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lasA recombinant protein :: Protease lasA (lasA) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1185217
SDS-PAGE
Unit / Price
0.01 mg (E-Coli)  /  $230 +1 FREE 8GB USB
0.05 mg (E-Coli)  /  $310 +1 FREE 8GB USB
0.1 mg (E-Coli)  /  $515 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $735 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $830 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $885 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $995 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $1,090 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $1,115 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,115 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,120 +1 FREE 8GB USB
0.5 mg (Baculovirus)  /  $1,610 +2 FREE 8GB USB
1 mg (Yeast)  /  $1,690 +2 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $1,710 +2 FREE 8GB USB
1 mg (E-Coli)  /  $1,725 +2 FREE 8GB USB
1 mg (Baculovirus)  /  $2,155 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Protease lasA (lasA), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Pseudomonas aeruginosa Protease lasA (lasA)

 Product Synonym Names    Protease lasA; EC=3.4.24.-; Staphylolytic protease
 Product Gene Name   

lasA recombinant protein

[Similar Products]
 Product Synonym Gene Name    lasA; PA1871 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 MBS1185217 COA    COA PDF
 Sequence Positions    237-418. Full length of the mature protein.
 Sequence    APPSNLMQLP WRQGYSWQPN GAHSNTGSGY PYSSFDASYD WPRWGSATYS VVAAHAGTVR VLSRCQVRVT HPSGWATNYY HMDQIQVSNG QQVSADTKLG VYAGNINTAL CEGGSSTGPH LHFSLLYNGA FVSLQGASFG PYRINVGTSN YDNDCRRYYF YNQSAGTTHC AFRPLYNPGL AL
 3D Structure    ModBase 3D Structure for P14789
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    >=90% (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Species    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
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 Preparation and Storage    Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of lasA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for lasA recombinant protein

   Involved in proteolysis and elastolysis (degradation of the host protein elastin). Has staphylolytic activity (degrades pentaglycine cross-links in cell wall peptidogylcan), preferring Gly-Gly-|-X substrates where X is Ala or Gly (PubMed:11179971). Enhances the elastolytic but not proteolytic activity of elastase (lasB) and elastolytic activity of other proteases (PubMed:2110137). Degradation of host elastin is likely to contribute to the pathogenicity of P.aeruginosa. While either His-317 or His-356 can abstract a proton in the hydrolysis reaction, the same residue performs both functions in a given catalytic cycle, with the other stabilizing the catalytic intermediate (PubMed:20026068).
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 SDS-PAGE of lasA recombinant protein    lasA recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for lasA. It may not necessarily be applicable to this product.
 NCBI GI #    15597068
 NCBI GeneID    878260
 NCBI Accession #    NP_250562.1 [Other Products]
 NCBI GenBank Nucleotide #    NC_002516.2 [Other Products]
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 UniProt Primary Accession #    P14789 [Other Products]
 UniProt Secondary Accession #    P72165; Q9I2M5 [Other Products]
 UniProt Related Accession #    P14789 [Other Products]
 Molecular Weight    35kD
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 NCBI Official Full Name    LasA protease
 NCBI Official Symbol    lasA [Similar Products]
 NCBI Protein Information    LasA protease
 UniProt Protein Name    Protease LasA
 UniProt Synonym Protein Names   
Staphylolytic protease
 Protein Family    Protease
 UniProt Gene Name    lasA [Similar Products]
 UniProt Synonym Gene Names    PA1871 [Similar Products]
 UniProt Entry Name    LASA_PSEAE
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 UniProt Comments for lasA    Involved in proteolysis and elastolysis (degradation of the host protein elastin). Has staphylolytic activity (degrades pentaglycine cross-links in cell wall peptidogylcan), preferring Gly-Gly-|-X substrates where X is Ala or Gly (PubMed:11179971). Enhances the elastolytic but not proteolytic activity of elastase (lasB) and elastolytic activity of other proteases (PubMed:2110137). Degradation of host elastin is likely to contribute to the pathogenicity of P.aeruginosa. While either His-317 or His-356 can abstract a proton in the hydrolysis reaction, the same residue performs both functions in a given catalytic cycle, with the other stabilizing the catalytic intermediate (PubMed:20026068).
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 Research Articles on lasA    1. crystal structures of active LasA as a complex with tartrate and in the uncomplexed form. While the overall fold resembles that of the other M23 family members, the LasA active site is less constricted and utilizes a different set of metal ligands.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with lasA recombinant protein
 Reference Product  PubMed Publications
 apr recombinant protein  >75 publications with lasA and apr
 lasB recombinant protein  >36 publications with lasA and lasB
 lasR recombinant protein  >21 publications with lasA and lasR
 rhlR recombinant protein  >12 publications with lasA and rhlR
 lasI recombinant protein  >11 publications with lasA and lasI
 rhlI recombinant protein  >8 publications with lasA and rhlI
 exoS recombinant protein  >4 publications with lasA and exoS
 ampR recombinant protein  >2 publications with lasA and ampR
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