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LATS2 recombinant protein

Scan QR to view Datasheet Catalog #    MBS515127
SDS-PAGE
Unit / Price
0.02 mg  /  $260 +1 FREE 8GB USB
0.05 mg  /  $415 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

LATS2, Recombinant Protein

★Popular Item★
 Also Known As   

LATS2, Unactive recombinant protein

 Product Synonym Names    KPM; FLJ13161
 Product Gene Name   

LATS2 recombinant protein

[Similar Products]
 Product Synonym Gene Name    KPM; FLJ13161 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    480-1088
 Chromosome Location    Chromosome: 13; NC_000013.10 (21547175..21635722, complement). Location: 13q11-q12
 OMIM    604861
 3D Structure    ModBase 3D Structure for Q9NRM7
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 Host    Sf9 insect cells
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 Purity/Purification    >80%
 Form/Format    Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
 Concentration    0.2ug/ul (lot specific)
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 Type    Recombinant Fusion Protein
 Species    Human
 Tag Information    GST tag
 Expression System    Sf9 insect cells using baculovirus
 Source Note    Recombinant human LATS2 (480-1088) was expressed by baculovirus in Sf9 insect cells
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 Preparation and Storage    Stability: 1yr At 70oC from date of shipment

Storage & Shipping:
Store product at 70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: ships on dry ice.
 Other Notes    Small volumes of LATS2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for LATS2 recombinant protein

   Recombinant human LATS2 (480-1088) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: LATS2 is a serine/threonine protein kinase belonging to the LATS tumor suppressor family (1). The kinase activity and two conserved domains within LATS2 are responsible for the suppression of tumorigenicity and inhibition of cell growth. LATS2 negatively regulates the cell cycle by controlling G1/S and/or G2/M transition. LATS2 interacts with the centrosomal proteins AURORA A and Ajuba and is required for accumulation of γ-tubulin and spindle formation at the onset of mitosis (2). LATS2 also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. LATS2 can also function as a co-repressor of androgen-responsive gene expression.
 Product Categories/Family for LATS2 recombinant protein    Unactive Kinase; Signaling Proteins - Unactive Kinases
 Applications Tested/Suitable for LATS2 recombinant protein   

Kinase Assay, Western Blot (WB)

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 SDS-PAGE of LATS2 recombinant protein    The purity was determined to be >80% by densitometry.
Approx. MW 95kDa
LATS2 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for LATS2. It may not necessarily be applicable to this product.
 NCBI GI #    126507090
 NCBI GeneID    26524
 NCBI Accession #    NM_014572 [Other Products]
 NCBI GenBank Nucleotide #    NM_014572 [Other Products]
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 UniProt Primary Accession #    Q9NRM7 [Other Products]
 UniProt Secondary Accession #    Q9P2X1; B1AM47 [Other Products]
 UniProt Related Accession #    Q9NRM7 [Other Products]
 Molecular Weight    ~98 kDa
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 NCBI Official Full Name    Homo sapiens large tumor suppressor kinase 2 (LATS2), mRNA
 NCBI Official Synonym Full Names    large tumor suppressor kinase 2
 NCBI Official Symbol    LATS2 [Similar Products]
 NCBI Official Synonym Symbols   
KPM
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase LATS2; warts-like kinase; serine/threonine kinase KPM; large tumor suppressor homolog 2; serine/threonine-protein kinase kpm; LATS, large tumor suppressor, homolog 2; kinase phosphorylated during mitosis protein; LATS (large tumor suppressor, Drosophila) homolog 2
 UniProt Protein Name    Serine/threonine-protein kinase LATS2
 UniProt Synonym Protein Names   
Kinase phosphorylated during mitosis protein; Large tumor suppressor homolog 2; Serine/threonine-protein kinase kpm; Warts-like kinase
 Protein Family    Serine/threonine-protein kinase
 UniProt Gene Name    LATS2 [Similar Products]
 UniProt Synonym Gene Names    KPM [Similar Products]
 UniProt Entry Name    LATS2_HUMAN
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 NCBI Summary for LATS2    This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008]
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 UniProt Comments for LATS2    Function: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ. Ref.1 Ref.6 Ref.8 Ref.11 Ref.15

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Subunit structure: Interacts with and is phosphorylated by AURKA. Binds to AR. Interacts with AJUBA during mitosis and this complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome. Interacts (via PPxY motif) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with SNAI1. Ref.7 Ref.8 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15

Subcellular location: Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cytoplasm. Cytoplasm › cytoskeleton › spindle pole. Nucleus. Note: Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment. Ref.6 Ref.7 Ref.15

Tissue specificity: Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined. Ref.1 Ref.5

Post-translational modification: Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3/MST2. Ref.1 Ref.7 Ref.9 Ref.15

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.Contains 1 AGC-kinase C-terminal domain.Contains 1 protein kinase domain.Contains 1 UBA domain.

Sequence caution: The sequence BAA92381.1 differs from that shown. Reason: Frameshift at positions 512 and 526.
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Product References and Citations for LATS2 recombinant protein

   1. Li, Y. et al: Lats2, a putative tumor suppressor, inhibits G1/S transition. Oncogene. 2003; 22(28):4398-405.
2. Yabuta, N. et al: Structure, expression, and chromosome mapping of LATS2, a mammalian homologue of the Drosophila tumor suppressor gene lats/warts. Genomics. 2000 63(2):263-70.
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 Research Articles on LATS2    1. Identifying novel kinases which modulate Estrogen Receptor alpha activity is relevant to therapeutics. LATS2 modulates Estrogen Receptor alpha-regulated gene transcription, through direct and/or indirect interactions with Estrogen Receptor alpha.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LATS2 recombinant proteinPathways associated with LATS2 recombinant protein
 Reference Product  PubMed Publications
 LATS1 recombinant protein  >46 publications with LATS2 and LATS1
 SAV1 recombinant protein  >9 publications with LATS2 and SAV1
 YAP1 recombinant protein  >8 publications with LATS2 and YAP1
 STK4 recombinant protein  >6 publications with LATS2 and STK4
 WWC1 recombinant protein  >3 publications with LATS2 and WWC1
 CASP3 recombinant protein  >2 publications with LATS2 and CASP3
 WWTR1 recombinant protein  >2 publications with LATS2 and WWTR1
 AMOTL1 recombinant protein  >1 publications with LATS2 and AMOTL1
 AMOTL2 recombinant protein  >1 publications with LATS2 and AMOTL2
 Products by Pathway  Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By Hippo Pathway antibodies  Signaling By Hippo Pathway Diagram
Diseases associated with LATS2 recombinant proteinOrgans/Tissues associated with LATS2 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >56 publications with LATS2 and Neoplasms
 Carcinoma Antibodies  >20 publications with LATS2 and Carcinoma
 Breast Neoplasms Antibodies  >11 publications with LATS2 and Breast Neoplasms
 Lung Neoplasms Antibodies  >10 publications with LATS2 and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >9 publications with LATS2 and Cell Transformation, Neoplastic
 Liver Neoplasms Antibodies  >5 publications with LATS2 and Liver Neoplasms
 Genital Diseases, Male Antibodies  >5 publications with LATS2 and Genital Diseases, Male
 Immune System Diseases Antibodies  >5 publications with LATS2 and Immune System Diseases
 Disease Models, Animal Antibodies  >4 publications with LATS2 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >4 publications with LATS2 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >14 publications with LATS2 and Lung
 Blood Antibodies  >7 publications with LATS2 and Blood
 Liver Antibodies  >6 publications with LATS2 and Liver
 Prostate Antibodies  >5 publications with LATS2 and Prostate
 Embryonic Tissue Antibodies  >5 publications with LATS2 and Embryonic Tissue
 Skin Antibodies  >4 publications with LATS2 and Skin
 Muscle Antibodies  >4 publications with LATS2 and Muscle
 Lymph Node Antibodies  >3 publications with LATS2 and Lymph Node
 Nerve Antibodies  >3 publications with LATS2 and Nerve
 Stomach Antibodies  >2 publications with LATS2 and Stomach
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