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NCL recombinant protein :: Nucleolin (NCL) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2010481 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
SDS-PAGE
Unit / Price
0.01 mg  /  $145 +1 FREE 8GB USB
0.05 mg  /  $280 +1 FREE 8GB USB
0.1 mg  /  $440 +1 FREE 8GB USB
0.2 mg  /  $540 +1 FREE 8GB USB
0.5 mg  /  $1,040 +1 FREE 8GB USB
1 mg  /  $1,545 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Nucleolin (NCL), Recombinant Protein

 Also Known As   

Recombinant Nucleolin (NCL)

 Product Gene Name   

NCL recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF- YVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE GRTIRLELQG SNSRSQPS
 Chromosome Location    Chromosome: 1; NC_000067.6 (86344719..86359455, complement). Location: 1 D; 1 43.94 cM
 3D Structure    ModBase 3D Structure for P09405
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    Tyr353~Ser568 (Accession # P09405) with N-terminal His-Tag
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of NCL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for NCL recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for NCL recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of NCL recombinant protein    NCL recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for NCL. It may not necessarily be applicable to this product.
 NCBI GI #    84875537
 NCBI GeneID    17975
 NCBI Accession #    NP_035010.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_010880.3 [Other Products]
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 UniProt Primary Accession #    P09405 [Other Products]
 UniProt Secondary Accession #    Q548M9; Q61991; Q8BQD8; Q99K50 [Other Products]
 UniProt Related Accession #    P09405 [Other Products]
 Molecular Weight    25.8kDa
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 NCBI Official Full Name    nucleolin
 NCBI Official Synonym Full Names    nucleolin
 NCBI Official Symbol    Ncl [Similar Products]
 NCBI Official Synonym Symbols   
C23; Nucl; D0Nds28; D1Nds28; B530004O11Rik
[Similar Products]
 NCBI Protein Information    nucleolin
 UniProt Protein Name    Nucleolin
 UniProt Synonym Protein Names   
Protein C23
 Protein Family    Nucleolin
 UniProt Gene Name    Ncl [Similar Products]
 UniProt Synonym Gene Names    Nuc [Similar Products]
 UniProt Entry Name    NUCL_MOUSE
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 UniProt Comments for NCL    NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.

Protein type: Helicase; RNA-binding; DNA-binding; Nuclear receptor co-regulator; Nucleolus

Cellular Component: nucleoplasm; cell surface; dense fibrillar component; membrane; cytoplasm; fibrillar center; nucleolus; cell cortex; nucleus; ribonucleoprotein complex

Molecular Function: protein C-terminus binding; identical protein binding; histone binding; RNA binding; nucleotide binding; laminin binding; telomeric DNA binding; calcium ion binding; protein binding; nucleic acid binding; rRNA primary transcript binding; DNA binding; sequence-specific DNA binding; single-stranded DNA binding; receptor binding

Biological Process: endocytosis; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of tumor necrosis factor production; negative regulation of apoptosis
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 Research Articles on NCL    1. Data indicate that increased expression of interleukin-9 receptor (Il9R) is associated with an increased binding of nucleolin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with NCL recombinant proteinDiseases associated with NCL recombinant protein
 Products by Pathway  Pathway Diagram
 PluriNetWork Pathway antibodies  PluriNetWork Pathway Diagram
 T Cell Receptor Signaling Pathway antibodies  T Cell Receptor Signaling Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1205 publications with NCL and Nervous System Diseases
 Brain Diseases Antibodies  >609 publications with NCL and Brain Diseases
 Neoplasms Antibodies  >567 publications with NCL and Neoplasms
 Cardiovascular Diseases Antibodies  >358 publications with NCL and Cardiovascular Diseases
 Heart Diseases Antibodies  >155 publications with NCL and Heart Diseases
 Disease Models, Animal Antibodies  >143 publications with NCL and Disease Models, Animal
 Liver Diseases Antibodies  >136 publications with NCL and Liver Diseases
 Atrophy Antibodies  >123 publications with NCL and Atrophy
 Pain Antibodies  >119 publications with NCL and Pain
 Seizures Antibodies  >98 publications with NCL and Seizures
Organs/Tissues associated with NCL recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >771 publications with NCL and Brain
 Eye Antibodies  >125 publications with NCL and Eye
 Intestine Antibodies  >64 publications with NCL and Intestine
 Embryonic Tissue Antibodies  >37 publications with NCL and Embryonic Tissue
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