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| Product Name
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Lysyl Oxidase Like Protein 1 (LOXL1), Recombinant Protein
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| Also Known As
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Recombinant Lysyl Oxidase Like Protein 1 (LOXL1)
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Sequence
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The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-LPDLVP D PNYVQASTYV QRAHLYSLRC AAEEKCLAST AYAPEATDYD VRVLLRFPQR VKNQGTADFL PNRPRHTWEW HSCHQHYHSM DEFSHYDLLD AATGKKVAEG HKASFCLEDS TCDFGNLKRY ACTSHTQGLS PGCYDTYNAD IDCQWIDITD VQPGNYILKV HVNPKYIVLE SDFTNNVVRC NIHYTGRYVS ATNCKIVQS
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| Chromosome Location
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Chromosome: 15; NC_000015.9 (74218789..74244478). Location: 15q22
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| OMIM
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153456
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| 3D Structure
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ModBase 3D Structure for Q08397
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| Host
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E Coli
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| Purity/Purification
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> 95%
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| Form/Format
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Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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| Organism
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Homo sapiens (Human)
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| Expression System
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Prokaryotic expression
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| Residues
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Leu369~Ser574 (Accession # Q08397) with N-terminal His-Tag
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| Endotoxin Level
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<1.0EU per 1ug (determined by the LAL method)
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| Reconstitution
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Reconstitute in sterile PBS, pH7.2-pH7.4.
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| Preparation and Storage
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Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
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| Other Notes
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Small volumes of LOXL1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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| Applications Tested/Suitable for LOXL1 recombinant protein
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SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
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TOP
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| NCBI/Uniprot data below describe general gene information for LOXL1. It may not necessarily be applicable to this product. |
| NCBI GI #
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67782346
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| NCBI GeneID
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4016
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| NCBI Accession #
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NP_005567.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_005576.2
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[Other Products]
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| UniProt Primary Accession #
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Q08397
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[Other Products]
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| UniProt Secondary Accession #
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Q6NUL3; Q96BW7
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[Other Products]
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| UniProt Related Accession #
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Q08397
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[Other Products]
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| Molecular Weight
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25.0kDa
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TOP
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| NCBI Official Full Name
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lysyl oxidase homolog 1 preproprotein
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| NCBI Official Synonym Full Names
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lysyl oxidase-like 1
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| NCBI Official Symbol
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LOXL1
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[Similar Products]
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| NCBI Official Synonym Symbols
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LOL; LOXL
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[Similar Products]
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| NCBI Protein Information
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lysyl oxidase homolog 1; lysyl oxidase-like protein 1
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| UniProt Protein Name
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Lysyl oxidase homolog 1
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| UniProt Synonym Protein Names
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Lysyl oxidase-like protein 1
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| Protein Family
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Lysyl oxidase
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| UniProt Gene Name
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LOXL1
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[Similar Products]
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| UniProt Synonym Gene Names
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LOXL; LOL
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[Similar Products]
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| UniProt Entry Name
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LOXL1_HUMAN
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| NCBI Summary for LOXL1
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This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]
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| UniProt Comments for LOXL1
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LOXL1: Active on elastin and collagen substrates. Genetic variations in LOXL1 are a cause of susceptibility to exfoliation syndrome (XFS); also called exfoliation glaucoma (XFG). XFS is a disorder characterized by accumulation of abnormal fibrillar deposits in the anterior segment of the eye. In addition to being a cause of glaucoma and glaucomatous optic neuropathy, exfoliation syndrome has also been associated with lens zonule weakness, cataract formation, and systemic vascular complications due to deposition of exfoliation material in extraocular tissues. Susceptibility to exfoliation syndrome is conferred by a risk haplotype that includes two LOXL1 coding non-synonymous SNPs (Arg141Leu and Gly153Asp) and one intronic SNP. Arg141Leu and Gly153Asp are sufficient to confer disease susceptibility in some populations. Belongs to the lysyl oxidase family.
Protein type: Oxidoreductase; Secreted; EC 1.4.3.-; Secreted, signal peptide; Extracellular matrix
Chromosomal Location of Human Ortholog: 15q22
Cellular Component: extracellular matrix; extracellular space; extracellular region; acrosome; basement membrane
Molecular Function: copper ion binding; oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
Biological Process: extracellular matrix organization and biogenesis; response to lipopolysaccharide; protein amino acid deamination
Disease: Exfoliation Syndrome
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TOP
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| Research Articles on LOXL1
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1. LOXL1 is more abundant in the deposits in the iris region and, alternatively APOE is concentrated in the PEX material accumulated in the pupillary area of the anterior lens capsule.
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with LOXL1 recombinant protein | Pathways associated with LOXL1 recombinant protein |
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| Diseases associated with LOXL1 recombinant protein | Organs/Tissues associated with LOXL1 recombinant protein |
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TOP
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Recombinant Protein 
