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NANS recombinant protein

Scan QR to view Datasheet Catalog #    MBS203797
SDS-Page
Unit / Price
0.1 mg  /  $310 +1 FREE 8GB USB
0.5 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NANS, Recombinant Protein

 Also Known As   

NANS, 1-359aa, Human, His tag, E Coli

 Product Synonym Names    Sialic acid synthase; SAS; N acetylneuraminate synthase
 Product Gene Name   

NANS recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MPLELELCPG RWVGGQHPCF IIAEIGQNHQ GDLDVAKRMI RMAKECGADC AKFQKSELEF KFNRKALERP YTSKHSWGKT YGEHKRHLEF SHDQYRELQR YAEEVGIFFT ASGMDEMAVE FLHELNVPFF KVGSGDTNNF PYLEKTAKKG RPMVISSGMQ SMDTMKQVYQ IVKPLNPNFC FLQCTSAYPL QPEDVNLRVI SEYQKLFPDI PIGYSGHETG IAISVAAVAL GAKVLERHIT LDKTWKGSDH SASLEPGELA ELVRSVRLVE RALGSPTKQL LPCEMACNEK LGKSVVAKVK IPEGTILTMD MLTVKVGEPK GYPPEDIFNL VGKKVLVTVE EDDTIMEELV DNHGKKIKS
 OMIM    AF257466 mRNA
 3D Structure    ModBase 3D Structure for Q9NR45
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 Host    E Coli
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 Purity/Purification    > 95% by SDS-PAGE
 Form/Format    Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
 Concentration    0.5 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag Information    His-tag
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of NANS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for NANS recombinant protein

   NANS, also known as SAS, is a 359 amino acid protein that contains one AFP (antifreeze proteins)-like domain and functions in the biosynthesis of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis. Recombinant human NANS protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
 Product Categories/Family for NANS recombinant protein    Metabolism
 Applications Tested/Suitable for NANS recombinant protein   

SDS-PAGE

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 SDS-Page of NANS recombinant protein    NANS recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for NANS. It may not necessarily be applicable to this product.
 NCBI GI #    12056473
 NCBI GeneID    54187
 NCBI Accession #    NP_061819 [Other Products]
 NCBI GenBank Nucleotide #    NM_018946.3 [Other Products]
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 UniProt Primary Accession #    Q9NR45 [Other Products]
 UniProt Secondary Accession #    Q8WUV9; Q9BWS6; Q9NVD4; B2RE98 [Other Products]
 UniProt Related Accession #    Q9NR45 [Other Products]
 Molecular Weight    42.4 kDa (379aa) confirmed by MALDI-TOF
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 NCBI Official Full Name    sialic acid synthase
 NCBI Official Synonym Full Names    N-acetylneuraminate synthase
 NCBI Official Symbol    NANS [Similar Products]
 NCBI Official Synonym Symbols   
SAS; SEMDG; SEMDCG; HEL-S-100
[Similar Products]
 NCBI Protein Information    sialic acid synthase
 UniProt Protein Name    Sialic acid synthase
 UniProt Synonym Protein Names   
N-acetylneuraminate synthase (EC:2.5.1.56); N-acetylneuraminate-9-phosphate synthase (EC:2.5.1.57); N-acetylneuraminic acid phosphate synthase; N-acetylneuraminic acid synthase
 Protein Family    Sialic acid synthase
 UniProt Gene Name    NANS [Similar Products]
 UniProt Synonym Gene Names    SAS [Similar Products]
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 NCBI Summary for NANS    This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008]
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 UniProt Comments for NANS    Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.
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Product References and Citations for NANS recombinant protein

   Milstein O., et al. (2008) J Biol Chem. 283 (49):34414-22.; Aldape MJ., et al. (2007) J Infect Dis. 195 (12):1838-45.;
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 Research Articles on NANS    1. NANS deficiency is associated with defect in brain and skeletal development.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NANS recombinant proteinPathways associated with NANS recombinant protein
 Reference Product  PubMed Publications
 GNE recombinant protein  >1 publications with NANS and GNE
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway antibodies  Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway Diagram
 CMP-N-acetylneuraminate Biosynthesis I (eukaryotes) Pathway antibodies  CMP-N-acetylneuraminate Biosynthesis I (eukaryotes) Pathway Diagram
 CMP-N-acetylneuraminate Biosynthesis I (eukaryotes) Pathway antibodies  CMP-N-acetylneuraminate Biosynthesis I (eukaryotes) Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Sialic Acid Metabolism Pathway antibodies  Sialic Acid Metabolism Pathway Diagram
Diseases associated with NANS recombinant proteinOrgans/Tissues associated with NANS recombinant protein
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >2 publications with NANS and Cardiovascular Diseases
 Kidney Diseases Antibodies  >2 publications with NANS and Kidney Diseases
 Heart Diseases Antibodies  >1 publications with NANS and Heart Diseases
 Weight Gain Antibodies  >1 publications with NANS and Weight Gain
 Hyperplasia Antibodies  >1 publications with NANS and Hyperplasia
 Developmental Disabilities Antibodies  >1 publications with NANS and Developmental Disabilities
 Bone Diseases, Developmental Antibodies  >1 publications with NANS and Bone Diseases, Developmental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7 publications with NANS and Blood
 Brain Antibodies  >4 publications with NANS and Brain
 Kidney Antibodies  >3 publications with NANS and Kidney
 Skin Antibodies  >3 publications with NANS and Skin
 Bone Antibodies  >2 publications with NANS and Bone
 Ovary Antibodies  >2 publications with NANS and Ovary
 Eye Antibodies  >1 publications with NANS and Eye
 Intestine Antibodies  >1 publications with NANS and Intestine
 Prostate Antibodies  >1 publications with NANS and Prostate
 Muscle Antibodies  >1 publications with NANS and Muscle
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