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PAI-1 recombinant protein

Scan QR to view Datasheet Catalog #    MBS203116
SDS-PAGE
Unit / Price
0.1 mg  /  $310 +1 FREE 8GB USB
0.5 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PAI-1, Recombinant Protein

 Also Known As   

PAI-1, 24-402aa, Human, His-tag, Recombinant, E Coli

 Product Synonym Names    PLANH1; SERPINE1; PAI1; PLANH1; Plasminogen activator inhibitor-1; Plasminogen activator inhibitor-1
 Product Gene Name   

PAI-1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP
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 Purity/Purification    > 95% by SDS - PAGE
 Form/Format    In 150mM Na2HPO4, pH 6.6, 0.5mM NaCl, 2mM Glutathione, 0.01% Tween-80
 Concentration    1 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag    His-tag
 Expression System    E Coli
 Bioactivity    Specific activity, the IC50 value is < 56 nM. Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.
 Assay    1. Prepare a recombinant PAI-1 with various concentrations (2,000 nM, 1,000 nM, 500 nM, 250 nM, 125 nM) in assay buffer. (Assay buffer: 50 mM Tris-HCl pH 8.0, 0.01% Tween 20)
2. Mix equal volumes of the PAI-1 protein and diluted 2 ug/ml uPA (Prospec. Cat No. ENZ-264)
3. Incubate at room temperature for 15 minutes.
4. After dilute incubated reaction mixtures (PAI-1, uPA) by a 5 fold dilution in assay buffer, mix 50ul of 200uM substrate (Z-GGR-AMC) and diluted mixtures.
4. Read excitation wavelength 355nm and emission wavelength 460nm for 10 minutes.
- Z-GGR-AMC (CALBIOCHEM, Cat.No, 672159)
- 96 well Polystyrene Microplate, black (greniner bio-one, Cat.No, 655077)
- Fluorescent plate reader (PerkinElmer, VICTORX3)
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of PAI-1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for PAI-1 recombinant protein

   Plasminogen activator inhibitor-1(PAI-1), a member of the serine protease inhibitor(serpin) super family. PAI-1 is the principal inhibitor of tissue plasminogen activator and urokinase, the activators of plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium but is also secreted by other tissue types, such as adipose tissue. Recombinant human PAI-1, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
 Product Categories/Family for PAI-1 recombinant protein    Enzymes & Proteases
 Applications Tested/Suitable for PAI-1 recombinant protein   

SDS-PAGE

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 SDS-PAGE of PAI-1 recombinant protein    PAI-1 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for PAI-1. It may not necessarily be applicable to this product.
 NCBI GI #    10835159
 NCBI GeneID    5054
 NCBI Accession #    NP_000593 [Other Products]
 NCBI GenBank Nucleotide #    NM_000602.4 [Other Products]
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 UniProt Secondary Accession #    B7Z4S0; F8WD53 [Other Products]
 UniProt Related Accession #    P05121 [Other Products]
 Molecular Weight    45 kDa (400 aa)
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 NCBI Official Full Name    plasminogen activator inhibitor 1
 NCBI Official Synonym Full Names    serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
 NCBI Official Symbol    SERPINE1 [Similar Products]
 NCBI Official Synonym Symbols   
PAI; PAI1; PAI-1; PLANH1
[Similar Products]
 NCBI Protein Information    plasminogen activator inhibitor 1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin E1
 UniProt Protein Name    Plasminogen activator inhibitor 1
 UniProt Synonym Protein Names   
Endothelial plasminogen activator inhibitor; Serpin E1
 UniProt Gene Name    SERPINE1 [Similar Products]
 UniProt Synonym Gene Names    PAI1; PLANH1; PAI; PAI-1 [Similar Products]
 UniProt Entry Name    PAI1_HUMAN
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 NCBI Summary for PAI-1    This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
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 UniProt Comments for PAI-1    SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; negative regulation of fibrinolysis; platelet degranulation; transforming growth factor beta receptor signaling pathway; regulation of receptor activity; angiogenesis; chronological cell aging; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; transcription initiation from RNA polymerase II promoter; platelet activation; transcription, DNA-dependent; negative regulation of blood coagulation; regulation of cell proliferation; fibrinolysis; positive regulation of angiogenesis; positive regulation of transcription from RNA polymerase II promoter; gene expression; blood coagulation; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor-1 Deficiency
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Product References and Citations for PAI-1 recombinant protein

   Gorlatova NV., et al.(2007) J Biol Chem. 282(12):9288-96.
Renckens R., et al. (2006) J Immunol. 177(11):8171-6.
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 Research Articles on PAI-1    1. Data suggest plasma PAI1 level can be modulated by changes in diet/physical activity; each 8.3 g/day higher dietary fiber intake is associated with 9.2% lower PAI1 level (at baseline and persisting after weight loss and improved physical fitness).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PAI-1 recombinant proteinPathways associated with PAI-1 recombinant protein
 Reference Product  PubMed Publications
 TGFB1 recombinant protein  >528 publications with PAI-1 and TGFB1
 SMAD4 recombinant protein  >82 publications with PAI-1 and SMAD4
 HIF1A recombinant protein  >66 publications with PAI-1 and HIF1A
 EGF recombinant protein  >63 publications with PAI-1 and EGF
 PLG recombinant protein  >43 publications with PAI-1 and PLG
 PLAT recombinant protein  >24 publications with PAI-1 and PLAT
 PLAU recombinant protein  >13 publications with PAI-1 and PLAU
 VTN recombinant protein  >5 publications with PAI-1 and VTN
 ALB recombinant protein  >3 publications with PAI-1 and ALB
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 BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway antibodies  BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
Diseases associated with PAI-1 recombinant proteinOrgans/Tissues associated with PAI-1 recombinant protein
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3209 publications with PAI-1 and Cardiovascular Diseases
 Thrombosis Antibodies  >1401 publications with PAI-1 and Thrombosis
 Inflammation Antibodies  >1327 publications with PAI-1 and Inflammation
 Heart Diseases Antibodies  >1052 publications with PAI-1 and Heart Diseases
 Necrosis Antibodies  >894 publications with PAI-1 and Necrosis
 Obesity Antibodies  >879 publications with PAI-1 and Obesity
 Atherosclerosis Antibodies  >780 publications with PAI-1 and Atherosclerosis
 Hypertension Antibodies  >702 publications with PAI-1 and Hypertension
 Fibrosis Antibodies  >693 publications with PAI-1 and Fibrosis
 Disease Models, Animal Antibodies  >540 publications with PAI-1 and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5335 publications with PAI-1 and Blood
 Vascular Antibodies  >2836 publications with PAI-1 and Vascular
 Heart Antibodies  >939 publications with PAI-1 and Heart
 Kidney Antibodies  >812 publications with PAI-1 and Kidney
 Muscle Antibodies  >740 publications with PAI-1 and Muscle
 Liver Antibodies  >618 publications with PAI-1 and Liver
 Connective Tissue Antibodies  >588 publications with PAI-1 and Connective Tissue
 Lung Antibodies  >582 publications with PAI-1 and Lung
 Adipose Tissue Antibodies  >524 publications with PAI-1 and Adipose Tissue
 Brain Antibodies  >399 publications with PAI-1 and Brain
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