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NEDD8 recombinant protein

Scan QR to view Datasheet Catalog #    MBS203213
SDS-Page
Unit / Price
0.1 mg  /  $310 +1 FREE 8GB USB
0.5 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NEDD8, Recombinant Protein

 Also Known As   

NEDD8, 1-76aa, Human, His tag, E Coli

 Product Synonym Names    Neural precursor cell expressed developmentally down-regulated 8; ubiquitin-like protein Nedd8; Neddylin; Neural precursor cell expressed; developmentally down-regulated 8 MGC104393; MGC125896; MGC125897; NED8; 0NEDD 8; Neddylin; Neural precursor cell expressed developmentally down regulated 8; Neural precursor cell expressed developmentally down regulated gene 8; Rub1; ubiquitin like protein Nedd 8; ubiquitin like protein Nedd8.
 Product Gene Name   

NEDD8 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE KTAADYKILG GSVLHLVLAL RGG
 OMIM    D23662 mRNA
 3D Structure    ModBase 3D Structure for Q15843
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 Host    E Coli
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 Purity/Purification    > 95% by SDS-PAGE
 Form/Format    In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.5mM DTT, 50mM NaCl, 10% glycerol
 Concentration    0.5 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag Information    His-tag
 Endotoxin Level    < 1.0 EU per 1 microgram of protein (determined by LAL method)
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of NEDD8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for NEDD8 recombinant protein

   NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E Coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.
 Product Categories/Family for NEDD8 recombinant protein    ubiquitination
 Applications Tested/Suitable for NEDD8 recombinant protein   

SDS-PAGE

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 SDS-Page of NEDD8 recombinant protein    NEDD8 recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for NEDD8. It may not necessarily be applicable to this product.
 NCBI GI #    5453760
 NCBI GeneID    4738
 NCBI Accession #    NP_006147 [Other Products]
 NCBI GenBank Nucleotide #    NM_006156.2 [Other Products]
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 UniProt Primary Accession #    Q15843 [Other Products]
 UniProt Secondary Accession #    Q3SXN8; Q6LES6 [Other Products]
 UniProt Related Accession #    Q15843 [Other Products]
 Molecular Weight    12.8 kDa (113 aa), confirmed by MALDI-TOF.
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 NCBI Official Full Name    NEDD8
 NCBI Official Synonym Full Names    neural precursor cell expressed, developmentally down-regulated 8
 NCBI Official Symbol    NEDD8 [Similar Products]
 NCBI Official Synonym Symbols   
NEDD-8
[Similar Products]
 NCBI Protein Information    NEDD8
 UniProt Protein Name    NEDD8
 UniProt Synonym Protein Names   
Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8
 Protein Family    NEDD8
 UniProt Gene Name    NEDD8 [Similar Products]
 UniProt Synonym Gene Names    NEDD-8 [Similar Products]
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 UniProt Comments for NEDD8    Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
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Product References and Citations for NEDD8 recombinant protein

   Wu K., et al. (2002) J Biol Chem. 277 (1): 516-27; Hori T., et al. (1999) Oncogene. 18 (48): 6829-34;
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 Research Articles on NEDD8    1. Furthermore, the authors characterized SENP8/DEN1 as the protease that counteracts Ubc12 auto-neddylation, and observed aberrant neddylation of Ubc12 and other NEDD8 conjugation pathway components in SENP8-deficient cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NEDD8 recombinant proteinPathways associated with NEDD8 recombinant protein
 Reference Product  PubMed Publications
 CUL1 recombinant protein  >70 publications with NEDD8 and CUL1
 UBA3 recombinant protein  >61 publications with NEDD8 and UBA3
 RBX1 recombinant protein  >59 publications with NEDD8 and RBX1
 SKP1 recombinant protein  >57 publications with NEDD8 and SKP1
 SENP8 recombinant protein  >25 publications with NEDD8 and SENP8
 NAE1 recombinant protein  >15 publications with NEDD8 and NAE1
 COPS5 recombinant protein  >12 publications with NEDD8 and COPS5
 UBE2M recombinant protein  >11 publications with NEDD8 and UBE2M
 UCHL3 recombinant protein  >3 publications with NEDD8 and UCHL3
 COPS6 recombinant protein  >2 publications with NEDD8 and COPS6
 Products by Pathway  Pathway Diagram
 Cargo Recognition For Clathrin-mediated Endocytosis Pathway antibodies  Cargo Recognition For Clathrin-mediated Endocytosis Pathway Diagram
 Clathrin-mediated Endocytosis Pathway antibodies  Clathrin-mediated Endocytosis Pathway Diagram
 Deubiquitination Pathway antibodies  Deubiquitination Pathway Diagram
 EGF/EGFR Signaling Pathway antibodies  EGF/EGFR Signaling Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By TGF-beta Receptor Complex Pathway antibodies  Signaling By TGF-beta Receptor Complex Pathway Diagram
 TGF-beta Receptor Signaling Activates SMADs Pathway antibodies  TGF-beta Receptor Signaling Activates SMADs Pathway Diagram
Diseases associated with NEDD8 recombinant proteinOrgans/Tissues associated with NEDD8 recombinant protein
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >22 publications with NEDD8 and Adenocarcinoma
 Inflammation Antibodies  >19 publications with NEDD8 and Inflammation
 Necrosis Antibodies  >14 publications with NEDD8 and Necrosis
 Liver Diseases Antibodies  >12 publications with NEDD8 and Liver Diseases
 Liver Neoplasms Antibodies  >9 publications with NEDD8 and Liver Neoplasms
 Kidney Diseases Antibodies  >8 publications with NEDD8 and Kidney Diseases
 Neoplasms, Experimental Antibodies  >8 publications with NEDD8 and Neoplasms, Experimental
 Anemia Antibodies  >7 publications with NEDD8 and Anemia
 Breast Neoplasms Antibodies  >7 publications with NEDD8 and Breast Neoplasms
 Lung Neoplasms Antibodies  >6 publications with NEDD8 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >40 publications with NEDD8 and Blood
 Brain Antibodies  >36 publications with NEDD8 and Brain
 Vascular Antibodies  >22 publications with NEDD8 and Vascular
 Kidney Antibodies  >20 publications with NEDD8 and Kidney
 Lung Antibodies  >19 publications with NEDD8 and Lung
 Liver Antibodies  >16 publications with NEDD8 and Liver
 Muscle Antibodies  >15 publications with NEDD8 and Muscle
 Nerve Antibodies  >13 publications with NEDD8 and Nerve
 Bone Antibodies  >13 publications with NEDD8 and Bone
 Heart Antibodies  >12 publications with NEDD8 and Heart
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