Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Recombinant Protein rightarrow PRDX6  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Putative phospholipase B-like 2 (PLBD2) ELISA Kit
 • Vasodilator Stimulated Phosphoprotein (VASP) Antibody
 • KIN Antibody
 • Heat Shock Protein 70 HSP-70 ELISA Kit
 • Glucose Transporter 4 (GLUT4) ELISA Kit
 • Chemokine C-C-Motif Ligand 14 (CCL14) Antibody
 • fibroblast growth factor 8 (androgen-induced) (FGF8) ELISA Kit
 • PECAM-1 Antibody
 • Influenza A Hemagglutinin H9 Antibody
 • semenogelin I (SEMG1) ELISA Kit
 • 7-dehydrocholesterol reductase (Dhcr7) Recombinant Protein
 • collagen, type IX, alpha 3 (COL9A3) ELISA Kit
 • ZO-1 (TJP1) Antibody
 • alpha Lactalbumin (alpha-La) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PRDX6 recombinant protein :: Peroxiredoxin 6 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS203357
SDS-PAGE
Unit / Price
0.1 mg  /  $310 +1 FREE 8GB USB
0.5 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Peroxiredoxin 6 (PRDX6), Recombinant Protein

 Also Known As   

Peroxiredoxin 6, 1-224aa, Human, His-tag, Recombinant, E Coli

 Product Synonym Names    1-Cys; aiPLA2; AOP2; NSGPx; p29; PRX; Peroxiredoxin6; PRDX6; PRDX 6; PRDX-6; AA690119; Prdx6 rs3; 1 Cys; 1 Cys peroxiredoxin; 1 Cys PRX; 1 cysPrx; 24 kDa protein; 9430088D19Rik; Acidic calcium independent phospholipase A2; Antioxidant protein 2; Aop2 rs3; Brp 12; mKIAA0106; Ciliary body glutathione peroxidase; CP 3; EC 1.11.1.15; EC 1.11.1.7; EC 3.1.1; GPx; KIAA0106; Liver 2D page spot 40; Ltw4; Lvtw 4; MGC46173; Non selenium glutathione peroxidase; NSGPx; ORF06; OTTHuMP00000032693; Peroxiredoxin6; PHGPx; Phospholipase A2 lysosomal; PLA2; Prdx5; PRX; Red blood cells page spot 12; Thiol specific antioxidant protein
 Product Gene Name   

PRDX6 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Sequence    MGSSHHHHHH SSGLVPRGSH MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP
Section Bar
Section Bar
 Purity/Purification    > 95% by SDS - PAGE
 Form/Format    Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 20% Glycerol
 Concentration    1 mg/ml (determined by Bradford assay) (lot specific)
Section Bar
 Antigen Species    Human
 Tag    His-tag
 Expression System    E Coli
 Bioactivity    Specific activity: approximately 95-120 pmole/min/ug. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX6 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute.
 Assay    1. Prepare a 50ul reaction mix into a suitable container :
The final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol.
2. Add 5 ul of recombinant PRDX6 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer.
3. Incubate at 25C for 2 minutes.
4. Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min.
5. Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction.
6. Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN)
7. Record the increase in A475nm.
Section Bar
 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of PRDX6 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for PRDX6 recombinant protein

   Peroxiredoxin 6, also known as PRDX6, is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell and can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Recombinant human peroxiredoxin 6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
 Product Categories/Family for PRDX6 recombinant protein    Redox Proteins
 Applications Tested/Suitable for PRDX6 recombinant protein   

SDS-PAGE

Section Bar
 SDS-PAGE of PRDX6 recombinant protein    PRDX6 recombinant protein SDS-PAGE image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PRDX6. It may not necessarily be applicable to this product.
 NCBI GI #    4758638
 NCBI GeneID    9588
 NCBI Accession #    NP_004896 [Other Products]
 NCBI GenBank Nucleotide #    NM_004905.2 [Other Products]
Section Bar
 UniProt Secondary Accession #    P32077; Q5TAH4; Q5ZEZ8; A8JZY7 [Other Products]
 UniProt Related Accession #    P30041 [Other Products]
 Molecular Weight    27.1 kDa (244aa), confirmed by MALDI-TOF. [Similar Products]
Table BarTOPTable Bar
 NCBI Official Full Name    peroxiredoxin-6
 NCBI Official Synonym Full Names    peroxiredoxin 6
 NCBI Official Symbol    PRDX6 [Similar Products]
 NCBI Official Synonym Symbols   
PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
[Similar Products]
 NCBI Protein Information    peroxiredoxin-6; 1-Cys PRX; 1-Cys peroxiredoxin; 24 kDa protein; acidic calcium-independent phospholipase A2; antioxidant protein 2; epididymis secretory sperm binding protein Li 128m; liver 2D page spot 40; non-selenium glutathione peroxidase; red blood cells page spot 12
 UniProt Protein Name    Peroxiredoxin-6
 UniProt Synonym Protein Names   
1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; Acidic calcium-independent phospholipase A2 (EC:3.1.1.-); aiPLA2; Antioxidant protein 2; Liver 2D page spot 40; Non-selenium glutathione peroxidase (EC:1.11.1.9); NSGPx; Red blood cells page spot 12
 Protein Family    Peroxiredoxin
 UniProt Gene Name    PRDX6 [Similar Products]
 UniProt Synonym Gene Names    AOP2; KIAA0106; 1-Cys PRX; aiPLA2; NSGPx [Similar Products]
 UniProt Entry Name    PRDX6_HUMAN
Section Bar
 NCBI Summary for PRDX6    The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for PRDX6    PRDX6: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Belongs to the AhpC/TSA family. Rehydrin subfamily.

Protein type: Oxidoreductase; Amino Acid Metabolism - phenylalanine; EC 1.11.1.15; Energy Metabolism - methane; Phospholipase; EC 1.11.1.9

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: extracellular space; membrane; lysosome; cytoplasm; cytoplasmic membrane-bound vesicle; cytosol

Molecular Function: phospholipase A2 activity; antioxidant activity; protein binding; peroxiredoxin activity; glutathione peroxidase activity

Biological Process: response to reactive oxygen species; phospholipid catabolic process; hydrogen peroxide catabolic process; response to oxidative stress
Table BarTOPTable Bar
 

Product References and Citations for PRDX6 recombinant protein

   Davison EJ., et al. (2009). Proteomics. 9(18):4284-97.
Wu J., et al. (2009). Chem Res Toxicol. 22(4):668-75.
Section Bar
 Research Articles on PRDX6    1. our findings indicate that PRDX6 promotes lung tumor growth via increased glutathione peroxidase and iPLA2 activities.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with PRDX6 recombinant proteinPathways associated with PRDX6 recombinant protein
 Reference Product  PubMed Publications
 CAT recombinant protein  >6 publications with PRDX6 and CAT
 TXN recombinant protein  >2 publications with PRDX6 and TXN
 TAT recombinant protein  >2 publications with PRDX6 and TAT
 PSIP1 recombinant protein  >1 publications with PRDX6 and PSIP1
 PAH recombinant protein  >1 publications with PRDX6 and PAH
 Products by Pathway  Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Detoxification Of Reactive Oxygen Species Pathway antibodies  Detoxification Of Reactive Oxygen Species Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
Diseases associated with PRDX6 recombinant proteinOrgans/Tissues associated with PRDX6 recombinant protein
 Disease Name  Pubmed Publications
 Death Antibodies  >23 publications with PRDX6 and Death
 Lung Diseases Antibodies  >17 publications with PRDX6 and Lung Diseases
 Nervous System Diseases Antibodies  >17 publications with PRDX6 and Nervous System Diseases
 Disease Models, Animal Antibodies  >17 publications with PRDX6 and Disease Models, Animal
 Inflammation Antibodies  >16 publications with PRDX6 and Inflammation
 Necrosis Antibodies  >9 publications with PRDX6 and Necrosis
 Cardiovascular Diseases Antibodies  >9 publications with PRDX6 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >8 publications with PRDX6 and Atherosclerosis
 Liver Diseases Antibodies  >7 publications with PRDX6 and Liver Diseases
 Lung Neoplasms Antibodies  >6 publications with PRDX6 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >47 publications with PRDX6 and Lung
 Blood Antibodies  >28 publications with PRDX6 and Blood
 Brain Antibodies  >27 publications with PRDX6 and Brain
 Liver Antibodies  >26 publications with PRDX6 and Liver
 Eye Antibodies  >16 publications with PRDX6 and Eye
 Vascular Antibodies  >16 publications with PRDX6 and Vascular
 Kidney Antibodies  >10 publications with PRDX6 and Kidney
 Muscle Antibodies  >9 publications with PRDX6 and Muscle
 Nerve Antibodies  >8 publications with PRDX6 and Nerve
 Skin Antibodies  >7 publications with PRDX6 and Skin
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions