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mdeA recombinant protein :: L-Methionine gamma-Lyase Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS844459
Unit / Price
0.1 mg  /  $155 +1 FREE 8GB USB
0.5 mg  /  $345 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

L-Methionine gamma-Lyase (mdeA), Recombinant Protein

★Popular Item★
 Also Known As   

L-Methionine gamma-Lyase, Pseudomonas putida recombinant

 Product Synonym Names    L-Methionine-alpha-deamino-gamma-mercaptomethane-Lyase; Methioninase; METase; MGL; mdeA
 Product Gene Name   

mdeA recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P13254
 Host    E Coli
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 Purity/Purification    >=90%
 Form/Format    Lyophilized from 10 mg/ml in 20 mM potassium phosphate, 150 mM NaCl, pH 8.3.
Appearance: Lyophilized
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 Solubility/Reconstitution Instructions    Briefly spin down and reconstitute in water or phosphate buffer.
 Specific Activity    > or = 0.5 U/mg
 Unit Definition    One unit is defined as the amount of enzyme required to convert 1.0 mumole of L-methionine to alpha-ketobutyrate, methanethiol and ammonia per minute in 20 mM potassium phosphate, pH 8.3, 150 mM NaCl at 37 degree C
 Handling    Centrifuge the vial prior to opening.
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 Preparation and Storage    Store at 20 dergree C. Stable for at least 1 year as supplied. Avoid repeated freeze and thaw cycles.
 Other Notes    Small volumes of mdeA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for mdeA recombinant protein

   Background: Methionine gamma-lyase (EC 4.4.1.11) from Pseudomonas putida is a PLP-dependent enzyme which plays a central role in sulfur amino acid metabolism. METase catalyzes the alpha, gamma-elimination of methionine to alpha-ketobutyrate, methanethiol, and ammonia. METase also catalyzes the alpha, gamma-elimination of other sulfur containing amino acids, such as homocysteine, cysteine. Because of its ability to deplete methionine, METase has been considered as a viable component of cancer therapeutics against methionine-dependent tumor cells. METase has also been utilized to design drug targets for the infectious diseases caused by parasitic protozoa and anaerobic periodontal bacteria.
 Product Categories/Family for mdeA recombinant protein    Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)
 Applications Tested/Suitable for mdeA recombinant protein   

SDS-PAGE; HPLC

 Application Notes for mdeA recombinant protein    Recombinant bacterial Methionine Gamma-lyase can be used in inhibitor screening assays, activity studies, selectivity profiling, western blotting, ELISA, and numerous similar applications.
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 SDS-PAGE of mdeA recombinant protein    mdeA recombinant protein SDS-PAGE image
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 Test Data of mdeA recombinant protein    mdeA recombinant protein Test Data image
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NCBI/Uniprot data below describe general gene information for mdeA. It may not necessarily be applicable to this product.
 NCBI GI #    1708975
 NCBI Accession #    P13254.2 [Other Products]
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 UniProt Primary Accession #    P13254 [Other Products]
 Molecular Weight    47.1 kDa (1-398 aa, NT Poly-His Tag)
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 NCBI Official Full Name    Methionine gamma-lyase
 UniProt Protein Name    Methionine gamma-lyase
 UniProt Synonym Protein Names   
L-methioninase
 Protein Family    L-methionine gamma-lyase
 UniProt Gene Name    mdeA [Similar Products]
 UniProt Entry Name    MEGL_PSEPU
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 UniProt Comments for mdeA    Catalyzes the alpha,gamma-elimination of L-methionine to produce methanethiol, 2-oxobutanoate and ammonia (PubMed:8586629, PubMed:6742420). Is involved in L-methionine catabolism (PubMed:9190812). In fact, shows a multicatalytic function since it also catalyzes gamma-replacement of L-methionine with thiol compounds, alpha,gamma-elimination and gamma-replacement reactions of L-homocysteine and its S-substituted derivatives, O-substituted-L-homoserines and DL-selenomethionine, and, to a lesser extent, alpha,beta-elimination and beta-replacement reactions of L-cysteine, S-methyl-L-cysteine, and O-acetyl-L-serine (PubMed:6742420, PubMed:22785484). Also catalyzes deamination and gamma-addition reactions of L-vinylglycine (PubMed:6742420). Thus, the enzyme is able to cleave C-S, C-Se, and C-O bonds of sulfur, selenium, and oxygen amino acids, respectively (PubMed:6742420, PubMed:22785484).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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