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PTCH1 recombinant protein :: Patched 1 (PTCH1) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2011579 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
SDS-PAGE
Unit / Price
0.01 mg  /  $140 +1 FREE 8GB USB
0.05 mg  /  $265 +1 FREE 8GB USB
0.1 mg  /  $405 +1 FREE 8GB USB
0.2 mg  /  $500 +1 FREE 8GB USB
0.5 mg  /  $970 +1 FREE 8GB USB
1 mg  /  $1,435 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Patched 1 (PTCH1), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Patched 1 (PTCH1)

 Product Gene Name   

PTCH1 recombinant protein

[Similar Products]
 Matching Pairs    Antibody: Patched 1 (PTCH1) (MBS2004836)
Antigen: Patched 1 (PTCH1) (MBS2011579)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- LIITPLDC FWEGAKLQSG TAYLLGKPPL RWTNFDPLEF LEELKKINYQ VDSWEEMLNK AEVGHGYMDR PCLNPADPDC PATAPNKNST KPLDVALVLN GGCQGLSRKY MHWQEELIVG GTVKNATGKL VSAHALQTMF QLMTPKQMYE HFRGYDYVSH INWNEDRAAA ILEAWQRTYV EVVHQSVAPN STQKVLPFTT TTLDDILKSF SD
 Chromosome Location    Chromosome: 13; NC_000079.6 (63508328..63573460, complement). Location: 13 B3; 13 32.8 cM
 3D Structure    ModBase 3D Structure for Q61115
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in 20mM Tris, 500mM NaCI, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    Leu213~Asp422 (Accession # Q61115) with two N-terminal Tags, His-tag and T7-tag
 Usage    Reconstitute in ddH2O
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Predicted Isoelectric Point    6.3
 Subcellular Location    Membrane; Multi-pass membrane protein.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of PTCH1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for PTCH1 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of PTCH1 recombinant protein    PTCH1 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for PTCH1. It may not necessarily be applicable to this product.
 NCBI GI #    6679519
 NCBI GeneID    19206
 NCBI Accession #    NP_032983.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008957.2 [Other Products]
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 UniProt Primary Accession #    Q61115 [Other Products]
 UniProt Related Accession #    Q61115 [Other Products]
 Molecular Weight    27.3kDa
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 NCBI Official Full Name    protein patched homolog 1
 NCBI Official Synonym Full Names    patched homolog 1
 NCBI Official Symbol    Ptch1 [Similar Products]
 NCBI Official Synonym Symbols   
Ptc; mes; Ptc1; Ptch; A230106A15Rik
[Similar Products]
 NCBI Protein Information    protein patched homolog 1; Patched 1; Ptch1 protein +12b; mesenchymal dysplasia
 UniProt Protein Name    Protein patched homolog 1
 UniProt Gene Name    Ptch1 [Similar Products]
 UniProt Synonym Gene Names    Ptch; PTC; PTC1 [Similar Products]
 UniProt Entry Name    PTC1_MOUSE
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 UniProt Comments for PTCH1    PTCH1: a multi-pass membrane protein member of the ?patched? family that acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog protein?s signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. Interacts with SNX17. Expressed in tumor cells but not in normal skin. In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH1 are the cause of basal cell nevus syndrome (BCNS), also known as Gorlin syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas, fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 defects is also the cause of holoprosencephaly, the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres.

Protein type: Tumor suppressor; Membrane protein, multi-pass; Membrane protein, integral; Cell cycle regulation

Cellular Component: Golgi apparatus; membrane; intracellular membrane-bound organelle; integral to plasma membrane; perinuclear region of cytoplasm; postsynaptic density; extracellular region; integral to membrane; plasma membrane; caveola; midbody

Molecular Function: heparin binding; protein binding; cyclin binding; zinc ion binding; hedgehog receptor activity; cholesterol binding; protein complex binding; patched binding; smoothened binding

Biological Process: heart morphogenesis; hindlimb morphogenesis; epidermis development; regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; cell fate determination; glucose homeostasis; signal transduction; response to chlorate; negative regulation of cell proliferation; regulation of protein localization; mammary gland development; regulation of growth; negative regulation of osteoblast differentiation; dorsoventral neural tube patterning; negative regulation of epithelial cell proliferation; embryonic limb morphogenesis; smoothened signaling pathway; epidermal cell fate specification; negative regulation of multicellular organism growth; pharyngeal system development; in utero embryonic development; negative regulation of transcription factor activity; neural tube patterning; negative regulation of cell division; keratinocyte proliferation; spinal cord motor neuron differentiation; limb morphogenesis; pattern specification process; regulation of cell proliferation; organ morphogenesis; dorsal/ventral pattern formation; ureteric bud branching; neural plate pattern formation; negative regulation of smoothened signaling pathway; positive regulation of epidermal cell differentiation; neural tube closure; embryonic organ development; protein processing; brain development; neural tube formation; regulation of smoothened signaling pathway
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 Research Articles on PTCH1    1. Hh receptor Ptch is dispensable for T cell function in vitro as well as in vivo
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PTCH1 recombinant proteinDiseases associated with PTCH1 recombinant protein
 Products by Pathway  Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >257 publications with PTCH1 and Neoplasms
 Carcinoma, Basal Cell Antibodies  >147 publications with PTCH1 and Carcinoma, Basal Cell
 Medulloblastoma Antibodies  >67 publications with PTCH1 and Medulloblastoma
 Basal Cell Nevus Syndrome Antibodies  >64 publications with PTCH1 and Basal Cell Nevus Syndrome
 Skin Neoplasms Antibodies  >63 publications with PTCH1 and Skin Neoplasms
 Neuroectodermal Tumors, Primitive Antibodies  >43 publications with PTCH1 and Neuroectodermal Tumors, Primitive
 Brain Neoplasms Antibodies  >36 publications with PTCH1 and Brain Neoplasms
 Brain Diseases Antibodies  >36 publications with PTCH1 and Brain Diseases
 Disease Models, Animal Antibodies  >31 publications with PTCH1 and Disease Models, Animal
 Cell Transformation, Neoplastic Antibodies  >23 publications with PTCH1 and Cell Transformation, Neoplastic
Organs/Tissues associated with PTCH1 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >23 publications with PTCH1 and Embryonic Tissue
 Lung Antibodies  >21 publications with PTCH1 and Lung
 Bone Marrow Antibodies  >11 publications with PTCH1 and Bone Marrow
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