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LEPR recombinant protein :: Leptin Receptor (LEPR) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2010650 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
SDS-PAGE
Unit / Price
0.01 mg  /  $145 +1 FREE 8GB USB
0.05 mg  /  $275 +1 FREE 8GB USB
0.1 mg  /  $430 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Leptin Receptor (LEPR), Recombinant Protein

 Also Known As   

Recombinant Leptin Receptor (LEPR)

 Product Gene Name   

LEPR recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-G KRLDGPGIWS DWSTPRVFTT QDVIYFPPKI LTSVGSNVSF HCIYKKENKI VPSKEIVWWM NLAEKIPQSQ YDVVSDHVSK VTFFNLNETK PRGKFTYDAV YCCNEHECHH RYAELYVIDV NINISCETDG YLTKMTCRWS TSTIQSLAES TLQLRYHRSS LYCSDIPSIH PISEPKDCYL QSDGFYECIF QPIFLLSGYT MWIRINHSLG SLDSPPTCVL PDSVVKPL
 Chromosome Location    Chromosome: 1; NC_000001.10 (65886335..66103176). Location: 1p31
 OMIM    601007
 3D Structure    ModBase 3D Structure for P48357
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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 Organism    Homo sapiens (Human)
 Expression System    Prokaryotic expression
 Residues    Gly310~Leu538 (Accession # P48357) with two N-terminal Tags, His-tag and T7-tag
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of LEPR recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for LEPR recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for LEPR recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of LEPR recombinant protein    LEPR recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for LEPR. It may not necessarily be applicable to this product.
 NCBI GI #    51093379
 NCBI GeneID    3953
 NCBI Accession #    NP_001003679.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001003679.3 [Other Products]
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 UniProt Primary Accession #    P48357 [Other Products]
 UniProt Secondary Accession #    Q13592; Q13593; Q13594; Q92919; Q92920; Q92921 [Other Products]
 UniProt Related Accession #    P48357 [Other Products]
 Molecular Weight    29.9kDa
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 NCBI Official Full Name    leptin receptor isoform 3
 NCBI Official Synonym Full Names    leptin receptor
 NCBI Official Symbol    LEPR [Similar Products]
 NCBI Official Synonym Symbols   
OBR; OB-R; CD295; LEP-R; LEPRD
[Similar Products]
 NCBI Protein Information    leptin receptor; huB219; OB receptor
 UniProt Protein Name    Leptin receptor
 UniProt Synonym Protein Names   
HuB219; OB receptor
 Protein Family    Leptin receptor
 UniProt Gene Name    LEPR [Similar Products]
 UniProt Synonym Gene Names    DB; OBR; LEP-R; OB-R [Similar Products]
 UniProt Entry Name    LEPR_HUMAN
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 NCBI Summary for LEPR    The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]
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 UniProt Comments for LEPR    LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: extracellular region; plasma membrane; integral to membrane; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity

Biological Process: cholesterol metabolic process; cell surface receptor linked signal transduction; negative regulation of hydrolase activity; negative regulation of gluconeogenesis; cytokine and chemokine mediated signaling pathway; multicellular organismal development; energy reserve metabolic process

Disease: Leptin Receptor Deficiency
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 Research Articles on LEPR    1. the Arg109Arg genotype of LEPR seems to be protective from cardiovascular events and death and this phenomenon seems to be independent of the traditional risk factors for atherosclerosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LEPR recombinant proteinPathways associated with LEPR recombinant protein
 Reference Product  PubMed Publications
 LEP recombinant protein  >155 publications with LEPR and LEP
 STAT3 recombinant protein  >43 publications with LEPR and STAT3
 JAK2 recombinant protein  >12 publications with LEPR and JAK2
 INS recombinant protein  >7 publications with LEPR and INS
 IRS1 recombinant protein  >6 publications with LEPR and IRS1
 SOCS3 recombinant protein  >6 publications with LEPR and SOCS3
 APOD recombinant protein  >2 publications with LEPR and APOD
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Leptin Signaling Pathway antibodies  Leptin Signaling Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
Diseases associated with LEPR recombinant proteinOrgans/Tissues associated with LEPR recombinant protein
 Disease Name  Pubmed Publications
 Peripheral Nervous System Diseases Antibodies  >398 publications with LEPR and Peripheral Nervous System Diseases
 Drug-Related Side Effects and Adverse Reactions Antibodies  >349 publications with LEPR and Drug-Related Side Effects and Adverse Reactions
 Obesity Antibodies  >331 publications with LEPR and Obesity
 Overweight Antibodies  >224 publications with LEPR and Overweight
 Diabetes Mellitus Antibodies  >193 publications with LEPR and Diabetes Mellitus
 Inflammation Antibodies  >189 publications with LEPR and Inflammation
 Neoplasms Antibodies  >154 publications with LEPR and Neoplasms
 Necrosis Antibodies  >106 publications with LEPR and Necrosis
 Diabetes Mellitus, Type 2 Antibodies  >105 publications with LEPR and Diabetes Mellitus, Type 2
 Cardiovascular Diseases Antibodies  >103 publications with LEPR and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >1384 publications with LEPR and Skin
 Nerve Antibodies  >673 publications with LEPR and Nerve
 Muscle Antibodies  >225 publications with LEPR and Muscle
 Liver Antibodies  >219 publications with LEPR and Liver
 Eye Antibodies  >179 publications with LEPR and Eye
 Bone Antibodies  >153 publications with LEPR and Bone
 Connective Tissue Antibodies  >126 publications with LEPR and Connective Tissue
 Vascular Antibodies  >121 publications with LEPR and Vascular
 Brain Antibodies  >112 publications with LEPR and Brain
 Adipose Tissue Antibodies  >110 publications with LEPR and Adipose Tissue
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