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MUC16 recombinant protein :: Mucin 16 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS636801 MUC16 recombinant protein
Unit / Price
0.05 mg  /  $710 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Mucin 16 (MUC16), Recombinant Protein

★Popular Item★
 Also Known As   

Mucin 16, Recombinant, Human (CA125, FLJ14303, Ovarian Carcinoma Antigen CA 125)

 Product Gene Name   

MUC16 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 19; NC_000019.9 (8959516..9092018, complement). Location: 19p13.2
 OMIM    606154
 3D Structure    ModBase 3D Structure for Q8WXI7
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 Purity/Purification    Purified
90% as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: <1EU/ug protein (LAL)
 Form/Format    Supplied as a lyophilized powder from PBS, pH 7.4.
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 Source    Human Mucin 16 (Met13360-Gln 14347 (Met13472Thr & Gin 13957Lys) with a (-terminal 6-His tag, expressed in Chinese hamster ovary eCHO) cells (NP 078966.2)
 Biological Activity    Measured by its binding ability in a functional ELISA. When recombinant human MUC-16 is coated at 1ug/ml (100ul/well), the concentration of recombinant human Galectin-3 that produces 50% of the optimal binding response is found to be ~0.35-1.4ug/ml.
 Reconstitution    Reconstitute with 200ul sterile PBS
 SDS-PAGE    170-200kD, reducing conditions.
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 Preparation and Storage    Lyophilized powder may be stored at -20 degree C. Stable for 6 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of MUC16 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for MUC16 recombinant protein

   MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human MUC16 that can vary from 1148-22152aa in length and 200-5000kD in size. The 22152aa form contains ser/thr-rich N-terminal tandem repeats, 4 LRR (Leu-rich repeat) domains, 56 SEA (sea urchin sperm protein, enterokinase and agrin) domains, a transmembrane domain, and a 31aa cytoplasmic domain that includes a tyrosine phosphorylation site. SEA domains are ~120aa in length, contain conserved residues including potential O-glycosylation sites and a pair of cysteines, and are of ten found in transmembrane mucins. The protein represents aa21102-21992 of the full sequence and includes the last 6 SEA domains. It shares 68% aa identity with canine MUC16. MUC16 is overexpressed by tumor cells including ovarian and mesothelial cancers. The transmembrane form can adhere to mesothelinin the peritoneum, facilitating metastasis of ovarian cancer to the peritoneal cavity. MUC16 also binds galectin-1 on immune cells and enhances its expression on tumor cells. MUC16-expressing tumors adhere to NK cells, downregulate CD16 and suppress NK response, which may promote immune evasion. MUC16 is also cyclically expressed in the endometrium and may contribute to immune privilege during pregnancy. In the eye, MUC16 and other mucins protect the cornea and keep it hydrated. It is altered on the conjunctival epithelium of patients with non-Sjogren dry eye syndrome.
 Product Categories/Family for MUC16 recombinant protein    Molecular Biology; MB-Proteins
 Application Notes for MUC16 recombinant protein    SDS-PAGE: 170-200kD, reducing conditions.
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NCBI/Uniprot data below describe general gene information for MUC16. It may not necessarily be applicable to this product.
 NCBI GI #    83367077
 NCBI GeneID    94025
 NCBI Accession #    NP_078966.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_024690.2 [Other Products]
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 UniProt Primary Accession #    Q8WXI7 [Other Products]
 UniProt Secondary Accession #    Q6ZQW5; Q96RK2 [Other Products]
 UniProt Related Accession #    Q8WXI7 [Other Products]
 Molecular Weight    110kD [Similar Products]
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 NCBI Official Full Name    mucin-16
 NCBI Official Synonym Full Names    mucin 16, cell surface associated
 NCBI Official Symbol    MUC16 [Similar Products]
 NCBI Official Synonym Symbols   
CA125
[Similar Products]
 NCBI Protein Information    mucin-16; CA-125; MUC-16; CA125 ovarian cancer antigen; ovarian carcinoma antigen CA125; ovarian cancer-related tumor marker CA125
 UniProt Protein Name    Mucin-16
 UniProt Synonym Protein Names   
Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125
 Protein Family    Mucin
 UniProt Gene Name    MUC16 [Similar Products]
 UniProt Synonym Gene Names    CA125; MUC-16; CA-125 [Similar Products]
 UniProt Entry Name    MUC16_HUMAN
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 UniProt Comments for MUC16    MUC16: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; extrinsic to membrane; Golgi lumen; integral to membrane; plasma membrane; vesicle

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; cell adhesion; post-translational protein modification
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 Research Articles on MUC16    1. a high CA-125 level correlates significantly with many clinical features of advanced stage, poor prognostic factors, and worse TFS and OS. However, CA-125 is not an independent prognostic factor in patients with chronic lymphocytic leukemia.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MUC16 recombinant proteinPathways associated with MUC16 recombinant protein
 Reference Product  PubMed Publications
 MUC1 recombinant protein  >72 publications with MUC16 and MUC1
 MUC4 recombinant protein  >47 publications with MUC16 and MUC4
 MUC2 recombinant protein  >16 publications with MUC16 and MUC2
 MUC7 recombinant protein  >10 publications with MUC16 and MUC7
 MUC13 recombinant protein  >10 publications with MUC16 and MUC13
 MUC20 recombinant protein  >7 publications with MUC16 and MUC20
 MUC15 recombinant protein  >7 publications with MUC16 and MUC15
 LGALS1 recombinant protein  >3 publications with MUC16 and LGALS1
 MSLN recombinant protein  >3 publications with MUC16 and MSLN
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-linked Glycosylation Of Mucins Pathway antibodies  O-linked Glycosylation Of Mucins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Termination Of O-glycan Biosynthesis Pathway antibodies  Termination Of O-glycan Biosynthesis Pathway Diagram
Diseases associated with MUC16 recombinant proteinOrgans/Tissues associated with MUC16 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >219 publications with MUC16 and Neoplasms
 Ovarian Neoplasms Antibodies  >167 publications with MUC16 and Ovarian Neoplasms
 Adenocarcinoma Antibodies  >52 publications with MUC16 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >19 publications with MUC16 and Neoplasm Metastasis
 Endometrial Neoplasms Antibodies  >18 publications with MUC16 and Endometrial Neoplasms
 Inflammation Antibodies  >12 publications with MUC16 and Inflammation
 Breast Neoplasms Antibodies  >10 publications with MUC16 and Breast Neoplasms
 Carcinoma, Squamous Cell Antibodies  >5 publications with MUC16 and Carcinoma, Squamous Cell
 Fallopian Tube Neoplasms Antibodies  >5 publications with MUC16 and Fallopian Tube Neoplasms
 Uterine Cervical Neoplasms Antibodies  >5 publications with MUC16 and Uterine Cervical Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >172 publications with MUC16 and Blood
 Eye Antibodies  >52 publications with MUC16 and Eye
 Lung Antibodies  >29 publications with MUC16 and Lung
 Ovary Antibodies  >25 publications with MUC16 and Ovary
 Lymph Node Antibodies  >20 publications with MUC16 and Lymph Node
 Uterus Antibodies  >13 publications with MUC16 and Uterus
 Epididymis Antibodies  >11 publications with MUC16 and Epididymis
 Cervix Antibodies  >6 publications with MUC16 and Cervix
 Intestine Antibodies  >6 publications with MUC16 and Intestine
 Pancreas Antibodies  >5 publications with MUC16 and Pancreas
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