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PRDX5 recombinant protein :: Peroxiredoxin 5 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS203340
SDS-PAGE
Unit / Price
0.1 mg  /  $310 +1 FREE 8GB USB
0.5 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Peroxiredoxin 5 (PRDX5), Recombinant Protein

 Also Known As   

Peroxiredoxin 5, 53-214aa, Human, Recombinant, E Coli

 Product Synonym Names    Peroxiredoxin 5; ACR1; AOEB116; B116; PLP; PMP20; PRDX5; PRXV; SBBI10; AOEB166; PRDX 5; Thioredoxin reductase.
 Product Gene Name   

PRDX5 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MAPIKVGDAI PAVEVFEGEP GNKVNLAELF KGKKGVLFGV PGAFTPGCSK THLPGFVEQA EALKAKGVQV VACLSVNDAF VTGEWGRAHK AEGKVRLLAD PTGAFGKETD LLLDDSLVSI FGNRRLKRFS MVVQDGIVKA LNVEPDGTGL TCSLAPNIIS QL
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 Purity/Purification    > 95% by SDS - PAGE
 Form/Format    Liquid. In 20 mM HEPES buffer (pH 7.4).
 Concentration    1 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Expression System    E Coli
 Bioactivity    Specific activity: approximately 117-136 pmole/min/ug. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX5 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute
 Endotoxin    < 1.0 Eu per 1 microgram of protein (determined by LAL method)
 Assay    1. Prepare a 50ul reaction mix into a suitable container :
The final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol.
2. Add 5 ul of recombinant PRDX5 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer.
3. Incubate at 25C for 2 minutes.
4. Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min.
5. Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction.
6. Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN)
7. Record the increase in A475nm.
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of PRDX5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for PRDX5 recombinant protein

   Peroxiredoxin 5, also known as PRDX5, is a member of the peroxiredoxin family of antioxidant enzymes, which reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. This protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. It has been reported that peroxiredoxin 5 is involved in intracellular redox signaling. Recombinant Peroxiredoxin 5 protein was expressed in E.coli and purified by using conventional chromatography techniques.
 Product Categories/Family for PRDX5 recombinant protein    Redox Proteins
 Applications Tested/Suitable for PRDX5 recombinant protein   

SDS-PAGE

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 SDS-PAGE of PRDX5 recombinant protein    PRDX5 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for PRDX5. It may not necessarily be applicable to this product.
 NCBI GI #    6912238
 NCBI GeneID    25824
 NCBI Accession #    NP_036226 [Other Products]
 NCBI GenBank Nucleotide #    NM_012094.4 [Other Products]
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 UniProt Secondary Accession #    Q14CK0; Q6IAF2; Q9UBU5; Q9UJU4; Q9UKX4; A6NC19; A6NG06; B7ZLJ4; B7ZVW3 [Other Products]
 UniProt Related Accession #    P30044 [Other Products]
 Molecular Weight    17.0 kDa (162aa), confirmed by MALDI-TOF. [Similar Products]
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 NCBI Official Full Name    peroxiredoxin-5, mitochondrial isoform a
 NCBI Official Synonym Full Names    peroxiredoxin 5
 NCBI Official Symbol    PRDX5 [Similar Products]
 NCBI Official Synonym Symbols   
PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55
[Similar Products]
 NCBI Protein Information    peroxiredoxin-5, mitochondrial; Alu co-repressor 1; TPx type VI; antioxidant enzyme B166; epididymis secretory protein Li 55; liver tissue 2D-page spot 71B; peroxiredoxin V; peroxisomal antioxidant enzyme; thioredoxin peroxidase PMP20; thioredoxin reductase
 UniProt Protein Name    Peroxiredoxin-5, mitochondrial
 UniProt Synonym Protein Names   
Alu corepressor 1; Antioxidant enzyme B166; AOEB166; Liver tissue 2D-page spot 71B; PLP; Peroxiredoxin V; Prx-V; Peroxisomal antioxidant enzyme; TPx type VI; Thioredoxin peroxidase PMP20; Thioredoxin reductase
 Protein Family    Peroxiredoxin
 UniProt Gene Name    PRDX5 [Similar Products]
 UniProt Synonym Gene Names    ACR1; AOEB166; Prx-V [Similar Products]
 UniProt Entry Name    PRDX5_HUMAN
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 NCBI Summary for PRDX5    This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PRDX5    PRDX5: Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. Belongs to the peroxiredoxin 2 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 1.11.1.15; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: peroxisomal matrix; extracellular space; mitochondrion; intracellular membrane-bound organelle; mitochondrial matrix; perinuclear region of cytoplasm; cytoplasm; peroxisome; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: antioxidant activity; protein dimerization activity; peroxidase activity; peroxiredoxin activity; caspase inhibitor activity; receptor binding

Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; positive regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase III promoter; negative regulation of oxidoreductase activity; response to oxidative stress; negative regulation of caspase activity; inflammatory response; negative regulation of apoptosis
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Product References and Citations for PRDX5 recombinant protein

   Yamashita H., et al. (1999) J Biol Chem. 274(42):29897-904.
Declercg JP., et al. (2001) J Mol Biol. 311(4):751-9.
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 Research Articles on PRDX5    1. PRX5 is either consumed or its production is impaired in severe stroke
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PRDX5 recombinant proteinPathways associated with PRDX5 recombinant protein
 Reference Product  PubMed Publications
 GPX1 recombinant protein  >3 publications with PRDX5 and GPX1
 GPX4 recombinant protein  >3 publications with PRDX5 and GPX4
 GPX2 recombinant protein  >2 publications with PRDX5 and GPX2
 GPX5 recombinant protein  >1 publications with PRDX5 and GPX5
 TXN2 recombinant protein  >1 publications with PRDX5 and TXN2
 TXN recombinant protein  >1 publications with PRDX5 and TXN
 Products by Pathway  Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Detoxification Of Reactive Oxygen Species Pathway antibodies  Detoxification Of Reactive Oxygen Species Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
 Selenium Pathway antibodies  Selenium Pathway Diagram
 Thioredoxin Pathway antibodies  Thioredoxin Pathway Diagram
Diseases associated with PRDX5 recombinant proteinOrgans/Tissues associated with PRDX5 recombinant protein
 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >6 publications with PRDX5 and Immune System Diseases
 Carcinoma Antibodies  >5 publications with PRDX5 and Carcinoma
 Cardiovascular Diseases Antibodies  >4 publications with PRDX5 and Cardiovascular Diseases
 Inflammation Antibodies  >4 publications with PRDX5 and Inflammation
 Skin Diseases Antibodies  >3 publications with PRDX5 and Skin Diseases
 Alopecia Areata Antibodies  >3 publications with PRDX5 and Alopecia Areata
 Nervous System Diseases Antibodies  >2 publications with PRDX5 and Nervous System Diseases
 Necrosis Antibodies  >2 publications with PRDX5 and Necrosis
 Lung Diseases Antibodies  >2 publications with PRDX5 and Lung Diseases
 Liver Diseases Antibodies  >2 publications with PRDX5 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >11 publications with PRDX5 and Liver
 Blood Antibodies  >11 publications with PRDX5 and Blood
 Brain Antibodies  >7 publications with PRDX5 and Brain
 Lung Antibodies  >6 publications with PRDX5 and Lung
 Kidney Antibodies  >5 publications with PRDX5 and Kidney
 Thyroid Antibodies  >5 publications with PRDX5 and Thyroid
 Muscle Antibodies  >4 publications with PRDX5 and Muscle
 Heart Antibodies  >4 publications with PRDX5 and Heart
 Eye Antibodies  >3 publications with PRDX5 and Eye
 Vascular Antibodies  >3 publications with PRDX5 and Vascular
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