Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Recombinant Protein rightarrow Lep  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • C5AR2 - GPR77 - C5L2 Antibody
 • Chalcone synthase (CHS) Recombinant Protein
 • Cathelicidin Antimicrobial Peptide (CAMP) Recombinant Protein
 • Cathepsin K (CTSK) ELISA Kit
 • Parathyroid Hormone (PTH) ELISA Kit
 • Epidermal Growth Factor Receptor 2 Extracellular Domain (HER... ELISA Kit
 • Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Antibody
 • 1.5M Tris-HCl, pH8.8 Buffer
 • Trichomonas vaginalis Antibody
 • Amine Oxidase, Copper Containing 3 (AOC3) ELISA Kit
 • DCXR Antibody
 • West Nile Virus, Envelope Antibody
 • HNRPA2B1 ELISA Kit
 • Soluble Cluster of Differentiation 86 (SCD86) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

Lep recombinant protein :: Leptin Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS203097
SDS-Page
Unit / Price
0.1 mg  /  $375 +1 FREE 8GB USB
0.5 mg  /  $925 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Leptin, Recombinant Protein

 Also Known As   

Leptin, 22-167 aa, Mouse, E Coli

 Product Synonym Names    LEP; OB; OBS; Leptin Mouse; Obesity factor; Obese protein; Leptin
 Product Gene Name   

Lep recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Sequence    MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC
 3D Structure    ModBase 3D Structure for P41160
Section Bar
 Host    E Coli
Section Bar
 Purity/Purification    > 95% by SDS-PAGE
 Form/Format    Liquid. In phosphate buffered saline pH7.4
 Concentration    1 mg/ml (determined by Bradford assay) (lot specific)
Section Bar
 Antigen Species    Mouse
Section Bar
 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of Lep recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for Lep recombinant protein

   Leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. Originally discovered in the mouse, the cDNA for leptin has been so far cloned from a variety of species including human, rat, cow, chicken, pig, sheep and dog. Recombinant mouse leptin was overexpressed as insoluble protein aggregate in E Coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer
 Product Categories/Family for Lep recombinant protein    Hormones & Cytokines
 Applications Tested/Suitable for Lep recombinant protein   

SDS-PAGE

Section Bar
 SDS-Page of Lep recombinant protein    Lep recombinant protein SDS-Page image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for Lep. It may not necessarily be applicable to this product.
 NCBI GI #    6678678
 NCBI GeneID    16846
 NCBI Accession #    NP_032519 [Other Products]
 NCBI GenBank Nucleotide #    NM_008493.3 [Other Products]
Section Bar
 UniProt Primary Accession #    P41160 [Other Products]
 UniProt Related Accession #    P41160 [Other Products]
 Molecular Weight    16 kDa (147 aa), confirmed by MALDI-TOF. [Similar Products]
Table BarTOPTable Bar
 NCBI Official Full Name    leptin
 NCBI Official Synonym Full Names    leptin
 NCBI Official Symbol    Lep [Similar Products]
 NCBI Official Synonym Symbols   
ob; obese
[Similar Products]
 NCBI Protein Information    leptin
 UniProt Protein Name    Leptin
 UniProt Synonym Protein Names   
Obesity factor
 Protein Family    Leptin
 UniProt Gene Name    Lep [Similar Products]
 UniProt Synonym Gene Names    Ob [Similar Products]
Section Bar
 UniProt Comments for Lep    Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (PubMed:15899045, PubMed:16825198, PubMed:11373681, PubMed:12594516, PubMed:20620997). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity () (PubMed:8589726, PubMed:10660043, PubMed:25383904, PubMed:25060689, PubMed:9732873, PubMed:12594516). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides () (PubMed:20620997, PubMed:11373681). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption. Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways () (PubMed:16825198, PubMed:20620997). May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression (). Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) (PubMed:16825198). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells (Probable). Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (PubMed:15899045). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (). Increases CD4+CD25- T cells proliferation and reduces autophagy during TCR (T cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation (PubMed:25060689).
Table BarTOPTable Bar
 

Product References and Citations for Lep recombinant protein

   Jeong KJ., et al. (1999) Appl Environ Microbiol. 65 (7), 3027-32.; Anini Y., et al. (2003) Diabetes. 52 (2), 252-259.; Hyogo H., et al. (2002) J. Biol. Chem. 277 (37), 34117-34124.;
Section Bar
 Research Articles on Lep    1. Thus, Mc4r in the PVH appears to be required for early-life programming of hypertension arising from either maternal obesity or neonatal hyperleptinemia. Early-life exposure of the PVH to maternal obesity through postnatal elevation of leptin may have long-term consequences for cardiovascular health.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with Lep recombinant proteinPathways associated with Lep recombinant protein
 Reference Product  PubMed Publications
 Lepr recombinant protein  >259 publications with Lep and Lepr
 Adipoq recombinant protein  >64 publications with Lep and Adipoq
 Stat3 recombinant protein  >26 publications with Lep and Stat3
 Ghrl recombinant protein  >15 publications with Lep and Ghrl
 Jak2 recombinant protein  >8 publications with Lep and Jak2
 Irs1 recombinant protein  >7 publications with Lep and Irs1
 Socs3 recombinant protein  >3 publications with Lep and Socs3
 Irs2 recombinant protein  >1 publications with Lep and Irs2
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Genes Pathway antibodies  Adipogenesis Genes Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Incretin Synthesis, Secretion, And Inactivation Pathway antibodies  Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
Diseases associated with Lep recombinant proteinOrgans/Tissues associated with Lep recombinant protein
 Disease Name  Pubmed Publications
 LEPTIN DEFICIENCY OR DYSFUNCTION Antibodies  >1865655 publications with Lep and LEPTIN DEFICIENCY OR DYSFUNCTION
 Obesity Antibodies  >479 publications with Lep and Obesity
 Insulin Resistance Antibodies  >152 publications with Lep and Insulin Resistance
 Hyperinsulinism Antibodies  >98 publications with Lep and Hyperinsulinism
 Weight Gain Antibodies  >81 publications with Lep and Weight Gain
 Inflammation Antibodies  >80 publications with Lep and Inflammation
 Weight Loss Antibodies  >76 publications with Lep and Weight Loss
 Metabolic Syndrome Antibodies  >65 publications with Lep and Metabolic Syndrome
 Diabetes Mellitus, Type 2 Antibodies  >56 publications with Lep and Diabetes Mellitus, Type 2
 Fatty Liver Antibodies  >56 publications with Lep and Fatty Liver
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >268 publications with Lep and Brain
 Adipose Tissue Antibodies  >222 publications with Lep and Adipose Tissue
 Skin Antibodies  >131 publications with Lep and Skin
 Bone Marrow Antibodies  >16 publications with Lep and Bone Marrow
 Bladder Antibodies  >7 publications with Lep and Bladder
 Salivary Gland Antibodies  >5 publications with Lep and Salivary Gland
 Thymus Antibodies  >4 publications with Lep and Thymus
 Mammary Gland Antibodies  >4 publications with Lep and Mammary Gland
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions