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KLB recombinant protein :: Klotho beta Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS635769 KLB recombinant protein
Unit / Price
0.05 mg  /  $725 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Klotho beta (KLB), Recombinant Protein

★Popular Item★
 Also Known As   

Klotho beta, Recombinant, Human (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein)

 Product Gene Name   

KLB recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 4; NC_000004.11 (39408473..39453153). Location: 4p14
 OMIM    611135
 3D Structure    ModBase 3D Structure for Q86Z14
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 Purity/Purification    Highly Purified
>95% by SDS-PAGE, reducing conditions and visualized with silver stain.
 Form/Format    Supplied as a liquid in PBS, pH 6.8, 0.1mM EDTA, 50% glycerol.
 Concentration    1.06mg/ml (lot specific)
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 Activity    Measured by its binding ability in a functional ELISA. When 2ug/ml (100ul/well) of rhKlotho beta was immobilized onto a Ms x hKlotho beta coated plate, the concentration of rhFGF-21 that produces 50% of the optimal binding response was found to be approximately 3-18ng/ml.
 Endotoxin Level    <1EU/1ug (LAL)
 N-terminal Sequence Analysis    Phe53
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 Preparation and Storage    Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of KLB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for KLB recombinant protein

   Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex. The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin­dependent glucose uptake. The 1043aa type I transmembrane protein is composed of a 51aa signal sequence, a 943aa extracellular domain (ECD) containing two glycosidase-like regions, a 21aa transmembrane domain, and 28aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta-glycosidases, it was initially unclear whether they were functional enzymes. However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible. The extracellular domain shares 79%, 87%, 87% and 67% identity with mouse, equine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.

Murine myeloma cell line, NS0-derived Phe53-Leu997, with a C-terminal 10-His tag.
 Product Categories/Family for KLB recombinant protein    Molecular Biology; MB-Enzymes, Glycosidase
 Application Notes for KLB recombinant protein    SDS-PAGE: 126kD, reducing conditions
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NCBI/Uniprot data below describe general gene information for KLB. It may not necessarily be applicable to this product.
 NCBI GI #    119613331
 NCBI GeneID    152831
 NCBI Accession #    EAW92925.1 [Other Products]
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 UniProt Primary Accession #    Q86Z14 [Other Products]
 UniProt Secondary Accession #    Q2M3K8 [Other Products]
 UniProt Related Accession #    Q86Z14 [Other Products]
 Molecular Weight    110kD [Similar Products]
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 NCBI Official Full Name    klotho beta
 NCBI Official Synonym Full Names    klotho beta
 NCBI Official Symbol    KLB [Similar Products]
 NCBI Official Synonym Symbols   
BKL
[Similar Products]
 NCBI Protein Information    beta-klotho; betaKlotho; klotho beta like; klotho beta-like protein
 UniProt Protein Name    Beta-klotho
 UniProt Synonym Protein Names   
Klotho beta-like protein
 Protein Family    Beta-klotho
 UniProt Gene Name    KLB [Similar Products]
 UniProt Synonym Gene Names    BKL; BetaKlotho [Similar Products]
 UniProt Entry Name    KLOTB_HUMAN
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 UniProt Comments for KLB    Klotho beta: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding; fibroblast growth factor binding; fibroblast growth factor receptor binding; hydrolase activity, hydrolyzing O-glycosyl compounds

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of cell proliferation; carbohydrate metabolic process; insulin receptor signaling pathway; innate immune response
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 Research Articles on KLB    1. betaKlotho suppresses tumor growth in hepatocellular carcinoma by regulating Akt/GSK-3beta/cyclin D1 signaling pathway.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KLB recombinant proteinPathways associated with KLB recombinant protein
 Reference Product  PubMed Publications
 FGF21 recombinant protein  >23 publications with KLB and FGF21
 FGF19 recombinant protein  >18 publications with KLB and FGF19
 FGFR4 recombinant protein  >14 publications with KLB and FGFR4
 FGFR1 recombinant protein  >12 publications with KLB and FGFR1
 FGF23 recombinant protein  >5 publications with KLB and FGF23
 NR0B2 recombinant protein  >1 publications with KLB and NR0B2
 CYP8B1 recombinant protein  >1 publications with KLB and CYP8B1
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 FGFR Ligand Binding And Activation Pathway antibodies  FGFR Ligand Binding And Activation Pathway Diagram
Diseases associated with KLB recombinant proteinOrgans/Tissues associated with KLB recombinant protein
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >5 publications with KLB and Liver Diseases
 Fatty Liver Antibodies  >5 publications with KLB and Fatty Liver
 Neoplasms Antibodies  >4 publications with KLB and Neoplasms
 Disease Models, Animal Antibodies  >4 publications with KLB and Disease Models, Animal
 Weight Loss Antibodies  >2 publications with KLB and Weight Loss
 Carcinoma, Hepatocellular Antibodies  >2 publications with KLB and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >2 publications with KLB and Liver Neoplasms
 Inflammation Antibodies  >2 publications with KLB and Inflammation
 Cardiovascular Diseases Antibodies  >1 publications with KLB and Cardiovascular Diseases
 Necrosis Antibodies  >1 publications with KLB and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >24 publications with KLB and Liver
 Adipose Tissue Antibodies  >13 publications with KLB and Adipose Tissue
 Brain Antibodies  >2 publications with KLB and Brain
 Eye Antibodies  >1 publications with KLB and Eye
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