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PDE1A recombinant protein

Scan QR to view Datasheet Catalog #    MBS515627
Unit / Price
0.005 mg  /  $315 +1 FREE 8GB USB
0.01 mg  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PDE1A, Recombinant Protein

★Popular Item★
 Also Known As   

PDE1A, Active

 Product Gene Name   

PDE1A recombinant protein

[Similar Products]
 Product Synonym Gene Name    HCAM1; HSPDE1A; MGC26303 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    Full Length
 OMIM    U40370 mRNA
 3D Structure    ModBase 3D Structure for P54750
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 Specificity    Full length protein expressed in Sf9 cells
 Purity/Purification    >80%
 Form/Format    Recombinant protein stored in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
 Concentration    0.1 ug/ml (lot specific)
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 Preparation and Storage    Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Ships with dry ice.
 Other Notes    Small volumes of PDE1A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for PDE1A recombinant protein

   Description: Recombinant full-length human PDE1A was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Scientific Background: PDE1A is a member of the cyclic nucleotide phosphodiesterase family that is stimulated by Ca2+-calmodulin. PDE1A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5'-GMP. Calmodulin is tightly bound to PDE1A in human spermatozoa and this results in permanent activation of PDE1A (1). Inhibition of PDE1A function significantly attenuates vascular smooth muscle cell growth by decreasing proliferation via G1 arrest and induces apoptosis (2). In vascular smooth muscle cell, PDE1A is important in regulating growth and survival of these cells and thus can contribute to the neointima formation in atherosclerosis and restenosis.
 Product Categories/Family for PDE1A recombinant protein    Enzymes; Active Phosphodiesterases
 Applications Tested/Suitable for PDE1A recombinant protein   

PDE Assay

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 Testing Data of PDE1A recombinant protein    The specific activity of PDE1A was determined to be 10,500 nmol/min/mg as per activity assay protocol
PDE1A recombinant protein Testing Data image
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 Testing Data of PDE1A recombinant protein    The purity was determined to be >80% by densitometry. Approx. MW 64kDa.
PDE1A recombinant protein Testing Data image
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NCBI/Uniprot data below describe general gene information for PDE1A. It may not necessarily be applicable to this product.
 NCBI GI #    385198066
 NCBI GeneID    5136
 NCBI Accession #    NM_005019.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_005019 [Other Products]
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 UniProt Primary Accession #    P54750 [Other Products]
 UniProt Secondary Accession #    Q86VZ0; Q9C0K8; Q9C0K9; Q9C0L0; Q9C0L1; Q9C0L2; Q9C0L3; Q9C0L4; Q9UFX3; D3DPG5 [Other Products]
 UniProt Related Accession #    P54750 [Other Products]
 Molecular Weight    ~64 kDa
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 NCBI Official Full Name    Homo sapiens phosphodiesterase 1A (PDE1A), transcript variant 1, mRNA
 NCBI Official Synonym Full Names    phosphodiesterase 1A
 NCBI Official Symbol    PDE1A [Similar Products]
 NCBI Official Synonym Symbols   
HCAM1; HCAM-1; HSPDE1A; CAM-PDE-1A
[Similar Products]
 NCBI Protein Information    calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
 UniProt Protein Name    Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
 UniProt Synonym Protein Names   
61 kDa Cam-PDE; hCam-1
 Protein Family    Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase
 UniProt Gene Name    PDE1A [Similar Products]
 UniProt Synonym Gene Names    Cam-PDE 1A [Similar Products]
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 NCBI Summary for PDE1A    Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]
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 UniProt Comments for PDE1A    PDE1A: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-GMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity
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Product References and Citations for PDE1A recombinant protein

   1. Lefišvre L, et al: Presence of cyclic nucleotide phosphodiesterases PDE1A, existing as a stable complex with calmodulin, and PDE3A in human spermatozoa. Biol Reprod. 2002 Aug;67 (2):423-30. 2. Nagel D J, et al: Role of nuclear Ca2+/calmodulin-stimulated phosphodiesterase 1A in vascular smooth muscle cell growth and survival. Circ Res. 2006 Mar 31;98 (6):777-84.
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 Research Articles on PDE1A    1. Report significant associations of PDE1A single nucleotide polymorphisms with diastolic blood pressure and carotid intima-media thickness.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PDE1A recombinant proteinDiseases associated with PDE1A recombinant protein
 Products by Pathway  Pathway Diagram
 Ca-dependent Events Pathway antibodies  Ca-dependent Events Pathway Diagram
 CaM Pathway antibodies  CaM Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calmodulin Induced Events Pathway antibodies  Calmodulin Induced Events Pathway Diagram
 Cam-PDE 1 Activation Pathway antibodies  Cam-PDE 1 Activation Pathway Diagram
 DAG And IP3 Signaling Pathway antibodies  DAG And IP3 Signaling Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >16 publications with PDE1A and Cardiovascular Diseases
 Hypertension Antibodies  >14 publications with PDE1A and Hypertension
 Hypertrophy Antibodies  >6 publications with PDE1A and Hypertrophy
 Cardiomegaly Antibodies  >5 publications with PDE1A and Cardiomegaly
 Inflammation Antibodies  >4 publications with PDE1A and Inflammation
 Disease Models, Animal Antibodies  >3 publications with PDE1A and Disease Models, Animal
 Fibrosis Antibodies  >2 publications with PDE1A and Fibrosis
 Kidney Diseases Antibodies  >2 publications with PDE1A and Kidney Diseases
 Immune System Diseases Antibodies  >2 publications with PDE1A and Immune System Diseases
 Neurobehavioral Manifestations Antibodies  >2 publications with PDE1A and Neurobehavioral Manifestations
Organs/Tissues associated with PDE1A recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >30 publications with PDE1A and Muscle
 Brain Antibodies  >13 publications with PDE1A and Brain
 Lung Antibodies  >11 publications with PDE1A and Lung
 Kidney Antibodies  >7 publications with PDE1A and Kidney
 Thyroid Antibodies  >4 publications with PDE1A and Thyroid
 Testis Antibodies  >4 publications with PDE1A and Testis
 Eye Antibodies  >3 publications with PDE1A and Eye
 Embryonic Tissue Antibodies  >2 publications with PDE1A and Embryonic Tissue
 Ganglia Antibodies  >2 publications with PDE1A and Ganglia
 Trachea Antibodies  >1 publications with PDE1A and Trachea
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