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KL recombinant protein :: Klotho (KL) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2010008 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $135 +1 FREE 8GB USB
0.05 mg  /  $245 +1 FREE 8GB USB
0.1 mg  /  $365 +1 FREE 8GB USB
0.2 mg  /  $450 +1 FREE 8GB USB
0.5 mg  /  $870 +1 FREE 8GB USB
1 mg  /  $1,280 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Klotho (KL), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Klotho (KL)

 Product Gene Name   

KL recombinant protein

[Similar Products]
 Matching Pairs    Antibody: Klotho (KL) (MBS2003676)
Antigen: Klotho (KL) (MBS2010008)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 MBS2010008 Technical Datasheet    Technical Datasheet PDF
 Chromosome Location    Chromosome: 5; NC_000071.6 (150952607..150993817). Location: 5; 5 G3
 3D Structure    ModBase 3D Structure for O35082
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 Host    E Coli
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 Purity/Purification    > 90%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
 Concentration    Original Concentration: 200 Ug/mL (lot specific)
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 Organism Species    Mus musculus(Mouse)
 Source    Prokaryotic expression
 Residues    Leu59~Phe508
 Tags    N-terminal His Tag
 Subcellular Location    Membrane, Secreted
 Traits    Freeze-dried powder
 Buffer formulation    20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
 Predicted isoelectric point    8.3
 Predicted Molecular Mass    52.7kDa
 Accurate Molecular Mass    53kDa as determined by SDS-PAGE reducing conditions
 USAGE    Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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 Preparation and Storage    Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8C for one month.
Aliquot and store at -80C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of KL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for KL recombinant protein   

Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

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 Sequence of KL recombinant protein    KL recombinant protein Sequence image
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 SDS-PAGE of KL recombinant protein    KL recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for KL. It may not necessarily be applicable to this product.
 NCBI GI #    227330584
 NCBI GeneID    16591
 NCBI Accession #    NP_038851.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013823.2 [Other Products]
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 UniProt Primary Accession #    O35082 [Other Products]
 UniProt Secondary Accession #    O70175; O70621; B2RR78 [Other Products]
 UniProt Related Accession #    O35082 [Other Products]
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 NCBI Official Full Name    klotho
 NCBI Official Synonym Full Names    klotho
 NCBI Official Symbol    Kl [Similar Products]
 NCBI Official Synonym Symbols   
alpha-kl
[Similar Products]
 NCBI Protein Information    klotho; secreted form of Klotho protein
 UniProt Protein Name    Klotho
 Protein Family    Klotho
 UniProt Gene Name    Kl [Similar Products]
 UniProt Entry Name    KLOT_MOUSE
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 UniProt Comments for KL    Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Hydrolase; EC 3.2.1.31

Cellular Component: extracellular space; membrane; extracellular region; plasma membrane; integral to membrane

Molecular Function: signal transducer activity; protein binding; beta-glucuronidase activity; fibroblast growth factor binding; hydrolase activity; hydrolase activity, acting on glycosyl bonds; hydrolase activity, hydrolyzing O-glycosyl compounds; fibroblast growth factor receptor binding

Biological Process: calcium ion homeostasis; fibroblast growth factor receptor signaling pathway; metabolic process; carbohydrate metabolic process; energy reserve metabolic process; insulin receptor signaling pathway; positive regulation of bone mineralization; acute inflammatory response; aging
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 Research Articles on KL    1. Klotho is expressed in the retina and is important for healthy retinal function. Although the mechanisms for the observed abnormalities are not known, they are consistent with the accelerating aging phenotype seen in other tissues.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KL recombinant proteinPathways associated with KL recombinant protein
 Reference Product  PubMed Publications
 Fgf23 recombinant protein  >30 publications with KL and Fgf23
 Fgf15 recombinant protein  >1 publications with KL and Fgf15
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
Diseases associated with KL recombinant proteinOrgans/Tissues associated with KL recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >294 publications with KL and Neoplasms
 Skin Diseases Antibodies  >113 publications with KL and Skin Diseases
 Pulmonary Fibrosis Antibodies  >112 publications with KL and Pulmonary Fibrosis
 Inflammation Antibodies  >79 publications with KL and Inflammation
 Heart Diseases Antibodies  >71 publications with KL and Heart Diseases
 Death Antibodies  >66 publications with KL and Death
 Lung Injury Antibodies  >58 publications with KL and Lung Injury
 Kidney Diseases Antibodies  >53 publications with KL and Kidney Diseases
 Necrosis Antibodies  >40 publications with KL and Necrosis
 Hypertension Antibodies  >34 publications with KL and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >221 publications with KL and Brain
 Liver Antibodies  >121 publications with KL and Liver
 Kidney Antibodies  >119 publications with KL and Kidney
 Testis Antibodies  >30 publications with KL and Testis
 Embryonic Tissue Antibodies  >24 publications with KL and Embryonic Tissue
 Thymus Antibodies  >18 publications with KL and Thymus
 Pancreas Antibodies  >12 publications with KL and Pancreas
 Inner Ear Antibodies  >4 publications with KL and Inner Ear
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