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NFNB recombinant protein

Scan QR to view Datasheet Catalog #    MBS203581
SDS-PAGE
Unit / Price
0.1 mg  /  $310 +1 FREE 8GB USB
0.5 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NFNB, Recombinant Protein

 Also Known As   

NFNB, 1-217aa, E Coli, His tag, E Coli

 Product Synonym Names    dprA; nfsB; nfsI; ntr; dihydropteridine reductase; NAD(P)H-dependent; oxygen-insensitive
 Product Gene Name   

NFNB recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV
 3D Structure    ModBase 3D Structure for P38489
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 Purity/Purification    > 95% by SDS - PAGE
 Form/Format    Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM Nacl, 1mM DTT, 10% glycerol
 Concentration    1 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    E Coli
 Tag    His-tag
 Expression System    E Coli
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of NFNB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for NFNB recombinant protein

   NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
 Product Categories/Family for NFNB recombinant protein    Redox Proteins
 Applications Tested/Suitable for NFNB recombinant protein   

SDS-PAGE

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 SDS-PAGE of NFNB recombinant protein    NFNB recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for NFNB. It may not necessarily be applicable to this product.
 NCBI GI #    16128561
 NCBI GeneID    945778
 NCBI Accession #    NP_415110 [Other Products]
 NCBI GenBank Nucleotide #    NC_000913.3 [Other Products]
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 UniProt Primary Accession #    P38489 [Other Products]
 UniProt Secondary Accession #    P19575 [Other Products]
 Molecular Weight    26.0 kDa (237aa) confirmed by MALDI-TOF
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 NCBI Official Full Name    dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive
 NCBI Official Symbol    nfsB [Similar Products]
 NCBI Official Synonym Symbols   
dprA; ECK0570; JW0567; nfnB; nfsI; ntr
[Similar Products]
 NCBI Protein Information    dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive
 UniProt Protein Name    Oxygen-insensitive NAD(P)H nitroreductase
 UniProt Synonym Protein Names   
Dihydropteridine reductase (EC:1.5.1.34); FMN-dependent nitroreductase
 Protein Family    Nitroreductase
 UniProt Gene Name    nfnB [Similar Products]
 UniProt Synonym Gene Names    dprA; nfsB; nfsI; ntr [Similar Products]
 UniProt Entry Name    NFNB_ECOLI
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 NCBI Summary for NFNB    nfsA, nfsB, rutE, and ydjA are paralogs. [More information is available at EcoGene: EG20151]. Aspartate 37 has been implicated in binding the NADH cofactor. [More information is available at EcoCyc: EG50005].
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 UniProt Comments for NFNB    Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.
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Product References and Citations for NFNB recombinant protein

   Jarrom D., et al. (2009) Biochemistry. 48(32):7665-72.
Singleton DC., et al. (2007) Cancer Gene Ther. 14(12):953-67.
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 Research Articles on NFNB    1. NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NFNB recombinant proteinPathways associated with NFNB recombinant protein
 Reference Product  PubMed Publications
 nfsA recombinant protein  >23 publications with NFNB and nfsA
 nemA recombinant protein  >4 publications with NFNB and nemA
 mdaB recombinant protein  >2 publications with NFNB and mdaB
 Products by Pathway  Pathway Diagram
 Microbial Metabolism In Diverse Environments Pathway antibodies  Microbial Metabolism In Diverse Environments Pathway Diagram
 Nitrotoluene Degradation Pathway antibodies  Nitrotoluene Degradation Pathway Diagram
 Nitrotoluene Degradation Pathway antibodies  Nitrotoluene Degradation Pathway Diagram
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