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MMP3 recombinant protein :: Matrix Metalloproteinase 3 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS637729 MMP3 recombinant protein
Unit / Price
0.002 mg  /  $345 +1 FREE 8GB USB
0.01 mg  /  $420 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Matrix Metalloproteinase 3 (MMP3), Recombinant Protein

★Popular Item★
 Also Known As   

Matrix Metalloproteinase 3, Recombinant, Human (MMP-3, Stromelysin-1, SL-1, Transin)

 Product Gene Name   

MMP3 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 11; NC_000011.9 (102706528..102714342, complement). Location: 11q22.3
 OMIM    185250
 3D Structure    ModBase 3D Structure for P08254
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 Host    HEK293
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 Purity/Purification    >95% (SDS-PAGE)
 Form/Format    Supplied as a liquid in in Tris, CaCl2, sodium chloride and Brij35.
 Concentration    0.3mg/ml (lot specific)
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 Biological Activity    The activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 150 pmoles/min/ug.
 Actication Protocol    1.Dilute MMP3 to 20ug/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM sodium chloride, 0.05% and Brij35, pH 7.5
2. Activate MMP3 by adding Chymotrypsin to final concentration of 5ug/ml.
3. Incubate at 37 degree C for 30 minutes.
4.Stop activation with 2mM PMSF. Pre-warm the PMSF to 37 degree C prior to adding to sample.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquot are stable for 6 months after receipt at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of MMP3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for MMP3 recombinant protein

   Matrix metalloproteinases are important regulators of extracellular matrix molecules and cell-cell signaling and are involved in the regulation of the pericellular space, including the cleavage of matrix proteins. In particular, MMP3 has been shown to be effective in cleaving heparan sulfate proteoglycans. Purified recombinant MMP3 can directly cleave agrin, and it can also remove agrin from synaptic basal lamina. MMP3 activity is itself regulated as activation of MMP3 is lost in denervated muscles.

Recombinant Human MMP-3 produced in HEK293 cells is a proform of the Human MMP3 [Tyr18-Cys477 (Lys45Glu)] and fused with a his-tag at the C-terminus. Matrix Metalloproteinase-3 is purified by proprietary chromatographic techniques.
 Product Categories/Family for MMP3 recombinant protein    Molecular Biology; MB-Matrix Metalloproteinases
NCBI/Uniprot data below describe general gene information for MMP3. It may not necessarily be applicable to this product.
 NCBI GI #    90287774
 NCBI GeneID    4314
 NCBI Accession #    ABD92797.1 [Other Products]
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 UniProt Primary Accession #    P08254 [Other Products]
 UniProt Secondary Accession #    Q3B7S0; Q6GRF8; B2R8B8 [Other Products]
 UniProt Related Accession #    P08254 [Other Products]
 Molecular Weight    52kD [Similar Products]
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 NCBI Official Full Name    matrix metalloproteinase 3
 NCBI Official Synonym Full Names    matrix metallopeptidase 3 (stromelysin 1, progelatinase)
 NCBI Official Symbol    MMP3 [Similar Products]
 NCBI Official Synonym Symbols   
SL-1; STMY; STR1; CHDS6; MMP-3; STMY1
[Similar Products]
 NCBI Protein Information    stromelysin-1; transin-1; proteoglycanase; matrix metalloproteinase-3; matrix metalloproteinase 3 (stromelysin 1, progelatinase)
 UniProt Protein Name    Stromelysin-1
 UniProt Synonym Protein Names   
Matrix metalloproteinase-3; MMP-3; Transin-1
 Protein Family    Stromelysin
 UniProt Gene Name    MMP3 [Similar Products]
 UniProt Synonym Gene Names    STMY1; SL-1; MMP-3 [Similar Products]
 UniProt Entry Name    MMP3_HUMAN
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 NCBI Summary for MMP3    Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MMP3    MMP3: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Defects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6). A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Protease; EC 3.4.24.17; Secreted

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; mitochondrion; extracellular region; cytosol

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: collagen catabolic process; positive regulation of protein oligomerization; extracellular matrix disassembly; extracellular matrix organization and biogenesis; negative regulation of protein kinase B signaling cascade; protein catabolic process; proteolysis; regulation of cell migration

Disease: Coronary Heart Disease, Susceptibility To, 6
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 Research Articles on MMP3    1. MMP-3 gene polymorphism had no predominant effect on the development of age-related macular degeneration in patients with myocardial infarction.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MMP3 recombinant proteinPathways associated with MMP3 recombinant protein
 Reference Product  PubMed Publications
 MMP1 recombinant protein  >223 publications with MMP3 and MMP1
 TIMP1 recombinant protein  >61 publications with MMP3 and TIMP1
 IL1B recombinant protein  >10 publications with MMP3 and IL1B
 CTNNB1 recombinant protein  >5 publications with MMP3 and CTNNB1
 EGFR recombinant protein  >5 publications with MMP3 and EGFR
 COL18A1 recombinant protein  >2 publications with MMP3 and COL18A1
 PLG recombinant protein  >2 publications with MMP3 and PLG
 BDNF recombinant protein  >1 publications with MMP3 and BDNF
 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Degradation Of Collagen Pathway antibodies  Degradation Of Collagen Pathway Diagram
 Degradation Of Collagen Pathway antibodies  Degradation Of Collagen Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 EGFR Transactivation By Gastrin Pathway antibodies  EGFR Transactivation By Gastrin Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
Diseases associated with MMP3 recombinant proteinOrgans/Tissues associated with MMP3 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >131 publications with MMP3 and Neoplasms
 Inflammation Antibodies  >124 publications with MMP3 and Inflammation
 Cardiovascular Diseases Antibodies  >85 publications with MMP3 and Cardiovascular Diseases
 Necrosis Antibodies  >71 publications with MMP3 and Necrosis
 Nervous System Diseases Antibodies  >58 publications with MMP3 and Nervous System Diseases
 Heart Diseases Antibodies  >41 publications with MMP3 and Heart Diseases
 Atherosclerosis Antibodies  >38 publications with MMP3 and Atherosclerosis
 Neoplasm Invasiveness Antibodies  >33 publications with MMP3 and Neoplasm Invasiveness
 Coronary Artery Disease Antibodies  >30 publications with MMP3 and Coronary Artery Disease
 Lung Diseases Antibodies  >30 publications with MMP3 and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >87 publications with MMP3 and Bone
 Connective Tissue Antibodies  >46 publications with MMP3 and Connective Tissue
 Muscle Antibodies  >40 publications with MMP3 and Muscle
 Lung Antibodies  >39 publications with MMP3 and Lung
 Brain Antibodies  >37 publications with MMP3 and Brain
 Skin Antibodies  >31 publications with MMP3 and Skin
 Eye Antibodies  >16 publications with MMP3 and Eye
 Uterus Antibodies  >13 publications with MMP3 and Uterus
 Placenta Antibodies  >12 publications with MMP3 and Placenta
 Intestine Antibodies  >10 publications with MMP3 and Intestine
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