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cPLA2 recombinant protein :: Phospholipase A2, Cytosolic (cPLA2) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2011220 (3 units are in stock [0.1 mg] for immediate shipment)
Unit / Price
0.01 mg  /  $145 +1 FREE 8GB USB
0.05 mg  /  $280 +1 FREE 8GB USB
0.1 mg  /  $430 +1 FREE 8GB USB
0.2 mg  /  $530 +1 FREE 8GB USB
0.5 mg  /  $1,025 +1 FREE 8GB USB
1 mg  /  $1,525 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Phospholipase A2, Cytosolic (cPLA2), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Phospholipase A2, Cytosolic (cPLA2)

 Product Gene Name   

cPLA2 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 1; NC_000001.10 (186798032..186958113). Location: 1q25
 OMIM    600522
 3D Structure    ModBase 3D Structure for P47712
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 Host    E Coli
 Species Reactivity    Human
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 Purity/Purification    >92%
 Form/Format    PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
 Concentration    200ug/mL (lot specific)
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 Source    Prokaryotic expression
 Residues    Met1~Ser178
 Tags    N-terminal His-Tag
 Tissue Specificity    Blood
 Subcellular Location    Cytoplasm. Cytoplasmic vesicle
 Traits    Freeze-dried powder
    
 Predicted isoelectric point    5.9
 Predicted Molecular Mass    21.9kDa
 Accurate Molecular Mass    26kDa as determined by SDS-PAGE reducing conditions
 Phenomenon explanation    The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc
 Usage    Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex
    
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 Preparation and Storage    Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for two years.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of cPLA2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for cPLA2 recombinant protein   

SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

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 Sequence of cPLA2 recombinant protein    cPLA2 recombinant protein Sequence image
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 SDS-PAGE of cPLA2 recombinant protein    cPLA2 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for cPLA2. It may not necessarily be applicable to this product.
 NCBI GI #    23943920
 NCBI GeneID    5321
 NCBI Accession #    NP_077734.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_024420.2 [Other Products]
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 UniProt Primary Accession #    P47712 [Other Products]
 UniProt Secondary Accession #    Q29R80; B1AKG4 [Other Products]
 UniProt Related Accession #    P47712 [Other Products]
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 NCBI Official Full Name    cytosolic phospholipase A2
 NCBI Official Synonym Full Names    phospholipase A2, group IVA (cytosolic, calcium-dependent)
 NCBI Official Symbol    PLA2G4A [Similar Products]
 NCBI Official Synonym Symbols   
PLA2G4; cPLA2-alpha
[Similar Products]
 NCBI Protein Information    cytosolic phospholipase A2; cPLA2; lysophospholipase; phospholipase A2 group IVA; phosphatidylcholine 2-acylhydrolase; calcium-dependent phospholipid-binding protein
 UniProt Protein Name    Cytosolic phospholipase A2
 UniProt Synonym Protein Names   
Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase
 UniProt Gene Name    PLA2G4A [Similar Products]
 UniProt Synonym Gene Names    CPLA2; PLA2G4; cPLA2 [Similar Products]
 UniProt Entry Name    PA24A_HUMAN
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 NCBI Summary for cPLA2    This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
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 UniProt Comments for cPLA2    cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A.

Protein type: EC 3.1.1.5; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4; Lipid Metabolism - linoleic acid; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - arachidonic acid; Phospholipase

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cytoplasm; mitochondrial inner membrane; cytoplasmic membrane-bound vesicle; cytosol

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; calcium ion binding; lysophospholipase activity

Biological Process: icosanoid biosynthetic process; platelet activation; platelet activating factor biosynthetic process; phospholipid catabolic process; phospholipid metabolic process; icosanoid metabolic process; glycerophospholipid biosynthetic process; arachidonic acid metabolic process; phosphatidic acid biosynthetic process; arachidonic acid secretion; blood coagulation; regulation of cell proliferation
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 Research Articles on cPLA2    1. LacCer is a direct activator of cPLA2alpha.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with cPLA2 recombinant proteinPathways associated with cPLA2 recombinant protein
 Reference Product  PubMed Publications
 PTGS2 recombinant protein  >283 publications with cPLA2 and PTGS2
 PLA2G6 recombinant protein  >102 publications with cPLA2 and PLA2G6
 PTGS1 recombinant protein  >89 publications with cPLA2 and PTGS1
 ALOX5 recombinant protein  >56 publications with cPLA2 and ALOX5
 SYK recombinant protein  >7 publications with cPLA2 and SYK
 PLD2 recombinant protein  >4 publications with cPLA2 and PLD2
 KAT5 recombinant protein  >2 publications with cPLA2 and KAT5
 MKNK1 recombinant protein  >1 publications with cPLA2 and MKNK1
 Products by Pathway  Pathway Diagram
 ADP Signalling Through P2Y Purinoceptor 1 Pathway antibodies  ADP Signalling Through P2Y Purinoceptor 1 Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Acyl Chain Remodeling Of CL Pathway antibodies  Acyl Chain Remodeling Of CL Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Acyl Chain Remodelling Of PE Pathway antibodies  Acyl Chain Remodelling Of PE Pathway Diagram
 Acyl Chain Remodelling Of PG Pathway antibodies  Acyl Chain Remodelling Of PG Pathway Diagram
 Acyl Chain Remodelling Of PI Pathway antibodies  Acyl Chain Remodelling Of PI Pathway Diagram
 Acyl Chain Remodelling Of PS Pathway antibodies  Acyl Chain Remodelling Of PS Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
Diseases associated with cPLA2 recombinant proteinOrgans/Tissues associated with cPLA2 recombinant protein
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >288 publications with cPLA2 and Inflammation
 Neoplasms Antibodies  >216 publications with cPLA2 and Neoplasms
 Necrosis Antibodies  >163 publications with cPLA2 and Necrosis
 Nervous System Diseases Antibodies  >135 publications with cPLA2 and Nervous System Diseases
 Disease Models, Animal Antibodies  >112 publications with cPLA2 and Disease Models, Animal
 Brain Diseases Antibodies  >110 publications with cPLA2 and Brain Diseases
 Cardiovascular Diseases Antibodies  >105 publications with cPLA2 and Cardiovascular Diseases
 Asthma Antibodies  >38 publications with cPLA2 and Asthma
 Edema Antibodies  >34 publications with cPLA2 and Edema
 Heart Diseases Antibodies  >33 publications with cPLA2 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >269 publications with cPLA2 and Brain
 Vascular Antibodies  >209 publications with cPLA2 and Vascular
 Lung Antibodies  >184 publications with cPLA2 and Lung
 Kidney Antibodies  >144 publications with cPLA2 and Kidney
 Embryonic Tissue Antibodies  >62 publications with cPLA2 and Embryonic Tissue
 Ovary Antibodies  >46 publications with cPLA2 and Ovary
 Eye Antibodies  >43 publications with cPLA2 and Eye
 Uterus Antibodies  >39 publications with cPLA2 and Uterus
 Intestine Antibodies  >35 publications with cPLA2 and Intestine
 Skin Antibodies  >34 publications with cPLA2 and Skin
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