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ITGa2 recombinant protein :: Integrin Alpha 2 (ITGa2) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2009245 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $115 +1 FREE 8GB USB
0.05 mg  /  $200 +1 FREE 8GB USB
0.1 mg  /  $290 +1 FREE 8GB USB
0.2 mg  /  $360 +1 FREE 8GB USB
0.5 mg  /  $690 +1 FREE 8GB USB
1 mg  /  $1,015 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Integrin Alpha 2 (ITGa2), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Integrin Alpha 2 (ITGa2)

 Product Gene Name   

ITGa2 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
The sequence of the target protein is listed below. EN RMGDV YKCPVDLSTA TCEKLNLQTS TSIPNVTEMK TNMSLGLILT RNMGTGGFLT CGPLWAQQCG NQYYTTGVCS DISPDFQLSA SFSPATQPCP SLIDVVVVCD ESNSIYPWDA VKNFLEKFVQ GLDIGPTKTQ VGLIQYANNP RVVFNLNTYK TKEEMIVATS QTSQYGGDLT NTFGAIQYAR KYAYSAASGG RRSATKV
 Chromosome Location    Chromosome: 5; NC_000005.9 (52285156..52390609). Location: 5q11.2
 OMIM    192974
 3D Structure    ModBase 3D Structure for P17301
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
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 Organism    Homo sapiens (Human)
 Expression System    Prokaryotic expression
 Residues    Glu74~Val277 (Accession # P17301) with two N-terminal Tags, His-tag and S-tag
 Subcellular Location    Membrane; Single-pass type I membrane protein.
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Predicted Isoelectric Point    5.8
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
 Accurate Molecular Mass    33kDa as determined by SDS-PAGE reducing conditions.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of ITGa2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ITGa2 recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for ITGa2 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of ITGa2 recombinant protein    ITGa2 recombinant protein SDS-PAGE image
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 SDS-PAGE of ITGa2 recombinant protein    [ DESCRIPTION]
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa.
Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SDS-polyacrylamide gel.
[ LOADING VOLUMES]
3~5uL per well for mini gel; 7uL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
ITGa2 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for ITGa2. It may not necessarily be applicable to this product.
 NCBI GI #    116295258
 NCBI GeneID    3673
 NCBI Accession #    NP_002194.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002203.3 [Other Products]
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 UniProt Primary Accession #    P17301 [Other Products]
 UniProt Secondary Accession #    Q14595 [Other Products]
 UniProt Related Accession #    P17301 [Other Products]
 Molecular Weight    27.9kDa
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 NCBI Official Full Name    integrin alpha-2
 NCBI Official Synonym Full Names    integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
 NCBI Official Symbol    ITGA2 [Similar Products]
 NCBI Official Synonym Symbols   
BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
[Similar Products]
 NCBI Protein Information    integrin alpha-2; collagen receptor; platelet antigen Br; platelet glycoprotein GPIa; VLA2 receptor, alpha-2 subunit; CD49 antigen-like family member B; platelet membrane glycoprotein Ia; human platelet alloantigen system 5; very late activation protein 2 receptor, alpha-2 subunit
 UniProt Protein Name    Integrin alpha-2
 UniProt Synonym Protein Names   
CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha
 Protein Family    Integrin
 UniProt Gene Name    ITGA2 [Similar Products]
 UniProt Synonym Gene Names    CD49B; GPIa [Similar Products]
 UniProt Entry Name    ITA2_HUMAN
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 NCBI Summary for ITGa2    This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
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 UniProt Comments for ITGa2    ITGA2: Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Belongs to the integrin alpha chain family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cell surface; focal adhesion; perinuclear region of cytoplasm; plasma membrane; nerve terminal; nucleus; integrin complex; external side of plasma membrane

Molecular Function: viral receptor activity; collagen binding; integrin binding; protein binding; protein heterodimerization activity; metal ion binding; laminin binding

Biological Process: axon guidance; entry of virus into host cell; extracellular matrix organization and biogenesis; response to muscle activity; positive regulation of positive chemotaxis; positive regulation of cell adhesion; positive regulation of translation; cell-matrix adhesion; positive regulation of leukocyte migration; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of collagen binding; positive regulation of cell projection organization and biogenesis; mesodermal cell differentiation; positive regulation of smooth muscle cell migration; response to L-ascorbic acid; response to organic cyclic substance; establishment of protein localization; mammary gland development; positive regulation of smooth muscle contraction; cell adhesion; positive regulation of DNA binding; substrate-bound cell migration; response to drug; integrin-mediated signaling pathway; skin morphogenesis; hypotonic response; cell-substrate adhesion; cellular response to hormone stimulus; detection of mechanical stimulus involved in sensory perception of pain; cell proliferation; organ morphogenesis; response to hypoxia; cell adhesion mediated by integrin; blood coagulation; positive regulation of phagocytosis, engulfment; response to amine stimulus; positive regulation of transmission of nerve impulse; positive regulation of inflammatory response

Disease: Bleeding Disorder, Platelet-type, 9
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 Research Articles on ITGa2    1. HPA-5 typing discrepancy reveals an Ile503Leu substitution in platelet GPIa (alpha2 integrin).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ITGa2 recombinant proteinPathways associated with ITGa2 recombinant protein
 Reference Product  PubMed Publications
 ITGB3 recombinant protein  >24 publications with ITGa2 and ITGB3
 ITGB1 recombinant protein  >8 publications with ITGa2 and ITGB1
 COL3A1 recombinant protein  >1 publications with ITGa2 and COL3A1
 FN1 recombinant protein  >1 publications with ITGa2 and FN1
 COL1A2 recombinant protein  >1 publications with ITGa2 and COL1A2
 COL1A1 recombinant protein  >1 publications with ITGa2 and COL1A1
 Products by Pathway  Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 CHL1 Interactions Pathway antibodies  CHL1 Interactions Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
Diseases associated with ITGa2 recombinant proteinOrgans/Tissues associated with ITGa2 recombinant protein
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >25 publications with ITGa2 and Cardiovascular Diseases
 Neoplasms Antibodies  >13 publications with ITGa2 and Neoplasms
 Nervous System Diseases Antibodies  >11 publications with ITGa2 and Nervous System Diseases
 Heart Diseases Antibodies  >7 publications with ITGa2 and Heart Diseases
 Inflammation Antibodies  >5 publications with ITGa2 and Inflammation
 Fibrosis Antibodies  >4 publications with ITGa2 and Fibrosis
 Hypertension Antibodies  >4 publications with ITGa2 and Hypertension
 Congenital Abnormalities Antibodies  >4 publications with ITGa2 and Congenital Abnormalities
 Kidney Diseases Antibodies  >3 publications with ITGa2 and Kidney Diseases
 Breast Neoplasms Antibodies  >2 publications with ITGa2 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >32 publications with ITGa2 and Blood
 Vascular Antibodies  >8 publications with ITGa2 and Vascular
 Brain Antibodies  >5 publications with ITGa2 and Brain
 Kidney Antibodies  >4 publications with ITGa2 and Kidney
 Bone Antibodies  >4 publications with ITGa2 and Bone
 Placenta Antibodies  >3 publications with ITGa2 and Placenta
 Prostate Antibodies  >3 publications with ITGa2 and Prostate
 Thyroid Antibodies  >2 publications with ITGa2 and Thyroid
 Connective Tissue Antibodies  >2 publications with ITGa2 and Connective Tissue
 Uterus Antibodies  >2 publications with ITGa2 and Uterus
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