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PPM1A recombinant protein :: Phosphatase 1A Alpha Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS142768 PPM1A recombinant protein
Unit / Price
0.01 mg  /  $140 +1 FREE 8GB USB
0.05 mg  /  $205 +1 FREE 8GB USB
1 mg  /  $1,800 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Phosphatase 1A Alpha (PPM1A), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human Protein Phosphatase 1A Alpha Isoform

 Product Synonym Names    PPM1A Human; Protein Phosphatase 1A Alpha Isoform Human Recombinant; Protein phosphatase 1A; EC 3.1.3.16; Protein phosphatase 2C isoform alpha; PP2C-alpha; IA; PPM1A; PP2CA; MGC9201
 Product Gene Name   

PPM1A recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW.
 OMIM    606108
 3D Structure    ModBase 3D Structure for P35813
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 Host    E Coli
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 Purity/Purification    Greater than 95.0% as determined by(a)Analysis by RP-HPLC. (b)Analysis by SDS-PAGE.
 Form/Format    The protein (1mg/ml) In phosphate-buffered saline (pH 7.4)
Sterile filtered colorless solution.
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 Unit Defenition    One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate per minute at pH 7.4 at 37 degree C using 10mM of substrate.
 Activity    8,000 U/mg.
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 Preparation and Storage    Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
 Other Notes    Small volumes of PPM1A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for PPM1A recombinant protein

   Description: Protein Phosphatase 1A Alpha Isoform Human Recombinant produced is a single, non-glycosylated polypeptide chain containing 382 amino acids and having a molecular mass of 46.6KDa (containing His tag, T7 gene 10 leader, XpressTM Epitope). The protein coding region of PP2Calpha (amino acids 1-382) was cloned into an E Coli expression vector. PPM1A was overexpressed in E Coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.

Introduction: Protein Phosphatase 2C alpha is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding two distinct isoforms have been described.Protein phosphatase 2C(PP2C?) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes.
 Product Categories/Family for PPM1A recombinant protein    PROTEIN KINASES; Enzymes; Phosphatase
NCBI/Uniprot data below describe general gene information for PPM1A. It may not necessarily be applicable to this product.
 NCBI GI #    10337595
 NCBI GeneID    5494
 NCBI Accession #    NP_066283.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021003.4 [Other Products]
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 UniProt Primary Accession #    P35813 [Other Products]
 UniProt Secondary Accession #    O75551; B5BU11; J3KNM0 [Other Products]
 UniProt Related Accession #    P35813 [Other Products]
 Molecular Weight    51,366 Da
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 NCBI Official Full Name    protein phosphatase 1A isoform 1
 NCBI Official Synonym Full Names    protein phosphatase, Mg2+/Mn2+ dependent, 1A
 NCBI Official Symbol    PPM1A [Similar Products]
 NCBI Official Synonym Symbols   
PP2CA; PP2Calpha; PP2C-ALPHA
[Similar Products]
 NCBI Protein Information    protein phosphatase 1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
 UniProt Protein Name    Protein phosphatase 1A
 UniProt Synonym Protein Names   
Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA
 Protein Family    Protein phosphatase
 UniProt Gene Name    PPM1A [Similar Products]
 UniProt Synonym Gene Names    PPPM1A; PP2C-alpha [Similar Products]
 UniProt Entry Name    PPM1A_HUMAN
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 NCBI Summary for PPM1A    The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PPM1A    PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: nucleoplasm; neuron projection; membrane; voltage-gated calcium channel complex; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity

Biological Process: N-terminal protein myristoylation; transcription initiation from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; Wnt receptor signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of I-kappaB kinase/NF-kappaB cascade; protein amino acid dephosphorylation; dephosphorylation; transforming growth factor beta receptor signaling pathway; insulin receptor signaling pathway; gene expression; cell cycle arrest; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of Wnt receptor signaling pathway
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 Research Articles on PPM1A    1. PPM1A is a RelA phosphatase that regulates NF-kappaB activity and that PPM1A has tumor suppressor-like activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PPM1A recombinant proteinPathways associated with PPM1A recombinant protein
 Reference Product  PubMed Publications
 SMAD2 recombinant protein  >13 publications with PPM1A and SMAD2
 SMAD5 recombinant protein  >3 publications with PPM1A and SMAD5
 CDK9 recombinant protein  >2 publications with PPM1A and CDK9
 SMAD4 recombinant protein  >1 publications with PPM1A and SMAD4
 Products by Pathway  Pathway Diagram
 BMP Receptor Signaling Pathway antibodies  BMP Receptor Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway antibodies  Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway Diagram
 Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway antibodies  Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 IGF1R Signaling Cascade Pathway antibodies  IGF1R Signaling Cascade Pathway Diagram
 IRS-mediated Signalling Pathway antibodies  IRS-mediated Signalling Pathway Diagram
 IRS-related Events Pathway antibodies  IRS-related Events Pathway Diagram
 IRS-related Events Triggered By IGF1R Pathway antibodies  IRS-related Events Triggered By IGF1R Pathway Diagram
Diseases associated with PPM1A recombinant proteinOrgans/Tissues associated with PPM1A recombinant protein
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >6 publications with PPM1A and Necrosis
 Fibrosis Antibodies  >4 publications with PPM1A and Fibrosis
 Disease Models, Animal Antibodies  >2 publications with PPM1A and Disease Models, Animal
 Kidney Diseases Antibodies  >2 publications with PPM1A and Kidney Diseases
 Depressive Disorder Antibodies  >1 publications with PPM1A and Depressive Disorder
 Neoplasm Metastasis Antibodies  >1 publications with PPM1A and Neoplasm Metastasis
 Carcinoma, Hepatocellular Antibodies  >1 publications with PPM1A and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >1 publications with PPM1A and Liver Neoplasms
 Inflammation Antibodies  >1 publications with PPM1A and Inflammation
 Liver Diseases Antibodies  >1 publications with PPM1A and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >6 publications with PPM1A and Bone
 Embryonic Tissue Antibodies  >4 publications with PPM1A and Embryonic Tissue
 Liver Antibodies  >3 publications with PPM1A and Liver
 Muscle Antibodies  >3 publications with PPM1A and Muscle
 Eye Antibodies  >2 publications with PPM1A and Eye
 Kidney Antibodies  >2 publications with PPM1A and Kidney
 Blood Antibodies  >2 publications with PPM1A and Blood
 Placenta Antibodies  >2 publications with PPM1A and Placenta
 Connective Tissue Antibodies  >2 publications with PPM1A and Connective Tissue
 Vascular Antibodies  >2 publications with PPM1A and Vascular
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