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ICA1 recombinant protein :: Islet Cell Autoantigen 1 (ICA1) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2012459 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $155 +1 FREE 8GB USB
0.05 mg  /  $305 +1 FREE 8GB USB
0.1 mg  /  $470 +1 FREE 8GB USB
0.2 mg  /  $580 +1 FREE 8GB USB
0.5 mg  /  $1,125 +1 FREE 8GB USB
1 mg  /  $1,680 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Islet Cell Autoantigen 1 (ICA1), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Islet Cell Autoantigen 1 (ICA1)

 Product Gene Name   

ICA1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-MSGHKCY SWE LQDRFAQDKS VVNKMQQKYW ETKQAFIKAT GKKEDEHVVA SDADLDAKLE LFHSIQRTCL DLSKAIVLYQ KRICFLSQEE NELGKFLRSQ GFQDKTRAGK MMQATGKALC FSSQQRLALR NPLCRFHQEV ETFRHRAISD TWLTVNRMEQ YRTEYRGALL WMKDVSQELD PDLYKQMEKF RKVQTQVRLA KKNFDKLKMD VCQKVDLLGA SRCNLLSHML ATYQTTLLHF WEKTSHTMAA IHESFKG
 Chromosome Location    Chromosome: 6; NC_000072.6 (8630527..8778542, complement). Location: 6 A1-A2; 6 4.38 cM
 3D Structure    ModBase 3D Structure for P97411
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    Met1~Gly257 (Accession # P97411) with two N-terminal Tags, His-tag and S-tag
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
 Predicted isoelectric point    8.8
 Predicted Molecular Mass    35.9kDa as determined by SDS-PAGE reducing conditions.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of ICA1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ICA1 recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for ICA1 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
(May be suitable for use in other assays to be determined by the end user.)

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 SDS-PAGE of ICA1 recombinant protein    ICA1 recombinant protein SDS-PAGE image
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 SDS-PAGE of ICA1 recombinant protein    [ DESCRIPTION]
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa.
Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SDS-polyacrylamide gel.
[ LOADING VOLUMES]
3~5uL per well for mini gel; 7uL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by Dn oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
ICA1 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for ICA1. It may not necessarily be applicable to this product.
 NCBI GI #    356582438
 NCBI GeneID    15893
 NCBI Accession #    NP_001239195.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001252266.1 [Other Products]
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 UniProt Primary Accession #    P97411 [Other Products]
 UniProt Secondary Accession #    P97362; P97954; Q99M40; Q9R2C4; E9QLS3 [Other Products]
 UniProt Related Accession #    P97411 [Other Products]
 Molecular Weight    26&32&37kDa as determined by SDS-PAGE reducing conditions.
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 NCBI Official Full Name    islet cell autoantigen 1 isoform 2
 NCBI Official Synonym Full Names    islet cell autoantigen 1
 NCBI Official Symbol    Ica1 [Similar Products]
 NCBI Official Synonym Symbols   
69kDa; ICA69
[Similar Products]
 NCBI Protein Information    islet cell autoantigen 1; p69; ICAp69; islet cell autoantigen p69; 69 kDa islet cell autoantigen; islet cell autoantigen 1, 69 kDa
 UniProt Protein Name    Islet cell autoantigen 1
 UniProt Synonym Protein Names   
69 kDa islet cell autoantigen; ICA69; Islet cell autoantigen p69
 Protein Family    Islet cell autoantigen
 UniProt Gene Name    Ica1 [Similar Products]
 UniProt Synonym Gene Names    Icap69; ICA69; ICAp69; p69 [Similar Products]
 UniProt Entry Name    ICA69_MOUSE
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 UniProt Comments for ICA1    ICA1: May play a role in neurotransmitter secretion.

Protein type: Unknown function

Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; synaptic vesicle membrane; membrane; perinuclear region of cytoplasm; secretory granule membrane; dendrite; cytoplasm; synapse; cytoplasmic vesicle; cell junction; cytosol

Molecular Function: protein domain specific binding

Biological Process: transport; neurotransmitter transport; regulation of protein homodimerization activity; regulation of neurotransmitter secretion
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 Research Articles on ICA1    1. PICK1 and ICA69 are key regulators of the formation and maturation of insulin granules.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ICA1 recombinant proteinDiseases associated with ICA1 recombinant protein
 Products by Pathway  Pathway Diagram
 Type I Diabetes Mellitus Pathway antibodies  Type I Diabetes Mellitus Pathway Diagram
 Type I Diabetes Mellitus Pathway antibodies  Type I Diabetes Mellitus Pathway Diagram
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >9 publications with ICA1 and Disease Models, Animal
 Adenoma Antibodies  >3 publications with ICA1 and Adenoma
 Inflammation Antibodies  >2 publications with ICA1 and Inflammation
 Heart Diseases Antibodies  >1 publications with ICA1 and Heart Diseases
 Weight Loss Antibodies  >1 publications with ICA1 and Weight Loss
 Hyperplasia Antibodies  >1 publications with ICA1 and Hyperplasia
 Necrosis Antibodies  >1 publications with ICA1 and Necrosis
Organs/Tissues associated with ICA1 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >36 publications with ICA1 and Blood
 Pancreas Antibodies  >22 publications with ICA1 and Pancreas
 Brain Antibodies  >18 publications with ICA1 and Brain
 Heart Antibodies  >3 publications with ICA1 and Heart
 Thymus Antibodies  >3 publications with ICA1 and Thymus
 Testis Antibodies  >2 publications with ICA1 and Testis
 Salivary Gland Antibodies  >2 publications with ICA1 and Salivary Gland
 Embryonic Tissue Antibodies  >2 publications with ICA1 and Embryonic Tissue
 Lung Antibodies  >1 publications with ICA1 and Lung
 Liver Antibodies  >1 publications with ICA1 and Liver
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