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Nid1 recombinant protein :: Nidogen-1 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS954331
SDS-Page
Unit / Price
0.01 mg (E-Coli)  /  $110 +1 FREE 8GB USB
0.01 mg (Yeast)  /  $110 +1 FREE 8GB USB
0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $190 +1 FREE 8GB USB
0.1 mg (E-Coli)  /  $285 +1 FREE 8GB USB
0.1 mg (Yeast)  /  $285 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $460 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $750 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $930 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,170 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,170 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
1 mg (Yeast)  /  $1,180 +1 FREE 8GB USB
0.5 mg (Baculovirus)  /  $1,690 +2 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $1,775 +2 FREE 8GB USB
1 mg (Baculovirus)  /  $2,255 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Nidogen-1 (Nid1), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Mouse Nidogen-1

 Product Synonym Names    Entactin
 Product Gene Name   

Nid1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 MBS954331 COA    COA PDF
 Sequence Positions    428-665aa; Partial
 Sequence    SPQRVNGKVK GRIFVGSSQV PVVFENTDLH SYVVMNHGRS YTAISTIPET VGYSLLPLAP IGGIIGWMFA VEQDGFKNGF SITGGEFTRQ AEVTFLGHPG KLVLKQQFSG IDEHGHLTIS TELEGRVPQI PYGASVHIEP YTELYHYSSS VITSSSTREY TVMEPDQDGA APSHTHIYQW RQTITFQECA HDDARPALPS TQQLSVDSVF VLYNKEERIL RYALSNSIGP VRDGSPDA
 3D Structure    ModBase 3D Structure for P10493
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Production Note    Special Offer: The yeast host-expressed protein is manufactured from a stock plasmid containing the protein gene. Yeast host-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The yeast host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select yeast host-expressed protein for the fastest delivery among all hosts. Please contact us or email to support@mybiosource.com for more details.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of Nid1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Nid1 recombinant protein

   Sulfated glycoprotein widely distributed in basent membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
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 SDS-Page of Nid1 recombinant protein    Nid1 recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for Nid1. It may not necessarily be applicable to this product.
 NCBI GI #    171543883
 NCBI GeneID    18073
 NCBI Accession #    NP_035047.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_010917.2 [Other Products]
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 UniProt Primary Accession #    P10493 [Other Products]
 UniProt Secondary Accession #    Q3TKX9; Q8BQI3; Q8C3U8; Q8C9P6 [Other Products]
 UniProt Related Accession #    P10493 [Other Products]
 Molecular Weight    30.3kD
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 NCBI Official Full Name    nidogen-1
 NCBI Official Synonym Full Names    nidogen 1
 NCBI Official Symbol    Nid1 [Similar Products]
 NCBI Official Synonym Symbols   
Nid; entactin; nidogen-1; A630025O17; entactin-1
[Similar Products]
 NCBI Protein Information    nidogen-1
 UniProt Protein Name    Nidogen-1
 UniProt Synonym Protein Names   
Entactin
 Protein Family    Nidogen
 UniProt Gene Name    Nid1 [Similar Products]
 UniProt Synonym Gene Names    Ent; NID-1 [Similar Products]
 UniProt Entry Name    NID1_MOUSE
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 UniProt Comments for Nid1    Nidogen-1: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: basal lamina; basement membrane; extracellular matrix; extracellular region; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; collagen binding; extracellular matrix binding; laminin binding; laminin-1 binding; protein binding; proteoglycan binding

Biological Process: cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; glomerular basement membrane development
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Product References and Citations for Nid1 recombinant protein

   Amino acid sequence and domain structure of entactin. Homology with epidermal growth factor precursor and low density lipoprotein receptor.Durkin M.E., Chakravarti S., Bartos B.B., Liu S.H., Friedman R.L., Chung A.E.J. Cell Biol. 107:2749-2756(1988) Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells.Mann K., Deutzmann R., Aumailley M., Timpl R., Raimondi L., Yamamda Y., Pan T.-C., Conway D., Chu M.-L.EMBO J. 8:65-72(1989) Characterization of the 5' end of the mouse Ent gene encoding the basement membrane protein, entactin.Durkin M.E., Liu S.H., Reing J., Chung A.E.Gene 132:261-266(1993) Exon organization of the mouse entactin gene corresponds to the structural domains of the polypeptide and has regional homology to the low-density lipoprotein receptor gene.Durkin M.E., Wewer U.M., Chung A.E.Genomics 26:219-228(1995) Purification and structural characterization of intact and fragmented nidogen obtained from a tumor basement membrane.Paulsson M., Deutzmann R., Dziadek M., Nowack H., Timpl R., Weber S., Engel J.Eur. J. Biochem. 156:467-478(1986) Structure and localization of O- and N-linked oligosaccharide chains on basement membrane protein nidogen.Fujiwara S., Shinkai H., Mann K., Timpl R.Matrix 13:215-222(1993) Binding of fibulin-1 to nidogen depends on its C-terminal globular domain and a specific array of calcium-binding epidermal growth factor-like (EG) modules.Adam S., Goehring W., Wiedemann H., Chu M.-L., Timpl R., Kostka G.J. Mol. Biol. 272:226-236(1997) Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin.Sasaki T., Brakebusch C., Engel J., Timpl R.EMBO J. 17:1606-1613(1998) Recombinant domains of mouse nidogen-1 and their binding to basement membrane proteins and monoclonal antibodies.Ries A., Goehring W., Fox J.W., Timpl R., Sasaki T.Eur. J. Biochem. 268:5119-5128(2001) Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo.Lee H.K., Seo I.A., Park H.K., Park H.T.FEBS Lett. 580:2253-2257(2006) Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1.Hopf M., Gohring W., Ries A., Timpl R., Hohenester E.Nat. Struct. Biol. 8:634-640(2001)
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 Research Articles on Nid1    1. These studies provide strong evidence for a pathophysiological link between meprin beta and urinary excretion of cleaved nidogen-1 during cisplatin-induced acute kidney injury.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Nid1 recombinant proteinPathways associated with Nid1 recombinant protein
 Reference Product  PubMed Publications
 Nid2 recombinant protein  >6 publications with Nid1 and Nid2
 Mmp14 recombinant protein  >1 publications with Nid1 and Mmp14
 Hspg2 recombinant protein  >1 publications with Nid1 and Hspg2
 Lamc1 recombinant protein  >1 publications with Nid1 and Lamc1
 Products by Pathway  Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Laminin Interactions Pathway antibodies  Laminin Interactions Pathway Diagram
 PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies  PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
 XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies  XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram
Diseases associated with Nid1 recombinant proteinOrgans/Tissues associated with Nid1 recombinant protein
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >5 publications with Nid1 and Adenocarcinoma
 Disease Models, Animal Antibodies  >5 publications with Nid1 and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >3 publications with Nid1 and Neoplasm Metastasis
 Necrosis Antibodies  >3 publications with Nid1 and Necrosis
 Inflammation Antibodies  >3 publications with Nid1 and Inflammation
 Ovarian Neoplasms Antibodies  >2 publications with Nid1 and Ovarian Neoplasms
 Fibrosis Antibodies  >2 publications with Nid1 and Fibrosis
 Brain Diseases Antibodies  >2 publications with Nid1 and Brain Diseases
 Nervous System Diseases Antibodies  >2 publications with Nid1 and Nervous System Diseases
 Lung Neoplasms Antibodies  >1 publications with Nid1 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >3 publications with Nid1 and Eye
 Brain Antibodies  >3 publications with Nid1 and Brain
 Ovary Antibodies  >3 publications with Nid1 and Ovary
 Heart Antibodies  >2 publications with Nid1 and Heart
 Lung Antibodies  >2 publications with Nid1 and Lung
 Kidney Antibodies  >2 publications with Nid1 and Kidney
 Other Antibodies  >2 publications with Nid1 and Other
 Muscle Antibodies  >1 publications with Nid1 and Muscle
 Spleen Antibodies  >1 publications with Nid1 and Spleen
 Skin Antibodies  >1 publications with Nid1 and Skin
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