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PYCR1 recombinant protein :: PYCR1, aa1-319 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS636973 PYCR1 recombinant protein
Unit / Price
0.05 mg  /  $570 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PYCR1, aa1-319, Recombinant Protein

 Also Known As   

PYCR1, aa1-319, Recombinant, Human (Pyrroline-5-carboxylate Reductase 1, P5C Reductase 1, P5CR 1, ARCL2B, P5C, P5CR, PIG45, PP222, PRO3, PYCR)

 Product Gene Name   

PYCR1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDLA TVSALRKMGV KLTPHNKETV QHSDVLFLAV KPHIIPFILD EIGADIEDRH IVVSCAAGVT ISSIEKKLSA FRPAPRVIRC MTNTPVVVRE GATVYATGTH AQVEDGRLME QLLSSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR LAVRLGAQAL LGAAKMLLHS EQHPGQLKDN VSSPGGATIH ALHVLESGGF RSLLINAVEA SCIRTRELQS MADQEQVSPA AIKKTILDKV KLDSPAGTAL SPSGHTKLLP RSLAPAGKD
 Chromosome Location    Chromosome: 17; NC_000017.10 (79890260..79895203, complement). Location: 17q25.3
 OMIM    179035
 3D Structure    ModBase 3D Structure for P32322
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 Host    E Coli
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 Purity/Purification    Highly Purified
~90% (SDS-PAGE)
 Form/Format    Supplied as a liquid in 20mM Tris-HCl Buffer, pH 8.5, 10% glycerol.
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 Specific Activity    ~0.03unit/mg
 Biological Activity    One unit will oxidize 1.0umole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25ºC.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of PYCR1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for PYCR1 recombinant protein

   PYCR1 is a universal housekeeping enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B).

Recombinant protein corresponding to human PYCR1, fused to His-tag at N-terminus, expressed in E. coli.
 Product Categories/Family for PYCR1 recombinant protein    Molecular Biology; MB-Enzymes, Reductase
NCBI/Uniprot data below describe general gene information for PYCR1. It may not necessarily be applicable to this product.
 NCBI GI #    533112488
 NCBI GeneID    5831
 NCBI Accession #    NP_001269209.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001282280.1 [Other Products]
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 UniProt Primary Accession #    P32322 [Other Products]
 UniProt Secondary Accession #    Q6FHI4; Q96DI6; Q9HBQ4; A6NFM2 [Other Products]
 UniProt Related Accession #    P32322 [Other Products]
 Molecular Weight    ~35.5kD [Similar Products]
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 NCBI Official Full Name    pyrroline-5-carboxylate reductase 1, mitochondrial isoform 4
 NCBI Official Synonym Full Names    pyrroline-5-carboxylate reductase 1
 NCBI Official Symbol    PYCR1 [Similar Products]
 NCBI Official Synonym Symbols   
P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
[Similar Products]
 NCBI Protein Information    pyrroline-5-carboxylate reductase 1, mitochondrial; P5C reductase 1; proliferation-inducing protein 45
 UniProt Protein Name    Pyrroline-5-carboxylate reductase 1, mitochondrial
 Protein Family    Pyrroline-5-carboxylate reductase
 UniProt Gene Name    PYCR1 [Similar Products]
 UniProt Synonym Gene Names    P5C reductase 1; P5CR 1 [Similar Products]
 UniProt Entry Name    P5CR1_HUMAN
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 NCBI Summary for PYCR1    This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
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 UniProt Comments for PYCR1    PYCR1: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). A multisystem disorder characterized by the appearance of premature aging, wrinkled and lax skin with reduced elasticity, joint laxity, craniofacial dysmorphic features, intrauterine growth retardation with some degree of postnatal growth deficiency, and developmental delay. Defects in PYCR1 are the cause of cutis laxa, autosomal recessive, type 3B (ARCL3B). ARCL3B is a disorder characterized by an aged appearance with distinctive facial features, sparse hair, ophthalmologic abnormalities, intrauterine growth retardation, and cutis laxa. Belongs to the pyrroline-5-carboxylate reductase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.5.1.2; Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: identical protein binding; protein binding; pyrroline-5-carboxylate reductase activity

Biological Process: regulation of mitochondrial membrane potential; proline biosynthetic process; amino acid biosynthetic process

Disease: Cutis Laxa, Autosomal Recessive, Type Iib; Cutis Laxa, Autosomal Recessive, Type Iiib
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 Research Articles on PYCR1    1. Data suggest that DJ-1 and PYCR1 are on the same pathway of anti-oxidative stress protection of the cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PYCR1 recombinant proteinPathways associated with PYCR1 recombinant protein
 Reference Product  PubMed Publications
 GORAB recombinant protein  >3 publications with PYCR1 and GORAB
 ALDH18A1 recombinant protein  >1 publications with PYCR1 and ALDH18A1
 Products by Pathway  Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Proline Biosynthesis, Glutamate => Proline Pathway antibodies  Proline Biosynthesis, Glutamate => Proline Pathway Diagram
 Proline Biosynthesis, Glutamate => Proline Pathway antibodies  Proline Biosynthesis, Glutamate => Proline Pathway Diagram
 Urea Cycle And Metabolism Of Amino Groups Pathway antibodies  Urea Cycle And Metabolism Of Amino Groups Pathway Diagram
Diseases associated with PYCR1 recombinant proteinOrgans/Tissues associated with PYCR1 recombinant protein
 Disease Name  Pubmed Publications
 Cutis Laxa Antibodies  >14 publications with PYCR1 and Cutis Laxa
 Neoplasms Antibodies  >6 publications with PYCR1 and Neoplasms
 Congenital Abnormalities Antibodies  >5 publications with PYCR1 and Congenital Abnormalities
 Adenocarcinoma Antibodies  >3 publications with PYCR1 and Adenocarcinoma
 Cutis Laxa, Autosomal Recessive, Type IIB Antibodies  >2 publications with PYCR1 and Cutis Laxa, Autosomal Recessive, Type IIB
 Precancerous Conditions Antibodies  >1 publications with PYCR1 and Precancerous Conditions
 Lung Neoplasms Antibodies  >1 publications with PYCR1 and Lung Neoplasms
 Progeria Antibodies  >1 publications with PYCR1 and Progeria
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >10 publications with PYCR1 and Skin
 Bone Antibodies  >3 publications with PYCR1 and Bone
 Prostate Antibodies  >2 publications with PYCR1 and Prostate
 Connective Tissue Antibodies  >1 publications with PYCR1 and Connective Tissue
 Blood Antibodies  >1 publications with PYCR1 and Blood
 Lung Antibodies  >1 publications with PYCR1 and Lung
 Brain Antibodies  >1 publications with PYCR1 and Brain
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