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PKAR-1a recombinant protein :: Kinase A regulatory subunit-1 alpha Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS142748 PKAR-1a recombinant protein
Unit / Price
0.005 mg  /  $375 +1 FREE 8GB USB
0.025 mg  /  $930 +1 FREE 8GB USB
0.05 mg  /  $1,540 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Kinase A regulatory subunit-1 alpha (PKAR-1a), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human Protein Kinase A regulatory subunit-1 alpha

 Product Synonym Names    PKAR-I alpha Human; Protein Kinase A regulatory subunit-1 alpha Human Recombinant; cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1; PRKAR1A PKR1; PRKAR1; CAR; CNC; CNC1; PKR1; ADOHR; PPNAD1; ACRDYS1
 Product Gene Name   

PKAR-1a recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    101800
 3D Structure    ModBase 3D Structure for P10644
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 Host    E Coli
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 Purity/Purification    Greater than 95% as determined by SDS-PAGE.
 Form/Format    PKA regulatory subunit-I alpha is supplied at a concentration of 1.3 mg/ml in 20mM MOPS (pH 7.0), 150mM NaCl, 1mM 2-mercaptoethanol and 50% glycerol.
Sterile Filtered clear solution.
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 Inhibitory Activity    The cAMP-dependent protein kinase, regulatory subunit RIa reversibly inhibits the catalytic subunit Ca of the cAMP-dependent protein kinase PKA. The inhibition of the catalytic subunit can be reversed by the addition of the second messenger cAMP.
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 Preparation and Storage    PKAR-Ia should be stored at 4 degree C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20 degree C. Avoid multiple freeze-thaw cycles.
 Other Notes    Small volumes of PKAR-1a recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for PKAR-1a recombinant protein

   Description: PKA regulatory subunit I a Human Recombinant is a dimeric 86kDa protein (the monomer is 381 aa 43kDa). PKAR-I alpha is purified by proprietary chromatographic techniques.

Introduction: The Regulatory (R) subunit of Protein Kinase A (PKA) inhibits its kinase activity by shielding the Catalytic (C) subunit from physiological substrates. This inhibition is reversed in response to extra-cellular signals that increase cAMP levels in the cytoplasm. Upon cAMP binding to R, C is allosterically released from R, activating a spectrum of downstream signaling cascades. Crystallographic data indicated that a series of distinct conformational changes within CBD-A must occur to relay the cAMP signal from the cAMP binding site to the R:C interaction interface. One critical cAMP relay site within the CBD-A of R has been identified as Asp170 because the D170A mutation selectively reduces the negative cooperativity between the cAMP- and C-recognition sites (i.e. the KD for the R:C complex in the presence of cAMP is reduced by more than 12-fold), without significantly compromising the high affinity of R for both binding partners.
 Product Categories/Family for PKAR-1a recombinant protein    PROTEIN KINASES; Enzymes; Protein Kinase-A
NCBI/Uniprot data below describe general gene information for PKAR-1a. It may not necessarily be applicable to this product.
 NCBI GI #    443497964
 NCBI GeneID    5573
 NCBI Accession #    NP_001263218.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001276289.1 [Other Products]
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 UniProt Primary Accession #    P10644 [Other Products]
 UniProt Secondary Accession #    Q567S7; K7ER48 [Other Products]
 UniProt Related Accession #    P10644 [Other Products]
 Molecular Weight    38,003 Da
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 NCBI Official Full Name    cAMP-dependent protein kinase type I-alpha regulatory subunit isoform a
 NCBI Official Synonym Full Names    protein kinase, cAMP-dependent, regulatory, type I, alpha
 NCBI Official Symbol    PRKAR1A [Similar Products]
 NCBI Official Synonym Symbols   
CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
[Similar Products]
 NCBI Protein Information    cAMP-dependent protein kinase type I-alpha regulatory subunit; Carney complex type 1; cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; protein kinase A type 1a regulatory subunit; tissue-specific extinguisher 1
 UniProt Protein Name    cAMP-dependent protein kinase type I-alpha regulatory subunit
 UniProt Synonym Protein Names   
Tissue-specific extinguisher 1; TSE1
 UniProt Gene Name    PRKAR1A [Similar Products]
 UniProt Synonym Gene Names    PKR1; PRKAR1; TSE1; TSE1 [Similar Products]
 UniProt Entry Name    KAP0_HUMAN
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 NCBI Summary for PKAR-1a    cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed. [provided by RefSeq, Jan 2013]
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 UniProt Comments for PKAR-1a    PKAR1A: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Interacts with RFC2: the complex may be involved in cell survival. Defects in PRKAR1A are the cause of Carney complex type 1 (CNC1). CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: protein complex; membrane; plasma membrane; cytosol; neuromuscular junction; AMP-activated protein kinase complex; cAMP-dependent protein kinase complex

Molecular Function: protein binding; ubiquitin protein ligase binding; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; female meiosis; cardiac muscle cell proliferation; sarcomere organization; signal transduction; regulation of transcription from RNA polymerase II promoter; mesoderm formation; phospholipase C activation; negative regulation of meiosis; energy reserve metabolic process; innate immune response; renal water homeostasis; blood coagulation; regulation of insulin secretion; transmembrane transport

Disease: Myxoma, Intracardiac; Carney Complex, Type 1; Thyroid Carcinoma, Papillary; Acrodysostosis 1 With Or Without Hormone Resistance; Pigmented Nodular Adrenocortical Disease, Primary, 1
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 Research Articles on PKAR-1a    1. Results show that mouse Prkar1a and human PRKAR2A exhibited a dynamic spatio-temporal expression in tooth development, whereas neither human PRKAR1A nor mouse Prkar2a showed their expression in odontogenesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PKAR-1a recombinant proteinPathways associated with PKAR-1a recombinant protein
 Reference Product  PubMed Publications
 GNAS recombinant protein  >26 publications with PKAR-1a and GNAS
 PRKAR1B recombinant protein  >15 publications with PKAR-1a and PRKAR1B
 PRKACA recombinant protein  >8 publications with PKAR-1a and PRKACA
 PRKACB recombinant protein  >5 publications with PKAR-1a and PRKACB
 AKAP1 recombinant protein  >4 publications with PKAR-1a and AKAP1
 ADCY9 recombinant protein  >1 publications with PKAR-1a and ADCY9
 ADCY2 recombinant protein  >1 publications with PKAR-1a and ADCY2
 PRKACG recombinant protein  >1 publications with PKAR-1a and PRKACG
 AKAP10 recombinant protein  >1 publications with PKAR-1a and AKAP10
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Aquaporin-mediated Transport Pathway antibodies  Aquaporin-mediated Transport Pathway Diagram
 Ca-dependent Events Pathway antibodies  Ca-dependent Events Pathway Diagram
 CaM Pathway antibodies  CaM Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calmodulin Induced Events Pathway antibodies  Calmodulin Induced Events Pathway Diagram
 DAG And IP3 Signaling Pathway antibodies  DAG And IP3 Signaling Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
Diseases associated with PKAR-1a recombinant proteinOrgans/Tissues associated with PKAR-1a recombinant protein
 Disease Name  Pubmed Publications
 Carney Complex Antibodies  >188 publications with PKAR-1a and Carney Complex
 Cardiovascular Diseases Antibodies  >68 publications with PKAR-1a and Cardiovascular Diseases
 Heart Diseases Antibodies  >65 publications with PKAR-1a and Heart Diseases
 Hyperplasia Antibodies  >54 publications with PKAR-1a and Hyperplasia
 Cushing Syndrome Antibodies  >51 publications with PKAR-1a and Cushing Syndrome
 Disease Models, Animal Antibodies  >28 publications with PKAR-1a and Disease Models, Animal
 Liver Neoplasms Antibodies  >10 publications with PKAR-1a and Liver Neoplasms
 Adrenal Hyperplasia, Congenital Antibodies  >6 publications with PKAR-1a and Adrenal Hyperplasia, Congenital
 Liver Neoplasms, Experimental Antibodies  >4 publications with PKAR-1a and Liver Neoplasms, Experimental
 Pancreatic Neoplasms Antibodies  >4 publications with PKAR-1a and Pancreatic Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >66 publications with PKAR-1a and Skin
 Adrenal Gland Antibodies  >60 publications with PKAR-1a and Adrenal Gland
 Blood Antibodies  >50 publications with PKAR-1a and Blood
 Heart Antibodies  >43 publications with PKAR-1a and Heart
 Thyroid Antibodies  >41 publications with PKAR-1a and Thyroid
 Brain Antibodies  >23 publications with PKAR-1a and Brain
 Bone Antibodies  >21 publications with PKAR-1a and Bone
 Pituitary Gland Antibodies  >20 publications with PKAR-1a and Pituitary Gland
 Embryonic Tissue Antibodies  >16 publications with PKAR-1a and Embryonic Tissue
 Muscle Antibodies  >16 publications with PKAR-1a and Muscle
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