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| Product Name
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Mucin 16 (MUC16), Recombinant Protein ★Popular Item★
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| Also Known As
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Mucin 16, Recombinant, Human (CA125, FLJ14303, Ovarian Carcinoma Antigen CA 125)
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Chromosome Location
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Chromosome: 19; NC_000019.9 (8959516..9092018, complement). Location: 19p13.2
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| OMIM
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606154
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| 3D Structure
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ModBase 3D Structure for Q8WXI7
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| Purity/Purification
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Purified
90% as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: <1EU/ug protein (LAL)
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| Form/Format
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Supplied as a lyophilized powder from PBS, pH 7.4.
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| Source
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Human Mucin 16 (Met13360-Gln 14347 (Met13472Thr & Gin 13957Lys) with a (-terminal 6-His tag, expressed in Chinese hamster ovary eCHO) cells (NP 078966.2)
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| Biological Activity
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Measured by its binding ability in a functional ELISA. When recombinant human MUC-16 is coated at 1ug/ml (100ul/well), the concentration of recombinant human Galectin-3 that produces 50% of the optimal binding response is found to be ~0.35-1.4ug/ml.
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| Reconstitution
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Reconstitute with 200ul sterile PBS
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| SDS-PAGE
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170-200kD, reducing conditions.
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| Preparation and Storage
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Lyophilized powder may be stored at -20 degree C. Stable for 6 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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| Other Notes
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Small volumes of MUC16 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human MUC16 that can vary from 1148-22152aa in length and 200-5000kD in size. The 22152aa form contains ser/thr-rich N-terminal tandem repeats, 4 LRR (Leu-rich repeat) domains, 56 SEA (sea urchin sperm protein, enterokinase and agrin) domains, a transmembrane domain, and a 31aa cytoplasmic domain that includes a tyrosine phosphorylation site. SEA domains are ~120aa in length, contain conserved residues including potential O-glycosylation sites and a pair of cysteines, and are of ten found in transmembrane mucins. The protein represents aa21102-21992 of the full sequence and includes the last 6 SEA domains. It shares 68% aa identity with canine MUC16. MUC16 is overexpressed by tumor cells including ovarian and mesothelial cancers. The transmembrane form can adhere to mesothelinin the peritoneum, facilitating metastasis of ovarian cancer to the peritoneal cavity. MUC16 also binds galectin-1 on immune cells and enhances its expression on tumor cells. MUC16-expressing tumors adhere to NK cells, downregulate CD16 and suppress NK response, which may promote immune evasion. MUC16 is also cyclically expressed in the endometrium and may contribute to immune privilege during pregnancy. In the eye, MUC16 and other mucins protect the cornea and keep it hydrated. It is altered on the conjunctival epithelium of patients with non-Sjogren dry eye syndrome.
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| Product Categories/Family for MUC16 recombinant protein
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Molecular Biology; MB-Proteins
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| Application Notes for MUC16 recombinant protein
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SDS-PAGE: 170-200kD, reducing conditions.
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TOP
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| NCBI/Uniprot data below describe general gene information for MUC16. It may not necessarily be applicable to this product. |
| NCBI GI #
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83367077
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| NCBI GeneID
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94025
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| NCBI Accession #
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NP_078966.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_024690.2
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[Other Products]
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| UniProt Primary Accession #
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Q8WXI7
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[Other Products]
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| UniProt Secondary Accession #
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Q6ZQW5; Q96RK2
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[Other Products]
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| UniProt Related Accession #
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Q8WXI7
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[Other Products]
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| Molecular Weight
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110kD
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[Similar Products]
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TOP
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| NCBI Official Full Name
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mucin-16
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| NCBI Official Synonym Full Names
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mucin 16, cell surface associated
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| NCBI Official Symbol
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MUC16
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[Similar Products]
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| NCBI Official Synonym Symbols
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CA125
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[Similar Products]
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| NCBI Protein Information
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mucin-16; CA-125; MUC-16; CA125 ovarian cancer antigen; ovarian carcinoma antigen CA125; ovarian cancer-related tumor marker CA125
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| UniProt Protein Name
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Mucin-16
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| UniProt Synonym Protein Names
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Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125
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| Protein Family
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Mucin
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| UniProt Gene Name
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MUC16
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[Similar Products]
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| UniProt Synonym Gene Names
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CA125; MUC-16; CA-125
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[Similar Products]
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| UniProt Entry Name
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MUC16_HUMAN
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| UniProt Comments for MUC16
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MUC16: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, peripheral
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: extracellular space; extrinsic to membrane; Golgi lumen; integral to membrane; plasma membrane; vesicle
Molecular Function: protein binding
Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; cell adhesion; post-translational protein modification
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TOP
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| Research Articles on MUC16
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1. a high CA-125 level correlates significantly with many clinical features of advanced stage, poor prognostic factors, and worse TFS and OS. However, CA-125 is not an independent prognostic factor in patients with chronic lymphocytic leukemia.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with MUC16 recombinant protein | Pathways associated with MUC16 recombinant protein |
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| Diseases associated with MUC16 recombinant protein | Organs/Tissues associated with MUC16 recombinant protein |
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TOP
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Recombinant Protein 
