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Lep recombinant protein :: Leptin Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS203097
SDS-PAGE
Unit / Price
0.1 mg  /  $375 +1 FREE 8GB USB
0.5 mg  /  $925 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Leptin, Recombinant Protein

 Also Known As   

Leptin Mouse, Recombinant, E Coli

 Product Synonym Names    LEP; OB; OBS; Leptin Mouse; Obesity factor; Obese protein; Leptin
 Product Gene Name   

Lep recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC
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 Purity/Purification    > 95% by SDS - PAGE
 Form/Format    Liquid. In Phosphate buffered saline pH7.4
 Concentration    1 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Mouse
 Expression System    E Coli
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of Lep recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for Lep recombinant protein

   Leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. Originally discovered in the mouse, the cDNA for leptin has been so far cloned from a variety of species including human, rat, cow, chicken, pig, sheep and dog. Recombinant mouse leptin was overexpressed as insoluble protein aggregate in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer
 Product Categories/Family for Lep recombinant protein    Hormones & Cytokines
 Applications Tested/Suitable for Lep recombinant protein   

SDS-PAGE

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 SDS-PAGE of Lep recombinant protein    Lep recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for Lep. It may not necessarily be applicable to this product.
 NCBI GI #    6678678
 NCBI GeneID    16846
 NCBI Accession #    NP_032519 [Other Products]
 NCBI GenBank Nucleotide #    NM_008493.3 [Other Products]
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 UniProt Related Accession #    P41160 [Other Products]
 Molecular Weight    16 kDa (147 aa), confirmed by MALDI-TOF. [Similar Products]
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 NCBI Official Full Name    leptin
 NCBI Official Synonym Full Names    leptin
 NCBI Official Symbol    Lep [Similar Products]
 NCBI Official Synonym Symbols   
ob; obese
[Similar Products]
 NCBI Protein Information    leptin; obesity factor
 UniProt Protein Name    Leptin
 UniProt Synonym Protein Names   
Obesity factor
 Protein Family    Leptin
 UniProt Gene Name    Lep [Similar Products]
 UniProt Synonym Gene Names    Ob [Similar Products]
 UniProt Entry Name    LEP_MOUSE
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 UniProt Comments for Lep    leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Hormone; Secreted; Cell development/differentiation; Secreted, signal peptide

Cellular Component: extracellular space; cell; cytoplasm; extracellular region; intracellular

Molecular Function: protein binding; peptide hormone receptor binding; growth factor activity; hormone activity

Biological Process: response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; positive regulation of JAK-STAT cascade; regulation of steroid biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; glucose homeostasis; negative regulation of metabolic process; negative regulation of appetite; positive regulation of tyrosine phosphorylation of Stat3 protein; response to insulin stimulus; positive regulation of MAPKKK cascade; regulation of cholesterol absorption; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; regulation of metabolic process; placenta development; central nervous system neuron development; cholesterol metabolic process; positive regulation of developmental growth; regulation of protein amino acid phosphorylation; eating behavior; bile acid metabolic process; glucose metabolic process; adult feeding behavior; fatty acid catabolic process; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; positive regulation of protein import into nucleus; glycerol biosynthetic process; insulin secretion; energy reserve metabolic process; hormone metabolic process; regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway; lipid metabolic process; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis
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Product References and Citations for Lep recombinant protein

   Jeong KJ., et al. (1999) Appl Environ Microbiol. 65(7), 3027-32.
Anini Y., et al. (2003) Diabetes. 52(2), 252-259.
Hyogo H., et al. (2002) J. Biol. Chem. 277(37), 34117-34124.
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 Research Articles on Lep    1. Elevated leptin levels alone do not induce an inflammatory response in the placenta.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Lep recombinant proteinPathways associated with Lep recombinant protein
 Reference Product  PubMed Publications
 Lepr recombinant protein  >174 publications with Lep and Lepr
 Pparg recombinant protein  >37 publications with Lep and Pparg
 Tnf recombinant protein  >28 publications with Lep and Tnf
 Npy recombinant protein  >22 publications with Lep and Npy
 Stat3 recombinant protein  >16 publications with Lep and Stat3
 Ghrl recombinant protein  >11 publications with Lep and Ghrl
 Retn recombinant protein  >9 publications with Lep and Retn
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Incretin Synthesis, Secretion, And Inactivation Pathway antibodies  Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
Diseases associated with Lep recombinant proteinOrgans/Tissues associated with Lep recombinant protein
 Disease Name  Pubmed Publications
 Obesity Antibodies  >354 publications with Lep and Obesity
 Pain Antibodies  >173 publications with Lep and Pain
 Insulin Resistance Antibodies  >113 publications with Lep and Insulin Resistance
 Hyperinsulinism Antibodies  >76 publications with Lep and Hyperinsulinism
 Weight Gain Antibodies  >60 publications with Lep and Weight Gain
 Weight Loss Antibodies  >58 publications with Lep and Weight Loss
 Cardiovascular Diseases Antibodies  >52 publications with Lep and Cardiovascular Diseases
 Inflammation Antibodies  >44 publications with Lep and Inflammation
 Fatty Liver Antibodies  >44 publications with Lep and Fatty Liver
 Liver Diseases Antibodies  >39 publications with Lep and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >216 publications with Lep and Brain
 Adipose Tissue Antibodies  >166 publications with Lep and Adipose Tissue
 Skin Antibodies  >108 publications with Lep and Skin
 Bone Marrow Antibodies  >9 publications with Lep and Bone Marrow
 Salivary Gland Antibodies  >5 publications with Lep and Salivary Gland
 Bladder Antibodies  >5 publications with Lep and Bladder
 Mammary Gland Antibodies  >4 publications with Lep and Mammary Gland
 Thymus Antibodies  >3 publications with Lep and Thymus
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