1,465 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hypoxia Inducible Factor 2 Alpha (HIF2a), Recombinant Protein

 Also Known As   

Recombinant Hypoxia Inducible Factor 2 Alpha (HIF2a)

 Product Gene Name   

HIF2a recombinant protein

[Similar Products]
 Matching Pairs    Antibody: Hypoxia Inducible Factor 2 Alpha (HIF2a) (MBS2003798)
Antigen: Hypoxia Inducible Factor 2 Alpha (HIF2a) (MBS2009225)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-DAARCR RSKE TEVFYELAHE LPLPHSVSSH LDKASIMRLA ISFLRTHKLL SSVCSENESE AEADQQMDNL YLKALEGFIA VVTQDGDMIF LSENISKFMG LTQVELTGHS IFDFTHPCDH EEIRENLTLK NGSGFGKKSK DVSTERDFFM RMKCTVTNRG RTVNLKSATW KVLHCTGQVR VYNNCPPHSS LCGSKEPLLS CLIIMCEPIQ HPSHMDIPLD SKTFLSRHSM DMKFTYCDDR ILELIGYHPE ELLGRSAYEF YHALDSENMT KSHQNLCTKG QVVSGQYRML AKHGGYVWLE TQGTVIYNPR NLQPQC
 Chromosome Location    Chromosome: 17; NC_000083.6 (86753864..86833410). Location: 17 E4; 17
 3D Structure    ModBase 3D Structure for P97481
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    Asp21~Cys336 (Accession # P97481) with N-terminal His-Tag
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of HIF2a recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for HIF2a recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for HIF2a recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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NCBI/Uniprot data below describe general gene information for HIF2a. It may not necessarily be applicable to this product.
 NCBI GI #    178057356
 NCBI GeneID    13819
 NCBI Accession #    NP_034267.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_010137.3 [Other Products]
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 UniProt Primary Accession #    P97481 [Other Products]
 UniProt Secondary Accession #    O08787; O55046 [Other Products]
 UniProt Related Accession #    P97481 [Other Products]
 Molecular Weight    37.5kDa
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 NCBI Official Full Name    endothelial PAS domain-containing protein 1
 NCBI Official Synonym Full Names    endothelial PAS domain protein 1
 NCBI Official Symbol    Epas1 [Similar Products]
 NCBI Official Synonym Symbols   
HLF; HRF; MOP2; HIF2A; bHLHe73; HIF-2alpha
[Similar Products]
 NCBI Protein Information    endothelial PAS domain-containing protein 1; mHLF; EPAS-1; HIF2-alpha; HIF-2-alpha; Hif like protein; HIF-related factor; HIF1alpha-like factor; HIF1 alpha-like factor; HIF-1-alpha-like factor; HLF (HIF1alpha-like factor); hypoxia-inducible factor 2-alpha; hypoxia inducible transcription factor 2alpha
 UniProt Protein Name    Endothelial PAS domain-containing protein 1
 UniProt Synonym Protein Names   
HIF-1-alpha-like factor; HLF; mHLF; HIF-related factor; HRF; Hypoxia-inducible factor 2-alpha
 UniProt Gene Name    Epas1 [Similar Products]
 UniProt Synonym Gene Names    Hif2a; EPAS-1; HLF; mHLF; HRF; HIF-2-alpha; HIF2-alpha [Similar Products]
 UniProt Entry Name    EPAS1_MOUSE
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 UniProt Comments for HIF2a    HIF2A: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP. Interacts with EGLN1. Interacts with VHL. Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Protein type: Transcription factor; DNA-binding

Cellular Component: transcription factor complex; cytoplasm; nuclear speck; nucleus

Molecular Function: protein dimerization activity; histone acetyltransferase binding; signal transducer activity; protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; myoblast cell fate commitment; embryonic placenta development; transcription, DNA-dependent; regulation of heart rate; multicellular organismal development; cell maturation; norepinephrine metabolic process; signal transduction; iron ion homeostasis; regulation of transcription, DNA-dependent; visual perception; response to hypoxia; erythrocyte differentiation; blood vessel remodeling; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; cell differentiation; surfactant homeostasis; lung development
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 Research Articles on HIF2a    1. HIF-2alpha target genes are upregulated in sclerosing glomeruli.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HIF2a recombinant proteinPathways associated with HIF2a recombinant protein
 Reference Product  PubMed Publications
 Egln1 recombinant protein  >14 publications with HIF2a and Egln1
 Vhl recombinant protein  >12 publications with HIF2a and Vhl
 Arnt recombinant protein  >9 publications with HIF2a and Arnt
 Vegfa recombinant protein  >7 publications with HIF2a and Vegfa
 Epo recombinant protein  >4 publications with HIF2a and Epo
 Car9 recombinant protein  >1 publications with HIF2a and Car9
 Egln3 recombinant protein  >1 publications with HIF2a and Egln3
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
 Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies  PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
 Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway antibodies  Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway Diagram
 Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway antibodies  Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway Diagram
 Renal Cell Carcinoma Pathway antibodies  Renal Cell Carcinoma Pathway Diagram
Diseases associated with HIF2a recombinant proteinOrgans/Tissues associated with HIF2a recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >29 publications with HIF2a and Neoplasms
 Carcinoma, Renal Cell Antibodies  >9 publications with HIF2a and Carcinoma, Renal Cell
 Disease Models, Animal Antibodies  >8 publications with HIF2a and Disease Models, Animal
 Necrosis Antibodies  >6 publications with HIF2a and Necrosis
 Cardiovascular Diseases Antibodies  >6 publications with HIF2a and Cardiovascular Diseases
 Kidney Diseases Antibodies  >5 publications with HIF2a and Kidney Diseases
 Inflammation Antibodies  >5 publications with HIF2a and Inflammation
 Hypertrophy Antibodies  >4 publications with HIF2a and Hypertrophy
 Liver Diseases Antibodies  >3 publications with HIF2a and Liver Diseases
 Liver Neoplasms Antibodies  >3 publications with HIF2a and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >25 publications with HIF2a and Blood
 Liver Antibodies  >13 publications with HIF2a and Liver
 Kidney Antibodies  >13 publications with HIF2a and Kidney
 Heart Antibodies  >10 publications with HIF2a and Heart
 Muscle Antibodies  >10 publications with HIF2a and Muscle
 Lung Antibodies  >9 publications with HIF2a and Lung
 Embryonic Tissue Antibodies  >9 publications with HIF2a and Embryonic Tissue
 Brain Antibodies  >6 publications with HIF2a and Brain
 Adrenal Gland Antibodies  >4 publications with HIF2a and Adrenal Gland
 Thyroid Antibodies  >2 publications with HIF2a and Thyroid
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