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NS1 recombinant protein

Scan QR to view Datasheet Catalog #    MBS505008
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 Product Name   

NS1, Recombinant Protein

 Also Known As   

NS1 recombinant

 Product Synonym Names    RECOMBINANT ANTIGEN NS1 from Dengue Virus serotype 4; non-structiral 1 protein
 Product Gene Name   

NS1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Host    E Coli
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 Purity/Purification    0.95
 Form/Format    Liquid protein solution
 Concentration    0.679 mg/ml (lot specific)
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 Recombinant Antigen    Dengue antigen NS1 (Libraty et al., 2002).
 Organism    DENV4
 Tag    HIS
 Molecular Weight Information    Determined by SDS-PAGE, theprotein band is between molecular markers of 66,200-45,000 Da, while relative molecular mass calculated fromamino acid sequence is 47,578.7 Da.
 Observation Notes    Proteins should be maintained frozenat high concentrations. The dilution to be performed forELISA assays should be made with a small quantity ofprotein, the same day of the experiment. In order to defrostthe protein, maintain the aliquot at 25 degree C without shaking toavoid aggregation. Prior making test dilutions and afterdefrost the protein, is recommended to remove possibleprotein aggregates by centrifuging the stock solution,avoiding alterations in the immobilization of the biomoleculeto the solid surface.
 Buffer    20 mM phosphate buffer pH 8, 1 M NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 6 M urea
 Batch Composition    Recombinant antigen with a his-tag in its N-terminus
 Storage Buffer    20 mM phosphate buffer pH 8, 1 M NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 6 M urea
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 Preparation and Storage    Protein is shipped with dry ice. Upon arrival,it should be aliquoted in order to avoid repeated freezingand thawing cycles and stored at -20 degree C to -80 degree C
 Other Notes    Small volumes of NS1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for NS1 recombinant protein

   the non structural antigen NS1 has beenprepared as a recombinant antigen fused to a his-tag at theN-terminal. It is produced from the complete ORF of theviral-encoded 46 kilodalton glycoprotein gp46.
 Product Categories/Family for NS1 recombinant protein    VIRUSES
 Applications Tested/Suitable for NS1 recombinant protein   

ELISA (EIA), Western Blot (WB)

 Application Notes for NS1 recombinant protein    Recommended concentration in ELISA plates: 0.9 ug/ml in plates for IgG and IgM detection
Suggested Titer by ELISA: 1:750, which corresponds to 0.9 ug/ml in plates for IgG and IgM detection.
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 Testing Data of NS1 recombinant protein    NS1 recombinant protein Testing Data image
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 Testing Data #2 of NS1 recombinant protein    NS1 recombinant protein Testing Data #2 image
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 Testing Data #3 of NS1 recombinant protein    NS1 recombinant protein Testing Data #3 image
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NCBI/Uniprot data below describe general gene information for NS1. It may not necessarily be applicable to this product.
 NCBI GI #    259496218
 NCBI Accession #    GQ868594.1 [Other Products]
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 NCBI Official Full Name    Dengue virus 4 isolate DENV-4/PH/BID-V3361/1956, complete genome
 Protein Family    Non-capsid protein
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Product References and Citations for NS1 recombinant protein

   Libraty DH et al., 2002. High circulating levels of thedengue virus nonstructural protein NS1 early in dengueillness correlate with the development of denguehemorrhagic fever. The Journal of Infectious Diseases,186:1165-1168.
Gill SC, von Hippel PH. Calculation of protein extinctioncoefficients from amino acid sequence data. AnalBiochem. 1989 Nov 1;182(2):319-26.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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