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CHRM3 recombinant protein :: Muscarinic acetylcholine receptor M3 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS955742
SDS-PAGE
Unit / Price
0.01 mg (E-Coli)  /  $150 +1 FREE 8GB USB
0.01 mg (Yeast)  /  $170 +1 FREE 8GB USB
0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $220 +1 FREE 8GB USB
0.1 mg (E-Coli)  /  $285 +1 FREE 8GB USB
0.1 mg (Yeast)  /  $345 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $545 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $900 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $930 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,170 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,170 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
1 mg (Yeast)  /  $1,410 +1 FREE 8GB USB
0.5 mg (Baculovirus)  /  $1,690 +2 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $1,780 +2 FREE 8GB USB
1 mg (Baculovirus)  /  $2,255 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Muscarinic acetylcholine receptor M3 (CHRM3), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human Muscarinic acetylcholine receptor M3

 Product Gene Name   

CHRM3 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    253-492. Partial.
 Sequence    RIYKETEKRT KELAGLQASG TEAETENFVH PTGSSRSCSS YELQQQSMKR SNRRKYGRCH FWFTTKSWKP SSEQMDQDHS SSDSWNNNDA AASLENSASS DEEDIGSETR AIYSIVLKLP GHSTILNSTK LPSSDNLQVP EEELGMVDLE RKADKLQAQK SVDDGGSFPK SFSKLPIQLE SAVDTAKTSD VNSSVGKSTA TLPLSFKEAT LAKRFALKTR SQITKRKRMS LVKEKKAAQT
 OMIM    100100
 3D Structure    ModBase 3D Structure for P20309
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of CHRM3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for CHRM3 recombinant protein

   The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
 Product Categories/Family for CHRM3 recombinant protein    Neuroscience
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 SDS-PAGE of CHRM3 recombinant protein    CHRM3 recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for CHRM3. It may not necessarily be applicable to this product.
 NCBI GI #    4502819
 NCBI GeneID    1131
 NCBI Accession #    NP_000731.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000740.2 [Other Products]
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 UniProt Primary Accession #    P20309 [Other Products]
 UniProt Secondary Accession #    Q0VAJ8; Q4QRI3; Q5VXY2; Q9HB60 [Other Products]
 UniProt Related Accession #    P20309 [Other Products]
 Molecular Weight    28.6kD
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 NCBI Official Full Name    muscarinic acetylcholine receptor M3
 NCBI Official Synonym Full Names    cholinergic receptor, muscarinic 3
 NCBI Official Symbol    CHRM3 [Similar Products]
 NCBI Official Synonym Symbols   
HM3; PBS; EGBRS
[Similar Products]
 NCBI Protein Information    muscarinic acetylcholine receptor M3
 UniProt Protein Name    Muscarinic acetylcholine receptor M3
 Protein Family    Muscarinic acetylcholine receptor
 UniProt Gene Name    CHRM3 [Similar Products]
 UniProt Entry Name    ACM3_HUMAN
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 NCBI Summary for CHRM3    The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CHRM3    mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: asymmetric synapse; basolateral plasma membrane; cell junction; dendrite; integral to plasma membrane; nerve terminal; plasma membrane; postsynaptic membrane; synapse

Molecular Function: acetylcholine binding; drug binding; G-protein coupled acetylcholine receptor activity; phosphoinositide phospholipase C activity; protein binding; receptor activity

Biological Process: acetylcholine receptor signaling, muscarinic pathway; cell proliferation; energy reserve metabolic process; G-protein coupled receptor protein signaling pathway; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; muscarinic acetylcholine receptor, phospholipase C activating pathway; nervous system development; positive regulation of smooth muscle contraction; protein modification process; regulation of insulin secretion; regulation of vascular smooth muscle contraction; saliva secretion; signal transduction; smooth muscle contraction; synaptic transmission, cholinergic

Disease: Abdominal Muscles, Absence Of, With Urinary Tract Abnormality And Cryptorchidism
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Product References and Citations for CHRM3 recombinant protein

   Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors.Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J., Capon D.J.EMBO J. 6:3923-3929(1987) Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes.Bonner T.I., Young A.C., Brann M.R., Buckley N.J.Neuron 1:403-410(1988) Human-specific amino acid changes found in 103 protein-coding genes.Kitano T., Liu Y.-H., Ueda S., Saitou N.Mol. Biol. Evol. 21:936-944(2004) cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org) .Puhl H.L. III, Ikeda S.R., Aronstam R.S. The DNA sequence and biological annotation of human chromosome 1.Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.Nature 441:315-321(2006) Antisense promoter of human L1 retrotransposon drives transcription of adjacent cellular genes.Speek M.Mol. Cell. Biol. 21:1973-1985(2001) Mutation of carboxyl-terminal threonine residues in human M3 muscarinic acetylcholine receptor modulates the extent of sequestration and desensitization.Yang J., Williams J.A., Yule D.I., Logsdon C.D.Mol. Pharmacol. 48:477-485(1995) Muscarinic acetylcholine receptor M3 mutation causes urinary bladder disease and a prune-belly-like syndrome.Weber S., Thiele H., Mir S., Toliat M.R., Sozeri B., Reutter H., Draaken M., Ludwig M., Altmuller J., Frommolt P., Stuart H.M., Ranjzad P., Hanley N.A., Jennings R., Newman W.G., Wilcox D.T., Thiel U., Schlingmann K.P., Beetz R., Hoyer P.F., Konrad M., Schaefer F., Nurnberg P., Woolf A.S.Am. J. Hum. Genet. 89:668-674(2011) The muscarinic M3 acetylcholine receptor exists as two differently sized complexes at the plasma membrane.Patowary S., Alvarez-Curto E., Xu T.R., Holz J.D., Oliver J.A., Milligan G., Raicu V.Biochem. J. 452:303-312(2013) Identification and structural determination of the M(3) muscarinic acetylcholine receptor basolateral sorting signal.Iverson H.A., Fox D. III, Nadler L.S., Klevit R.E., Nathanson N.M.J. Biol. Chem. 280:24568-24575(2005)
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 Research Articles on CHRM3    1. Cigarette smoking may contribute to this imbalance by affecting the polarization and survival of Th/Tregs through the up-regulation of MR3 and MR5.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CHRM3 recombinant proteinDiseases associated with CHRM3 recombinant protein
 Products by Pathway  Pathway Diagram
 Acetylcholine Regulates Insulin Secretion Pathway antibodies  Acetylcholine Regulates Insulin Secretion Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >7 publications with CHRM3 and Nervous System Diseases
 Inflammation Antibodies  >5 publications with CHRM3 and Inflammation
 Disease Models, Animal Antibodies  >3 publications with CHRM3 and Disease Models, Animal
 Seizures Antibodies  >2 publications with CHRM3 and Seizures
 Pain Antibodies  >2 publications with CHRM3 and Pain
 Prune Belly Syndrome Antibodies  >2 publications with CHRM3 and Prune Belly Syndrome
 Depressive Disorder Antibodies  >2 publications with CHRM3 and Depressive Disorder
 Liver Neoplasms Antibodies  >2 publications with CHRM3 and Liver Neoplasms
 Drug-Related Side Effects and Adverse Reactions Antibodies  >2 publications with CHRM3 and Drug-Related Side Effects and Adverse Reactions
 Fibrosis Antibodies  >2 publications with CHRM3 and Fibrosis
Organs/Tissues associated with CHRM3 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >12 publications with CHRM3 and Brain
 Eye Antibodies  >3 publications with CHRM3 and Eye
 Heart Antibodies  >2 publications with CHRM3 and Heart
 Prostate Antibodies  >2 publications with CHRM3 and Prostate
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