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IRS1 recombinant protein

Scan QR to view Datasheet Catalog #    MBS515371
SDS-PAGE
Unit / Price
0.02 mg  /  $260 +1 FREE 8GB USB
0.05 mg  /  $415 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IRS1, Recombinant Protein

★Popular Item★
 Also Known As   

IRS1 recombinant protein

 Product Synonym Names    Insulin Receptor Substrate 1; HIRS-1
 Product Gene Name   

IRS1 recombinant protein

[Similar Products]
 Product Synonym Gene Name    HIRS-1 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    600-1245
 Chromosome Location    Chromosome: 2; NC_000002.11 (227596033..227664545, complement). Location: 2q36
 OMIM    125853
 3D Structure    ModBase 3D Structure for P35568
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 Host    Sf9 insect cells
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 Form/Format    50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
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 Type    Recombinant Fusion Protein
 Accession number    NM 005544
 Species    Human
 Tag Information    GST tag
 Expression System    Sf9 insect cells using baculovirus
 Source Note    Recombinant human IRS1 (600-1245) was expressed by baculovirus in Sf9 insect cells
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 Preparation and Storage    Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
 Other Notes    Small volumes of IRS1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for IRS1 recombinant protein

   Recombinant human IRS1 (600-1245) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: IRS1 is the substrate for the insulin tyrosine kinase receptor and is found in a variety of insulin-responsive cells and tissues. IRS1 protein has no intrinsic enzymatic activity but acts as a docking protein, via the SH2 domains, for mediating the insulin downstream signaling events. IRS1 has been shown to associate with the 14-3-3 family of proteins and this could play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS1 (1). IRS1 may be associated with colorectal cancer and diet and related factors may affect the risk by modifying plasma insulin levels. Thus, the inter-individual variation in insulin signaling mediated by IRS1 may play a plausible role in the development of colorectal cancer (2).
 Product Categories/Family for IRS1 recombinant protein    Proteins Substrates; Signaling Reagents - Protein Substrates
 Applications Tested/Suitable for IRS1 recombinant protein   

Kinase Assay, Western Blot (WB)

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 SDS-PAGE of IRS1 recombinant protein    The purity of IRS 1 was determined to be >8O% by densitometry.
Approx. MW 118kDa.
IRS1 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for IRS1. It may not necessarily be applicable to this product.
 NCBI GI #    187761322
 NCBI GeneID    3667
 NCBI Accession #    NP_005535.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005544 [Other Products]
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 UniProt Primary Accession #    P35568 [Other Products]
 UniProt Related Accession #    P35568 [Other Products]
 Molecular Weight    ~118 kDa
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 NCBI Official Full Name    Homo sapiens insulin receptor substrate 1 (IRS1), mRNA
 NCBI Official Synonym Full Names    insulin receptor substrate 1
 NCBI Official Symbol    IRS1 [Similar Products]
 NCBI Official Synonym Symbols   
HIRS-1
[Similar Products]
 NCBI Protein Information    insulin receptor substrate 1; IRS-1
 UniProt Protein Name    Insulin receptor substrate 1
 Protein Family    Isoleucine--tRNA ligase
 UniProt Gene Name    IRS1 [Similar Products]
 UniProt Synonym Gene Names    IRS-1 [Similar Products]
 UniProt Entry Name    IRS1_HUMAN
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 NCBI Summary for IRS1    This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
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 UniProt Comments for IRS1    Function: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit

By similarity. Ref.14 Ref.30

Subunit structure: Interacts with UBTF and PIK3CA

By similarity. Interacts (via phosphorylated YXXM motifs) with PIK3R1

By similarity. Interacts with ROCK1 and FER

By similarity. Interacts (via PH domain) with PHIP

By similarity. Interacts with GRB2

By similarity. Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif). Interacts with ALK. Interacts with EIF2AK2/PKR

By similarity. Ref.5 Ref.6 Ref.7 Ref.10 Ref.11 Ref.14

Post-translational modification: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor

By similarity. Phosphorylation of Tyr-896 is required for GRB2-binding

By similarity. Phosphorylated by ALK. Phosphorylated at Ser-270, Ser-307, Ser-636 and Ser-1101 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1. Ref.8 Ref.9 Ref.10 Ref.15 Ref.19

Polymorphism: The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependent diabetes mellitus (NIDDM) by producing a cluster of insulin resistance-related metabolic abnormalities. In insulin-stimulated human endothelial cells from carriers of the Arg-971 polymorphism, genetic impairment of the IRS1/PI3K/PDPK1/AKT1 insulin signaling cascade results in impaired insulin-stimulated nitric oxide (NO) release and suggested that this may be a mechanism through which the Arg-971 polymorphism contributes to the genetic predisposition to develop endothelial dysfunction and cardiovascular disease. The Arg-971 polymorphism not only reduces phosphorylation of the substrate but allows IRS1 to act as an inhibitor of PI3K, producing global insulin resistance.

Involvement in disease: Diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]: A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.Note: The gene represented in this entry may be involved in disease pathogenesis. Ref.24 Ref.25 Ref.27 Ref.30 Ref.31

Sequence similarities: Contains 1 IRS-type PTB domain.Contains 1 PH domain.
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Product References and Citations for IRS1 recombinant protein

   1. Ogihara, T. et al: 14-3-3 protein binds to insulin receptor substrate-1, one of the binding sites of which is in the phosphotyrosine binding domain. J. Biol. Chem. 272: 25267-25274, 1997.
2. Slattery, M.L. et al: Genetic variation in IGF1, IGFBP3, IRS1, IRS2 and risk of breast cancer in women living in outhwestern United States. Breast Cancer Res Treat. 2007.
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 Research Articles on IRS1    1. Data indicate the association of Par14 with insulin receptor substrate 1 (IRS-1) in human HepG2 cells overexpressing both as well as endogenously in the mouse liver.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IRS1 recombinant proteinPathways associated with IRS1 recombinant protein
 Reference Product  PubMed Publications
 IGF1R recombinant protein  >227 publications with IRS1 and IGF1R
 GRB2 recombinant protein  >137 publications with IRS1 and GRB2
 JAK2 recombinant protein  >59 publications with IRS1 and JAK2
 PTPN11 recombinant protein  >41 publications with IRS1 and PTPN11
 INSR recombinant protein  >39 publications with IRS1 and INSR
 JAK1 recombinant protein  >26 publications with IRS1 and JAK1
 MAPK8 recombinant protein  >22 publications with IRS1 and MAPK8
 PIK3R1 recombinant protein  >12 publications with IRS1 and PIK3R1
 PIK3CA recombinant protein  >5 publications with IRS1 and PIK3CA
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
Diseases associated with IRS1 recombinant proteinOrgans/Tissues associated with IRS1 recombinant protein
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >1131 publications with IRS1 and Insulin Resistance
 Hyperinsulinism Antibodies  >765 publications with IRS1 and Hyperinsulinism
 Diabetes Mellitus Antibodies  >454 publications with IRS1 and Diabetes Mellitus
 Neoplasms Antibodies  >358 publications with IRS1 and Neoplasms
 Diabetes Mellitus, Type 2 Antibodies  >316 publications with IRS1 and Diabetes Mellitus, Type 2
 Necrosis Antibodies  >164 publications with IRS1 and Necrosis
 Hyperglycemia Antibodies  >161 publications with IRS1 and Hyperglycemia
 Liver Diseases Antibodies  >120 publications with IRS1 and Liver Diseases
 Inflammation Antibodies  >113 publications with IRS1 and Inflammation
 Cardiovascular Diseases Antibodies  >102 publications with IRS1 and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >811 publications with IRS1 and Muscle
 Liver Antibodies  >448 publications with IRS1 and Liver
 Connective Tissue Antibodies  >227 publications with IRS1 and Connective Tissue
 Pancreas Antibodies  >136 publications with IRS1 and Pancreas
 Brain Antibodies  >113 publications with IRS1 and Brain
 Heart Antibodies  >112 publications with IRS1 and Heart
 Ovary Antibodies  >106 publications with IRS1 and Ovary
 Kidney Antibodies  >93 publications with IRS1 and Kidney
 Nerve Antibodies  >43 publications with IRS1 and Nerve
 Bone Antibodies  >41 publications with IRS1 and Bone
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