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ELANE recombinant protein :: Neutrophil elastase Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS957715
Unit / Price
0.01 mg (E-Coli)  /  $150 +1 FREE 8GB USB
0.01 mg (Yeast)  /  $170 +1 FREE 8GB USB
0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $220 +1 FREE 8GB USB
0.1 mg (E-Coli)  /  $285 +1 FREE 8GB USB
0.1 mg (Yeast)  /  $345 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $545 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $900 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $930 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,170 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,170 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
1 mg (Yeast)  /  $1,410 +1 FREE 8GB USB
0.5 mg (Baculovirus)  /  $1,690 +2 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $1,775 +2 FREE 8GB USB
1 mg (Baculovirus)  /  $2,255 +2 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Neutrophil elastase (ELANE), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human Neutrophil elastase (ELANE)

 Product Synonym Names    Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE: Medullasin; PMN elastase
 Product Gene Name   

ELANE recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 MBS957715 COA    COA PDF
 Sequence Positions    30-267. Full length of the mature protein.
 OMIM    130130
 3D Structure    ModBase 3D Structure for P08246
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Production Note    Special Offer: The E. coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E. coli host-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E. coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E. coli host-expressed protein for the fastest delivery among all hosts. Please contact us or email to for more details.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of ELANE recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ELANE recombinant protein

   Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
 Product Categories/Family for ELANE recombinant protein    Signal Transduction
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 SDS-Page of ELANE recombinant protein    ELANE recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for ELANE. It may not necessarily be applicable to this product.
 NCBI GI #    4503549
 NCBI GeneID    1991
 NCBI Accession #    NP_001963.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001972.3 [Other Products]
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 UniProt Primary Accession #    P08246 [Other Products]
 UniProt Secondary Accession #    P09649; Q6B0D9; Q6LDP5 [Other Products]
 UniProt Related Accession #    P08246 [Other Products]
 Molecular Weight    52.9kD
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 NCBI Official Full Name    neutrophil elastase preproprotein
 NCBI Official Synonym Full Names    elastase, neutrophil expressed
 NCBI Official Symbol    ELANE [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    neutrophil elastase
 UniProt Protein Name    Neutrophil elastase
 UniProt Synonym Protein Names   
Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase
 Protein Family    Neutrophil elastase
 UniProt Gene Name    ELANE [Similar Products]
 UniProt Synonym Gene Names    ELA2; HLE [Similar Products]
 UniProt Entry Name    ELNE_HUMAN
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 NCBI Summary for ELANE    Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]
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 UniProt Comments for ELANE    ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Cell cycle regulation; Protease; Motility/polarity/chemotaxis; EC; Cell surface

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; secretory granule; transcriptional repressor complex

Molecular Function: cytokine binding; endopeptidase activity; heparin binding; peptidase activity; protease binding; protein binding; serine-type endopeptidase activity

Biological Process: acute inflammatory response to antigenic stimulus; cellular calcium ion homeostasis; collagen catabolic process; defense response to bacterium; extracellular matrix disassembly; extracellular matrix organization and biogenesis; leukocyte migration; negative regulation of chemokine biosynthetic process; negative regulation of chemotaxis; negative regulation of inflammatory response; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; phagocytosis; positive regulation of immune response; positive regulation of interleukin-8 biosynthetic process; positive regulation of MAP kinase activity; positive regulation of smooth muscle cell proliferation; protein catabolic process; proteolysis; response to lipopolysaccharide; response to UV; response to yeast

Disease: Cyclic Neutropenia; Neutropenia, Severe Congenital, 1, Autosomal Dominant
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Product References and Citations for ELANE recombinant protein

   Nucleotide sequence of human bone marrow serine protease (medullasin) gene.Nakamura H., Okano K., Aoki Y., Shimizu H., Naruto M.Nucleic Acids Res. 15:9601-9601(1987) Structure of the human neutrophil elastase gene.Takahashi H., Nukiwa T., Yoshimura K., Quick C.D., States D.J., Holmes M.D., Whang-Peng J., Knutsen T., Crystal R.G.J. Biol. Chem. 263:14739-14747(1988) The human neutrophil elastase gene. Analysis of the nucleotide sequence reveals three distinct classes of repetitive DNA.Farley D., Travis J., Salvesen G.Biol. Chem. Hoppe-Seyler 370:737-744(1989) Functional expression of human leukocyte elastase (HLE) /medullasin in eukaryotic cells.Okano K., Aoki Y., Shimizu H., Naruto M.Biochem. Biophys. Res. Commun. 167:1326-1332(1990) NIEHS SNPs program Molecular cloning of complementary DNA for human medullasin an inflammatory serine protease in bone marrow cells.Okano K., Aoki Y., Sakurai T., Kajitani M., Kanai S., Shimazu T., Shimizu H., Naruto M.J. Biochem. 102:13-16(1987) Primary structure of human neutrophil elastase.Sinha S., Watorek W., Karr S., Giles J., Bode W., Travis J.Proc. Natl. Acad. Sci. U.S.A. 84:2228-2232(1987) Travis J., Giles P.J., Porcelli L., Reilly C.F., Baugh R., Powers J.(In) Protein degradation in health and disease, Ciba Foundation Symposium, pp.75:51-68, Excerpta Medica, Amsterdam and Oxford (1980) Antibiotic proteins of human polymorphonuclear leukocytes.Gabay J.E., Scott R.W., Campanelli D., Griffith J., Wilde C., Marra M.N., Seeger M., Nathan C.F.Proc. Natl. Acad. Sci. U.S.A. 86:5610-5614(1989) Use of proteinase 3 purified by reverse phase HPLC to detect autoantibodies in systemic vasculitis.Gaskin G., Kendal H., Coulthart A., Turner N., Pusey C.D.J. Immunol. Methods 180:25-33(1995) Myelomonocytic cell lineage expression of the neutrophil elastase gene.Takahashi H., Nukiwa T., Basset P., Cystal R.G.J. Biol. Chem. 263:2543-2547(1988) Molecular cloning of human neutrophil elastase.Farley D., Salvesen G.S., Travis J.Biol. Chem. Hoppe-Seyler 369:3-7(1988) The primary structure and elastinolytic activity of medullasin (a serine protease of bone marrow) .Aoki Y., Hase T.Biochem. Biophys. Res. Commun. 178:501-506(1991) Human leukocyte elastase and cathepsin G are specific inhibitors of C5a-dependent neutrophil enzyme release and chemotaxis.Tralau T., Meyer-Hoffert U., Schroder J.M., Wiedow O.Exp. Dermatol. 13:316-325(2004) A novel notch protein, N2N, targeted by neutrophil elastase and implicated in hereditary neutropenia.Duan Z., Li F.-Q., Wechsler J., Meade-White K., Williams K., Benson K.F., Horwitz M.Mol. Cell. Biol. 24:58-70(2004) Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.J. Proteome Res. 8:651-661(2009) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) Structure of human neutrophil elastase in complex with a peptide chloromethyl ketone inhibitor at 1.84-A resolution.Navia M.A., McKeever B.M., Springer J.P., Lin T.-Y., Williams H.R., Fluder E.M., Dorn C.P., Hoogsteen K.Proc. Natl. Acad. Sci. U.S.A. 86:7-11(1989) The refined 2.3-A crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor.Wei A.-Z., Mayr I., Bode W.FEBS Lett. 234:367-373(1988) X-ray crystal structure of the complex of human leukocyte elastase (PMN elastase) and the third domain of the turkey ovomucoid inhibitor.Bode W., Wei A.-Z., Huber R., Meyer E., Travis J., Neumann S.EMBO J. 5:2453-2458(1986) Mutations in ELA2, encoding neutrophil elastase, define a 21-day biological clock in cyclic haematopoiesis.Horwitz M., Benson K.F., Person R.E., Aprikyan A.G., Dale D.C.Nat. Genet. 23:433-436(1999) Mutations in the gene encoding neutrophil elastase in congenital and cyclic neutropenia.Dale D.C., Person R.E., Bolyard A.A., Aprikyan A.G., Bos C., Bonilla M.A., Boxer L.A., Kannourakis G., Zeidler C., Welte K., Benson K.F., Horwitz M.Blood 96:2317-2322(2000) Mutations in the ELA2 gene encoding neutrophil elastase are present in most patients with sporadic severe congenital neutropenia but only in some patients with the familial form of the disease.Ancliff P.J., Gale R.E., Liesner R., Hann I.M., Linch D.C.Blood 98:2645-2650(2001) Paternal mosaicism proves the pathogenic nature of mutations in neutrophil elastase in severe congenital neutropenia.Ancliff P.J., Gale R.E., Watts M.J., Liesner R., Hann I.M., Strobel S., Linch D.C.Blood 100:707-709(2002) Mutations in the ELA2 gene correlate with more severe expression of neutropenia a study of 81 patients from the French Neutropenia Register.Bellanne-Chantelot C., Clauin S., Leblanc T., Cassinat B., Rodrigues-Lima F., Beaufils S., Vaury C., Barkaoui M., Fenneteau O., Maier-Redelsperger M., Chomienne C., Donadieu J.Blood 103:4119-4125(2004) Double de novo mutations of ELA2 in cyclic and severe congenital neutropenia.Salipante S.J., Benson K.F., Luty J., Hadavi V., Kariminejad R., Kariminejad M.H., Rezaei N., Horwitz M.S.Hum. Mutat. 28:874-881(2007) A novel mutation Ala57Val of the ELA2 gene in a Korean boy with severe congenital neutropenia.Lee S.T., Yoon H.S., Kim H.J., Lee J.H., Park J.H., Kim S.H., Seo J.J., Im H.J.Ann. Hematol. 88:593-595(2009) Homozygous HAX1 mutations in severe congenital neutropenia patients with sporadic disease a novel mutation in two unrelated British kindreds.Smith B.N., Ancliff P.J., Pizzey A., Khwaja A., Linch D.C., Gale R.E.Br. J. Haematol. 144:762-770(2009) Ela2 mutations and clinical manifestations in familial congenital neutropenia.Shiohara M., Shigemura T., Saito S., Tanaka M., Yanagisawa R., Sakashita K., Asada H., Ishii E., Koike K., Chin M., Kobayashi M., Koike K.J. Pediatr. Hematol. Oncol. 31:319-324(2009) Digenic mutations in severe congenital neutropenia.Germeshausen M., Zeidler C., Stuhrmann M., Lanciotti M., Ballmaier M., Welte K.Haematologica 95:1207-1210(2010) Pegfilgrastim in children with severe congenital neutropenia.Fioredda F., Calvillo M., Lanciotti M., Lanza T., Giunti L., Castagnola E., Lorenzi I., Tonelli R., Ghezzi P., Dufour C.Pediatr. Blood Cancer 54:465-467(2010) Severe congenital neutropenia in a multigenerational family with a novel neutrophil elastase (ELANE) mutation.van de Vosse E., Verhard E.M., Tool A.J., de Visser A.W., Kuijpers T.W., Hiemstra P.S., van Dissel J.T.Ann. Hematol. 90:151-158(2011) Four novel ELANE mutations in patients with congenital neutropenia.Kurnikova M., Maschan M., Dinova E., Shagina I., Finogenova N., Mamedova E., Polovtseva T., Shagin D., Shcherbina A.Pediatr. Blood Cancer 57:332-335(2011) The spectrum of ELANE mutations and their implications in severe congenital and cyclic neutropenia.Germeshausen M., Deerberg S., Peter Y., Reimer C., Kratz C.P., Ballmaier M.Hum. Mutat. 34:905-914(2013)
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 Research Articles on ELANE    1. Neutrophil elastase was increased in the saliva from COPD patients and additionally distinguished smoking status.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ELANE recombinant proteinPathways associated with ELANE recombinant protein
 Reference Product  PubMed Publications
 CSF3 recombinant protein  >2 publications with ELANE and CSF3
 SLPI recombinant protein  >2 publications with ELANE and SLPI
 CEBPA recombinant protein  >1 publications with ELANE and CEBPA
 MMP2 recombinant protein  >1 publications with ELANE and MMP2
 MMP8 recombinant protein  >1 publications with ELANE and MMP8
 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Amb2 Integrin Signaling Pathway antibodies  Amb2 Integrin Signaling Pathway Diagram
Diseases associated with ELANE recombinant proteinOrgans/Tissues associated with ELANE recombinant protein
 Disease Name  Pubmed Publications
 Neutropenia Antibodies  >52 publications with ELANE and Neutropenia
 Cyclic neutropenia Antibodies  >12 publications with ELANE and Cyclic neutropenia
 Inflammation Antibodies  >7 publications with ELANE and Inflammation
 Neutropenia, Severe Congenital, Autosomal Dominant 1 Antibodies  >5 publications with ELANE and Neutropenia, Severe Congenital, Autosomal Dominant 1
 Peritonitis Antibodies  >2 publications with ELANE and Peritonitis
 Disease Models, Animal Antibodies  >2 publications with ELANE and Disease Models, Animal
 Weight Loss Antibodies  >2 publications with ELANE and Weight Loss
 Chromosome Aberrations Antibodies  >1 publications with ELANE and Chromosome Aberrations
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >37 publications with ELANE and Blood
 Bone Marrow Antibodies  >11 publications with ELANE and Bone Marrow
 Lung Antibodies  >7 publications with ELANE and Lung
 Connective Tissue Antibodies  >2 publications with ELANE and Connective Tissue
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