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PAI-1 recombinant protein

Scan QR to view Datasheet Catalog #    MBS232424 PAI-1 recombinant protein
Unit / Price
0.01 mg  /  $440 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PAI-1, Recombinant Protein

 Also Known As   

RECOMBINANT HUMAN PAI-1

 Product Gene Name   

PAI-1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    173360
 3D Structure    ModBase 3D Structure for P05121
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 Purity/Purification    >95% by SDS PAGE and HPLC analysis.
 Form/Format    Purified recombinant protein - lyophilised
 Concentration    Approximate Protein Concentration: 0.1mg/ml after reconstitution (lot specific)
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 Reconstitution    Reconstitute with 100ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended sotrage, the addition of 0.1% BSA is recommended.
Preparation
 Buffer Solution    10mM Sodium Phosphate
 Activity    Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37 degree C. Half maximal inhibition against 1.0 mug/ml of single chain tPA was obtained at a concentration of 2.0 mug/ml.
 Target Species    Human
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 Preparation and Storage    Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
 Other Notes    Small volumes of PAI-1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for PAI-1 recombinant protein

   PAI-1 (plasminogen activator inhibitor-1) is a serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), and hence fibrinolysis. Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and VLDL (very-low-density lipoprotein).
 Applications Tested/Suitable for PAI-1 recombinant protein   

Functional Assays (FN)

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NCBI/Uniprot data below describe general gene information for PAI-1. It may not necessarily be applicable to this product.
 NCBI GI #    10835159
 NCBI GeneID    5054
 NCBI Accession #    NP_000593.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000602.4 [Other Products]
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 UniProt Primary Accession #    P05121 [Other Products]
 UniProt Secondary Accession #    B7Z4S0; F8WD53 [Other Products]
 UniProt Related Accession #    P05121 [Other Products]
 Molecular Weight    42.7kD (379 amino acid residues).
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 NCBI Official Full Name    plasminogen activator inhibitor 1
 NCBI Official Synonym Full Names    serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
 NCBI Official Symbol    SERPINE1 [Similar Products]
 NCBI Official Synonym Symbols   
PAI; PAI1; PAI-1; PLANH1
[Similar Products]
 NCBI Protein Information    plasminogen activator inhibitor 1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin E1
 UniProt Protein Name    Plasminogen activator inhibitor 1
 UniProt Synonym Protein Names   
Endothelial plasminogen activator inhibitor; Serpin E1
 UniProt Gene Name    SERPINE1 [Similar Products]
 UniProt Synonym Gene Names    PAI1; PLANH1; PAI; PAI-1 [Similar Products]
 UniProt Entry Name    PAI1_HUMAN
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 NCBI Summary for PAI-1    This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
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 UniProt Comments for PAI-1    SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; transcription initiation from RNA polymerase II promoter; platelet activation; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; transcription, DNA-dependent; negative regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; regulation of cell proliferation; positive regulation of interleukin-8 production; positive regulation of angiogenesis; fibrinolysis; negative regulation of fibrinolysis; platelet degranulation; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; angiogenesis; regulation of receptor activity; blood coagulation; chronological cell aging; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor-1 Deficiency
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 Research Articles on PAI-1    1. The carriers of polymorphism 4G are at greater risk for early pregnancy loss than those with 5G, which confirms that carriage of the polymorphism 4G/5G 675 gene PAI-1 has a specific in pathogenesis and expression of Polycystic Ovary Syndrome.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PAI-1 recombinant proteinPathways associated with PAI-1 recombinant protein
 Reference Product  PubMed Publications
 TGFB1 recombinant protein  >531 publications with PAI-1 and TGFB1
 SMAD4 recombinant protein  >82 publications with PAI-1 and SMAD4
 HIF1A recombinant protein  >66 publications with PAI-1 and HIF1A
 EGF recombinant protein  >63 publications with PAI-1 and EGF
 PLG recombinant protein  >43 publications with PAI-1 and PLG
 PLAT recombinant protein  >24 publications with PAI-1 and PLAT
 PLAU recombinant protein  >13 publications with PAI-1 and PLAU
 VTN recombinant protein  >5 publications with PAI-1 and VTN
 ALB recombinant protein  >3 publications with PAI-1 and ALB
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway antibodies  BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
Diseases associated with PAI-1 recombinant proteinOrgans/Tissues associated with PAI-1 recombinant protein
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3215 publications with PAI-1 and Cardiovascular Diseases
 Thrombosis Antibodies  >1406 publications with PAI-1 and Thrombosis
 Inflammation Antibodies  >1333 publications with PAI-1 and Inflammation
 Heart Diseases Antibodies  >1055 publications with PAI-1 and Heart Diseases
 Necrosis Antibodies  >896 publications with PAI-1 and Necrosis
 Obesity Antibodies  >883 publications with PAI-1 and Obesity
 Atherosclerosis Antibodies  >781 publications with PAI-1 and Atherosclerosis
 Hypertension Antibodies  >703 publications with PAI-1 and Hypertension
 Fibrosis Antibodies  >695 publications with PAI-1 and Fibrosis
 Disease Models, Animal Antibodies  >542 publications with PAI-1 and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5356 publications with PAI-1 and Blood
 Vascular Antibodies  >2842 publications with PAI-1 and Vascular
 Heart Antibodies  >940 publications with PAI-1 and Heart
 Kidney Antibodies  >813 publications with PAI-1 and Kidney
 Muscle Antibodies  >741 publications with PAI-1 and Muscle
 Liver Antibodies  >620 publications with PAI-1 and Liver
 Connective Tissue Antibodies  >589 publications with PAI-1 and Connective Tissue
 Lung Antibodies  >584 publications with PAI-1 and Lung
 Adipose Tissue Antibodies  >525 publications with PAI-1 and Adipose Tissue
 Brain Antibodies  >401 publications with PAI-1 and Brain
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