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Pdk4 recombinant protein :: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial (Pdk4), partial Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1216721
SDS-Page
Unit / Price
0.5 mg (E-Coli)  /  $1,015 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $1,015 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,240 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $1,240 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,510 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,510 +1 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $1,970 +2 FREE 8GB USB
1 mg (Yeast)  /  $1,970 +2 FREE 8GB USB
0.5 mg (Baculovirus)  /  $2,005 +2 FREE 8GB USB
1 mg (Baculovirus)  /  $2,970 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial (Pdk4), partial, Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Mouse [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial (Pdk4), partial

 Product Synonym Names    [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; EC=2.7.11.2; Pyruvate dehydrogenase kinase isoform 4
 Product Gene Name   

Pdk4 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 MBS1216721 COA    COA PDF
 Sequence Positions    138-368aa; Partial
 Sequence    ILEYKDTCTV DPVTNQNLQY FLDRFYMNRI STRMLMNQHI LIFSDSKTGN PSHIGSIDPN CDVVAVVQDA FECAKMLCDQ YYLTSPELNL TQVNGKFPGQ PIHIVYVPSH LHHMLFELFK NAMRATVEHQ ENRPSLTPVE ATVVLGKEDL TIKISDRGGG VPLRITDRLF SYTYSTAPTP VMDNSRNAPL AGFGYGLPIS RLYAKYFQGD LNLYSMSGYG TDAIIYLKAL S
 3D Structure    ModBase 3D Structure for O70571
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    >=90% (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Species    Mus musculus (Mouse)
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 Preparation and Storage    Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of Pdk4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Pdk4 recombinant protein

   Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism
 Product Categories/Family for Pdk4 recombinant protein    Metabolism
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 SDS-Page of Pdk4 recombinant protein    Pdk4 recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for Pdk4. It may not necessarily be applicable to this product.
 NCBI GI #    7305375
 NCBI GeneID    27273
 NCBI Accession #    NP_038771.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_013743.2 [Other Products]
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 UniProt Primary Accession #    O70571 [Other Products]
 UniProt Related Accession #    O70571 [Other Products]
 Molecular Weight    46,596 Da
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 NCBI Official Full Name    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
 NCBI Official Synonym Full Names    pyruvate dehydrogenase kinase, isoenzyme 4
 NCBI Official Symbol    Pdk4 [Similar Products]
 NCBI Official Synonym Symbols   
AV005916
[Similar Products]
 NCBI Protein Information    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; pyruvate dehydrogenase kinase 4; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
 UniProt Protein Name    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
 UniProt Synonym Protein Names   
Pyruvate dehydrogenase kinase isoform 4
 UniProt Gene Name    Pdk4 [Similar Products]
 UniProt Entry Name    PDK4_MOUSE
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 UniProt Comments for Pdk4    PDHK4: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK4 is abundant in heart, skeletal muscle, kidney, and pancreatic islets. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: EC 2.7.11.2; Protein kinase, atypical; Kinase, protein; Mitochondrial; ATYPICAL group; PDHK family

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: transferase activity; nucleotide binding; protein serine/threonine/tyrosine kinase activity; kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity

Biological Process: acetyl-CoA biosynthetic process from pyruvate; regulation of bone resorption; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; cellular response to starvation; glucose homeostasis; protein amino acid phosphorylation; response to starvation; regulation of fatty acid biosynthetic process; carbohydrate metabolic process; regulation of fatty acid oxidation; insulin receptor signaling pathway; regulation of pH; phosphorylation
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 Research Articles on Pdk4    1. Diminished insulin signaling evident at 1 wk on the high fat diet did not occur in pyruvate dehydrogenase kinase 4 knockout mice.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Pdk4 recombinant proteinPathways associated with Pdk4 recombinant protein
 Reference Product  PubMed Publications
 Foxo1 recombinant protein  >23 publications with Pdk4 and Foxo1
 Cpt1b recombinant protein  >7 publications with Pdk4 and Cpt1b
 Foxo3 recombinant protein  >3 publications with Pdk4 and Foxo3
 Prl recombinant protein  >2 publications with Pdk4 and Prl
 Pdha1 recombinant protein  >1 publications with Pdk4 and Pdha1
 Pdhx recombinant protein  >1 publications with Pdk4 and Pdhx
 Products by Pathway  Pathway Diagram
 Amino Acid Metabolism Pathway antibodies  Amino Acid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Pyruvate Metabolism Pathway antibodies  Pyruvate Metabolism Pathway Diagram
 Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway antibodies  Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway Diagram
 Regulation Of Pyruvate Dehydrogenase (PDH) Complex Pathway antibodies  Regulation Of Pyruvate Dehydrogenase (PDH) Complex Pathway Diagram
 TCA Cycle Pathway antibodies  TCA Cycle Pathway Diagram
 The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway antibodies  The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway Diagram
Diseases associated with Pdk4 recombinant proteinOrgans/Tissues associated with Pdk4 recombinant protein
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >51 publications with Pdk4 and Insulin Resistance
 Fatty Liver Antibodies  >39 publications with Pdk4 and Fatty Liver
 Inflammation Antibodies  >22 publications with Pdk4 and Inflammation
 Heart Diseases Antibodies  >19 publications with Pdk4 and Heart Diseases
 Cardiovascular Diseases Antibodies  >19 publications with Pdk4 and Cardiovascular Diseases
 Hypertrophy Antibodies  >14 publications with Pdk4 and Hypertrophy
 Liver Diseases Antibodies  >13 publications with Pdk4 and Liver Diseases
 Necrosis Antibodies  >12 publications with Pdk4 and Necrosis
 Hyperglycemia Antibodies  >11 publications with Pdk4 and Hyperglycemia
 Weight Loss Antibodies  >10 publications with Pdk4 and Weight Loss
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >140 publications with Pdk4 and Muscle
 Heart Antibodies  >47 publications with Pdk4 and Heart
 Brain Antibodies  >12 publications with Pdk4 and Brain
 Kidney Antibodies  >11 publications with Pdk4 and Kidney
 Pancreas Antibodies  >11 publications with Pdk4 and Pancreas
 Bone Antibodies  >3 publications with Pdk4 and Bone
 Skin Antibodies  >2 publications with Pdk4 and Skin
 Bone Marrow Antibodies  >2 publications with Pdk4 and Bone Marrow
 Intestine Antibodies  >2 publications with Pdk4 and Intestine
 Lung Antibodies  >2 publications with Pdk4 and Lung
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