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IL35 recombinant protein :: IL-35 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS553262 IL35 recombinant protein
Unit / Price
0.01 mg  /  $510 +1 FREE 8GB USB
0.05 mg  /  $1,915 +2 FREE 8GB USB
 Product Name   

IL-35 (IL35), Recombinant Protein

★Popular Item★
 Also Known As   

Human IL-35 Recombinant

 Product Synonym Names    il-35; il35
 Product Gene Name   

IL35 recombinant protein

[Similar Products]
 Product Synonym Gene Name    IL-35 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    605816
 3D Structure    ModBase 3D Structure for Q14213
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 Host    HEK Cells
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 Purity/Purification    >95%, as determined by SDS-PAGE
 Form/Format    Recombinant Human Interleukin-35 was lyophilized from a 0.2 um filtered PBS solution pH 7.4.
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 Host Note    DNA sequence encoding Human IL27B fused to DNA sequence encoding mature chain of Human IL12 p35 via linker chain and a C-terminal polyHis tag was expressed in HEK cells.
 Endotoxin    Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
 Reconstitution    A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
 Molecular Weight Note    Recombinant Human Interleukin-35 is a monomer consisting of 645 amino acid residue, and migrates as an approximately 95 kDa protein under reducing conditions in SDS-PAGE.
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 Preparation and Storage    The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
 Other Notes    Small volumes of IL35 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for IL35 recombinant protein    Cytokines
NCBI/Uniprot data below describe general gene information for IL35. It may not necessarily be applicable to this product.
 NCBI GI #    14577917
 NCBI GeneID    10148
 NCBI Accession #    NP_005746.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_005755.2 [Other Products]
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 UniProt Primary Accession #    Q14213 [Other Products]
 UniProt Secondary Accession #    O75269; A0N0N2 [Other Products]
 Molecular Weight    25,396 Da
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 NCBI Official Full Name    interleukin-27 subunit beta
 UniProt Protein Name    Interleukin-27 subunit beta
 UniProt Synonym Protein Names   
Epstein-Barr virus-induced gene 3 protein
 UniProt Gene Name    EBI3 [Similar Products]
 UniProt Synonym Gene Names    IL27B; IL-27 subunit beta; IL-27B [Similar Products]
 UniProt Entry Name    IL27B_HUMAN
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 NCBI Summary for IL35    This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008]
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 UniProt Comments for IL35    IL27-beta: Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. Belongs to the type I cytokine receptor family. Type 3 subfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; interleukin-27 receptor binding; cytokine activity

Biological Process: T-helper 1 type immune response; cytokine and chemokine mediated signaling pathway; positive regulation of alpha-beta T cell proliferation; humoral immune response; positive regulation of interferon-gamma biosynthetic process
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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