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| Product Name
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IMPDH2, Recombinant Protein
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| Also Known As
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IMPDH2, 1-514aa, Human, His tag, E Coli
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| Product Synonym Names
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Inosine-5'-monophosphate dehydrogenase 2; IMPD2; IMPDH-II
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Sequence
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MGSSHHHHHH SSGLVPRGSH MADYLISGGT SYVPDDGLTA QQLFNCGDGL TYNDFLILPG YIDFTADQVD LTSALTKKIT LKTPLVSSPM DTVTEAGMAI AMALTGGIGF IHHNCTPEFQ ANEVRKVKKY EQGFITDPVV LSPKDRVRDV FEAKARHGFC GIPITDTGRM GSRLVGIISS RDIDFLKEEE HDCFLEEIMT KREDLVVAPA GITLKEANEI LQRSKKGKLP IVNEDDELVA IIARTDLKKN RDYPLASKDA KKQLLCGAAI GTHEDDKYRL DLLAQAGVDV VVLDSSQGNS IFQINMIKYI KDKYPNLQVI GGNVVTAAQA KNLIDAGVDA LRVGMGSGSI CITQEVLACG RPQATAVYKV SEYARRFGVP VIADGGIQNV GHIAKALALG ASTVMMGSLL AATTEAPGEY FFSDGIRLKK YRGMGSLDAM DKHLSSQNRY FSEADKIKVA QGVSGAVQDK GSIHKFVPYL IAGIQHSCQD IGAKSLTQVR AMMYSGELKF EKRTSSAQVE GGVHSLHSYE KRLF
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| OMIM
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J04208 mRNA
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| 3D Structure
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ModBase 3D Structure for P12268
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| Host
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E Coli
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| Purity/Purification
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> 90% by SDS-PAGE
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| Form/Format
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Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150mM NaCl
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| Concentration
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0.5 mg/ml (determined by Bradford assay) (lot specific)
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| Antigen Species
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Human
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| Tag Information
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His-tag
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| Preparation and Storage
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Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
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| Other Notes
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Small volumes of IMPDH2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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IMPDH2 belongs to the IMPDH/GMPR family. It catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. IMPDH2 is the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. Also IMPDH1 and IMPDH2 are targets for the important immunosuppressive drug, mycophenolic acid (MPA). Recombinant human IMPDH2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
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| Product Categories/Family for IMPDH2 recombinant protein
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Metabolism
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| Applications Tested/Suitable for IMPDH2 recombinant protein
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SDS-PAGE
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| SDS-Page of IMPDH2 recombinant protein
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TOP
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| NCBI/Uniprot data below describe general gene information for IMPDH2. It may not necessarily be applicable to this product. |
| NCBI GI #
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66933016
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| NCBI GeneID
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3615
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| NCBI Accession #
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NP_000875
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_000884.2
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[Other Products]
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| UniProt Primary Accession #
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P12268
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[Other Products]
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| UniProt Secondary Accession #
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Q6LEF3
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[Other Products]
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| UniProt Related Accession #
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P12268
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[Other Products]
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| Molecular Weight
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58.0kDa (534aa)
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TOP
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| NCBI Official Full Name
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inosine-5'-monophosphate dehydrogenase 2
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| NCBI Official Synonym Full Names
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inosine monophosphate dehydrogenase 2
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| NCBI Official Symbol
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IMPDH2
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[Similar Products]
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| NCBI Official Synonym Symbols
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IMPD2; IMPDH-II
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[Similar Products]
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| NCBI Protein Information
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inosine-5'-monophosphate dehydrogenase 2
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| UniProt Protein Name
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Inosine-5'-monophosphate dehydrogenase 2
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| UniProt Synonym Protein Names
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IMPDH-II
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| UniProt Gene Name
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IMPDH2
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[Similar Products]
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| UniProt Synonym Gene Names
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IMP dehydrogenase 2; IMPD 2; IMPDH 2
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[Similar Products]
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| NCBI Summary for IMPDH2
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This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq, Jul 2008]
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| UniProt Comments for IMPDH2
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Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
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TOP
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Wu TY, Peng Y, et al. (2010) Pharmacol. 161 (7):1584-98. ; Bremer S, et al. (2008) Transplantation. 85 (1):55-61.
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| Research Articles on IMPDH2
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1. inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) is an intracellular target of the PPIA-Sanglifehrin A binary complex.
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with IMPDH2 recombinant protein | Pathways associated with IMPDH2 recombinant protein |
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| Diseases associated with IMPDH2 recombinant protein | Organs/Tissues associated with IMPDH2 recombinant protein |
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TOP
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Recombinant Protein 
