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MBD3 recombinant protein

Scan QR to view Datasheet Catalog #    MBS204158
SDS-Page
Unit / Price
0.05 mg  /  $375 +1 FREE 8GB USB
0.25 mg  /  $925 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MBD3, Recombinant Protein

★Popular Item★
 Also Known As   

MBD3, 1-291aa, Human, His tag, E Coli

 Product Synonym Names    Methyl-CpG-binding domain protein 3; Methyl CpG binding domain protein 3; Methyl-CpG-binding protein MBD3
 Product Gene Name   

MBD3 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MGSMERKRWE CPALPQGWER EEVPRRSGLS AGHRDVFYYS PSGKKFRSKP QLARYLGGSM DLSTFDFRTG KMLMSKMNKS RQRVRYDSSN QVKGKPDLNT ALPVRQTASI FKQPVTKITN HPSNKVKSDP QKAVDQPRQL FWEKKLSGLN AFDIAEELVK TMDLPKGLQG VGPGCTDETL LSAIASALHT STMPITGQLS AAVEKNPGVW LNTTQPLCKA FMVTDEDIRK QEELVQQVRK RLEEALMADM LAHVEELARD GEAPLDKACA EDDDEEDEEE EEEEPDPDPE MEHV
 OMIM    AF072247 mRNA
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 Host    E Coli
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 Purity/Purification    > 85% by SDS-PAGE
 Form/Format    Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT, 1mM EDTA
 Concentration    0.25 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag Information    His-tag
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of MBD3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for MBD3 recombinant protein

   Methyl-CpG-binding domain protein 3, also known as MBD3, is a member of the MBD family of transcriptional repressors. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Recombinant human MBD3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
 Product Categories/Family for MBD3 recombinant protein    Epigenomics
 Applications Tested/Suitable for MBD3 recombinant protein   

SDS-PAGE

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 SDS-Page of MBD3 recombinant protein    MBD3 recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for MBD3. It may not necessarily be applicable to this product.
 NCBI GI #    4505119
 NCBI GeneID    53615
 NCBI Accession #    NP_003917 [Other Products]
 NCBI GenBank Nucleotide #    NM_003926.5 [Other Products]
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 UniProt Secondary Accession #    Q6PIL9; Q6PJZ9; Q86XF4; A8K4B7; D6W5Z2 [Other Products]
 UniProt Related Accession #    O95983 [Other Products]
 Molecular Weight    35.2 kDa (314aa) confirmed by MALDI-TOF
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 NCBI Official Synonym Full Names    methyl-CpG binding domain protein 3
 NCBI Official Symbol    MBD3 [Similar Products]
 NCBI Protein Information    methyl-CpG-binding domain protein 3
 UniProt Protein Name    Methyl-CpG-binding domain protein 3
 UniProt Synonym Protein Names   
Methyl-CpG-binding protein MBD3
 Protein Family    Methyl-CpG-binding domain protein
 UniProt Gene Name    MBD3 [Similar Products]
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 NCBI Summary for MBD3    DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
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 UniProt Comments for MBD3    Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.
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Product References and Citations for MBD3 recombinant protein

   Nan X., et al. (1998) Nature. 393:386-389; Hendrich B., et al. (1999) Mamm Genome. 10: 906-912.
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 Research Articles on MBD3    1. MBD3 inhibits epithelial-mesenchymal transition in pancreatic cancer cells probably via TGF-beta/Smad signaling and may be a candidate for diagnostics and prognosis of pancreatic cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MBD3 recombinant proteinPathways associated with MBD3 recombinant protein
 Reference Product  PubMed Publications
 HDAC1 recombinant protein  >21 publications with MBD3 and HDAC1
 CHD4 recombinant protein  >12 publications with MBD3 and CHD4
 HDAC2 recombinant protein  >11 publications with MBD3 and HDAC2
 MTA1 recombinant protein  >8 publications with MBD3 and MTA1
 MTA2 recombinant protein  >8 publications with MBD3 and MTA2
 MTA3 recombinant protein  >4 publications with MBD3 and MTA3
 GATAD2B recombinant protein  >3 publications with MBD3 and GATAD2B
 RBBP7 recombinant protein  >3 publications with MBD3 and RBBP7
 GATAD2A recombinant protein  >2 publications with MBD3 and GATAD2A
 RBBP4 recombinant protein  >2 publications with MBD3 and RBBP4
 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 Cytosine Methylation Pathway antibodies  Cytosine Methylation Pathway Diagram
 ERCC6 (CSB) And EHMT2 (G9a) Positively Regulate RRNA Expression Pathway antibodies  ERCC6 (CSB) And EHMT2 (G9a) Positively Regulate RRNA Expression Pathway Diagram
 Epigenetic Regulation Of Gene Expression Pathway antibodies  Epigenetic Regulation Of Gene Expression Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 HDACs Deacetylate Histones Pathway antibodies  HDACs Deacetylate Histones Pathway Diagram
 Positive Epigenetic Regulation Of RRNA Expression Pathway antibodies  Positive Epigenetic Regulation Of RRNA Expression Pathway Diagram
 RNA Polymerase I Promoter Clearance Pathway antibodies  RNA Polymerase I Promoter Clearance Pathway Diagram
Diseases associated with MBD3 recombinant proteinOrgans/Tissues associated with MBD3 recombinant protein
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >11 publications with MBD3 and Disease Models, Animal
 Nervous System Diseases Antibodies  >9 publications with MBD3 and Nervous System Diseases
 Lung Neoplasms Antibodies  >5 publications with MBD3 and Lung Neoplasms
 Adenocarcinoma Antibodies  >4 publications with MBD3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >4 publications with MBD3 and Breast Neoplasms
 Inflammation Antibodies  >3 publications with MBD3 and Inflammation
 Liver Diseases Antibodies  >3 publications with MBD3 and Liver Diseases
 Learning Disorders Antibodies  >2 publications with MBD3 and Learning Disorders
 Liver Neoplasms Antibodies  >2 publications with MBD3 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >2 publications with MBD3 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >20 publications with MBD3 and Brain
 Blood Antibodies  >14 publications with MBD3 and Blood
 Skin Antibodies  >12 publications with MBD3 and Skin
 Liver Antibodies  >10 publications with MBD3 and Liver
 Lung Antibodies  >9 publications with MBD3 and Lung
 Embryonic Tissue Antibodies  >8 publications with MBD3 and Embryonic Tissue
 Nerve Antibodies  >6 publications with MBD3 and Nerve
 Eye Antibodies  >6 publications with MBD3 and Eye
 Testis Antibodies  >4 publications with MBD3 and Testis
 Connective Tissue Antibodies  >3 publications with MBD3 and Connective Tissue
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