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PTPN1 recombinant protein :: Protein Tyrosine Phosphatase 1B Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS636074
SDS-PAGE
Unit / Price
0.25 mg  /  $445 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Protein Tyrosine Phosphatase 1B (PTPN1), Recombinant Protein

★Popular Item★
 Also Known As   

Protein Tyrosine Phosphatase 1B, Recombinant, Human (PTP1B)

 Product Gene Name   

PTPN1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MEMEKEFEQ I/ DKSGSWAAIY / QDIRHEASDF / PCRVAKLPKN / KNRNRYRDVS / PFDHSRIKLH / QEDNDYIN AS/ LIKMEEAQRS / YILTQGPLPN / TCGHFWEMVW / EQKSRGVVML / NRVMEKGSLK / CAQYWPQK EE/ KEMIFEDTNL / KLTLISEDIK / SYYTVRQLEL / ENLTTQETRE / ILHFHYTTWP / DFGVPESP AS/ FLNFLFKVRE / SGSLSPEHGP / VVVHCSAGIG / RSGTFCLADT / CLLLMDKRKD / PSSVDIKK VL/ LEMRKFRMGL / IQTADQLRFS / YLAVIEGAKF / IMGDSSVQDQ / WKELSHEDLE / PPPEHIPP PP/ RPPKRILEPH / N
 Chromosome Location    Chromosome: 20; NC_000020.10 (49126858..49201299). Location: 20q13.1-q13.2
 OMIM    176885
 3D Structure    ModBase 3D Structure for P18031
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 Host    E Coli
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 Purity/Purification    Highly Purified
>95% by SDS PAGE; Purified by using conventional chromatography techniques.
 Form/Format    Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mM DTT, 20% glycerol.
 Concentration    1mg/ml (lot specific)
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 Specific Activity    >10,000units/mg
 Unit Definition    One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate (p-NPP) per minute at pH 7.4, 37 degree C using 10mM of substrate.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C . Aliquots are stable for 6 months at -20 degree C . For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of PTPN1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for PTPN1 recombinant protein

   The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector.
 Product Categories/Family for PTPN1 recombinant protein    Molecular Biology; MB-Enzymes, Phosphatase
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 SDS-PAGE of PTPN1 recombinant protein    SDS-PAGE analysis of PTP 1 B (3ug protein; 12% gel)
PTPN1 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for PTPN1. It may not necessarily be applicable to this product.
 NCBI GI #    13919645
 NCBI GeneID    5770
 NCBI Accession #    AAK31734.1 [Other Products]
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 UniProt Primary Accession #    P18031 [Other Products]
 UniProt Secondary Accession #    Q5TGD8; Q9BQV9; Q9NQQ4 [Other Products]
 UniProt Related Accession #    P18031 [Other Products]
 Molecular Weight    37,312 [Similar Products]
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 NCBI Official Full Name    protein tyrosine phosphatase 1B
 NCBI Official Synonym Full Names    protein tyrosine phosphatase, non-receptor type 1
 NCBI Official Symbol    PTPN1 [Similar Products]
 NCBI Official Synonym Symbols   
PTP1B
[Similar Products]
 NCBI Protein Information    tyrosine-protein phosphatase non-receptor type 1; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, placental
 UniProt Protein Name    Tyrosine-protein phosphatase non-receptor type 1
 UniProt Synonym Protein Names   
Protein-tyrosine phosphatase 1B
 Protein Family    Tyrosine-protein phosphatase non-receptor
 UniProt Gene Name    PTPN1 [Similar Products]
 UniProt Synonym Gene Names    PTP1B; PTP-1B [Similar Products]
 UniProt Entry Name    PTN1_HUMAN
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 NCBI Summary for PTPN1    The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]
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 UniProt Comments for PTPN1    PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes.

Protein type: Motility/polarity/chemotaxis; Phosphatase; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 20q13.1-q13.2

Cellular Component: endoplasmic reticulum; early endosome; plasma membrane; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; enzyme binding; ephrin receptor binding; zinc ion binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding; insulin receptor binding

Biological Process: platelet activation; unfolded protein response, activation of signaling protein activity; negative regulation of vascular endothelial growth factor receptor signaling pathway; unfolded protein response; cytokine and chemokine mediated signaling pathway; regulation of endocytosis; actin cytoskeleton reorganization; insulin receptor signaling pathway; negative regulation of insulin receptor signaling pathway; regulation of signal transduction; blood coagulation

Disease: Diabetes Mellitus, Noninsulin-dependent
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 Research Articles on PTPN1    1. Conformational stability of WPD loop (residues Tyr176-Ser187), and possibly catalytic activity of PTP1B, is significantly affected by the protonation state of Asp181 and position of active site water molecules.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PTPN1 recombinant proteinPathways associated with PTPN1 recombinant protein
 Reference Product  PubMed Publications
 SRC recombinant protein  >55 publications with PTPN1 and SRC
 IRS1 recombinant protein  >29 publications with PTPN1 and IRS1
 JAK2 recombinant protein  >21 publications with PTPN1 and JAK2
 EGFR recombinant protein  >18 publications with PTPN1 and EGFR
 AKT1 recombinant protein  >8 publications with PTPN1 and AKT1
 IGF1R recombinant protein  >7 publications with PTPN1 and IGF1R
 BCAR1 recombinant protein  >4 publications with PTPN1 and BCAR1
 INSR recombinant protein  >4 publications with PTPN1 and INSR
 TRPV6 recombinant protein  >2 publications with PTPN1 and TRPV6
 GRB2 recombinant protein  >2 publications with PTPN1 and GRB2
 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 EGF Receptor (ErbB1) Signaling Pathway antibodies  EGF Receptor (ErbB1) Signaling Pathway Diagram
 Growth Hormone Receptor Signaling Pathway antibodies  Growth Hormone Receptor Signaling Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IGF1 Pathway antibodies  IGF1 Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Insulin Pathway antibodies  Insulin Pathway Diagram
Diseases associated with PTPN1 recombinant proteinOrgans/Tissues associated with PTPN1 recombinant protein
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >134 publications with PTPN1 and Insulin Resistance
 Diabetes Mellitus, Type 2 Antibodies  >72 publications with PTPN1 and Diabetes Mellitus, Type 2
 Neoplasms Antibodies  >70 publications with PTPN1 and Neoplasms
 Cardiovascular Diseases Antibodies  >35 publications with PTPN1 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >30 publications with PTPN1 and Disease Models, Animal
 Hyperglycemia Antibodies  >27 publications with PTPN1 and Hyperglycemia
 Necrosis Antibodies  >21 publications with PTPN1 and Necrosis
 Inflammation Antibodies  >20 publications with PTPN1 and Inflammation
 Weight Gain Antibodies  >19 publications with PTPN1 and Weight Gain
 Liver Diseases Antibodies  >16 publications with PTPN1 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >108 publications with PTPN1 and Blood
 Muscle Antibodies  >74 publications with PTPN1 and Muscle
 Liver Antibodies  >73 publications with PTPN1 and Liver
 Brain Antibodies  >37 publications with PTPN1 and Brain
 Connective Tissue Antibodies  >29 publications with PTPN1 and Connective Tissue
 Heart Antibodies  >23 publications with PTPN1 and Heart
 Vascular Antibodies  >20 publications with PTPN1 and Vascular
 Kidney Antibodies  >17 publications with PTPN1 and Kidney
 Pancreas Antibodies  >12 publications with PTPN1 and Pancreas
 Bone Antibodies  >10 publications with PTPN1 and Bone
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