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Lep recombinant protein :: Leptin Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS142104 Lep recombinant protein
Unit / Price
0.2 mg  /  $140 +1 FREE 8GB USB
1 mg  /  $205 +1 FREE 8GB USB
5 mg  /  $505 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Leptin, Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Rat Leptin

 Product Synonym Names    Leptin Rat; Leptin Rat Recombinant; OB Protein; Obesity Protein; OBS; Obesity factor; rLeptin
 Product Gene Name   

Lep recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pr o-Ile-His
 3D Structure    ModBase 3D Structure for P50596
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 Host    E Coli
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 Purity/Purification    Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
 Form/Format    The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Sterile Filtered White lyophilized (freeze-dried) powder.
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 Solubility    It is recommended to reconstitute the lyophilized Leptin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
 Protein Content    Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of Leptin rat as a Reference Standard.
 Biological Activity    Biological activity of Leptin Rat is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
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 Preparation and Storage    Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Leptin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
 Other Notes    Small volumes of Lep recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Lep recombinant protein

   Description: Leptin Rat Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16 kDa.The Leptin is purified by proprietary chromatographic techniques.

Introduction: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
 Product Categories/Family for Lep recombinant protein    CYTOKINES AND GROWTH FACTORS; Growth Factors; Leptin
NCBI/Uniprot data below describe general gene information for Lep. It may not necessarily be applicable to this product.
 NCBI GI #    6981148
 NCBI GeneID    25608
 NCBI Accession #    NP_037208.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_013076.3 [Other Products]
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 UniProt Primary Accession #    P50596 [Other Products]
 UniProt Related Accession #    P50596 [Other Products]
 Molecular Weight    18,866 Da [Similar Products]
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 NCBI Official Full Name    leptin
 NCBI Official Synonym Full Names    leptin
 NCBI Official Symbol    Lep [Similar Products]
 NCBI Official Synonym Symbols   
OB; obese
[Similar Products]
 NCBI Protein Information    leptin; obesity factor
 UniProt Protein Name    Leptin
 UniProt Synonym Protein Names   
Obesity factor
 Protein Family    Leptin
 UniProt Gene Name    Lep [Similar Products]
 UniProt Synonym Gene Names    Ob [Similar Products]
 UniProt Entry Name    LEP_RAT
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 NCBI Summary for Lep    involved in regulation of body weight, and may have a role in angiogenesis [RGD, Feb 2006]
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 UniProt Comments for Lep    Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways () (PubMed:11677594). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity () (PubMed:11460888, PubMed:10482243, PubMed:9003024, PubMed:11677594). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides () (PubMed:10482243, PubMed:9003024). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption (). Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (). May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression (). Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) (PubMed:11460888). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells (Probable). Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (). Increases CD4+CD25- T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation ().
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 Research Articles on Lep    1. The metabolic response to postnatal leptin varies with age.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Lep recombinant proteinPathways associated with Lep recombinant protein
 Reference Product  PubMed Publications
 Pparg recombinant protein  >37 publications with Lep and Pparg
 Tnf recombinant protein  >28 publications with Lep and Tnf
 Npy recombinant protein  >22 publications with Lep and Npy
 Stat3 recombinant protein  >16 publications with Lep and Stat3
 Ghrl recombinant protein  >11 publications with Lep and Ghrl
 Agrp recombinant protein  >10 publications with Lep and Agrp
 Retn recombinant protein  >9 publications with Lep and Retn
 Leprot recombinant protein  >3 publications with Lep and Leprot
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Incretin Synthesis, Secretion, And Inactivation Pathway antibodies  Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
Diseases associated with Lep recombinant proteinOrgans/Tissues associated with Lep recombinant protein
 Disease Name  Pubmed Publications
 Obesity Antibodies  >355 publications with Lep and Obesity
 Pain Antibodies  >174 publications with Lep and Pain
 Insulin Resistance Antibodies  >114 publications with Lep and Insulin Resistance
 Hyperinsulinism Antibodies  >76 publications with Lep and Hyperinsulinism
 Weight Gain Antibodies  >60 publications with Lep and Weight Gain
 Weight Loss Antibodies  >58 publications with Lep and Weight Loss
 Cardiovascular Diseases Antibodies  >52 publications with Lep and Cardiovascular Diseases
 Inflammation Antibodies  >44 publications with Lep and Inflammation
 Fatty Liver Antibodies  >44 publications with Lep and Fatty Liver
 Liver Diseases Antibodies  >39 publications with Lep and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >219 publications with Lep and Brain
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