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OCLN recombinant protein :: Occludin (OCLN) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2012329 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment) OCLN recombinant protein
Unit / Price
0.01 mg  /  $185 +1 FREE 8GB USB
0.05 mg  /  $380 +1 FREE 8GB USB
0.1 mg  /  $585 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Occludin (OCLN), Recombinant Protein

 Also Known As   

Recombinant Occludin (OCLN)

 Product Gene Name   

OCLN recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-FKPN HYAPSNDMYG GEMHVRPMLS QPAYSFYPED EILHFYKWTS PPGVIRILSM LIIVMCIAIF ACVASTLAWD RGYGTGLFGG SLNYPYS
 Chromosome Location    Chromosome: 13; NC_000079.6 (100496504..100553692, complement). Location: 13 D1; 13 53.23 cM
 3D Structure    ModBase 3D Structure for Q61146
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    Phe17~Ser107 (Accession # Q61146) with N-terminal His-Tag
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of OCLN recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for OCLN recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for OCLN recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product.
 NCBI GI #    6679162
 NCBI GeneID    18260
 NCBI Accession #    NP_032782.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008756.2 [Other Products]
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 UniProt Primary Accession #    Q61146 [Other Products]
 UniProt Secondary Accession #    Q544A7 [Other Products]
 UniProt Related Accession #    Q61146 [Other Products]
 Molecular Weight    11.8kDa
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 NCBI Official Full Name    occludin
 NCBI Official Synonym Full Names    occludin
 NCBI Official Symbol    Ocln [Similar Products]
 NCBI Official Synonym Symbols   
Ocl; AI503564
[Similar Products]
 NCBI Protein Information    occludin
 UniProt Protein Name    Occludin
 Protein Family    Occludin
 UniProt Gene Name    Ocln [Similar Products]
 UniProt Synonym Gene Names    Ocl [Similar Products]
 UniProt Entry Name    OCLN_MOUSE
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 UniProt Comments for OCLN    occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: tight junction; cell surface; membrane; endocytic vesicle; apicolateral plasma membrane; apical plasma membrane; plasma membrane; integral to membrane; intercellular junction; cytoplasmic vesicle; cell junction; cytosol

Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; S-adenosylmethionine-dependent methyltransferase activity

Biological Process: methylation; intercellular junction assembly and maintenance; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process
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 Research Articles on OCLN    1. actin cytoskeletal dynamics is detrimental to METH-induced BBB dysfunction by increasing internalization of occludin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with OCLN recombinant proteinDiseases associated with OCLN recombinant protein
 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 PluriNetWork Pathway antibodies  PluriNetWork Pathway Diagram
 PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies  PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >136 publications with OCLN and Inflammation
 Disease Models, Animal Antibodies  >109 publications with OCLN and Disease Models, Animal
 Neoplasms Antibodies  >100 publications with OCLN and Neoplasms
 Necrosis Antibodies  >78 publications with OCLN and Necrosis
 Cardiovascular Diseases Antibodies  >74 publications with OCLN and Cardiovascular Diseases
 Edema Antibodies  >58 publications with OCLN and Edema
 Hepatitis Antibodies  >38 publications with OCLN and Hepatitis
 Liver Diseases Antibodies  >38 publications with OCLN and Liver Diseases
 Retinal Diseases Antibodies  >32 publications with OCLN and Retinal Diseases
 Colitis Antibodies  >27 publications with OCLN and Colitis
Organs/Tissues associated with OCLN recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >260 publications with OCLN and Brain
 Intestine Antibodies  >213 publications with OCLN and Intestine
 Liver Antibodies  >114 publications with OCLN and Liver
 Eye Antibodies  >97 publications with OCLN and Eye
 Kidney Antibodies  >66 publications with OCLN and Kidney
 Testis Antibodies  >55 publications with OCLN and Testis
 Embryonic Tissue Antibodies  >50 publications with OCLN and Embryonic Tissue
 Lung Antibodies  >44 publications with OCLN and Lung
 Skin Antibodies  >38 publications with OCLN and Skin
 Stomach Antibodies  >18 publications with OCLN and Stomach
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