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RIPK3 recombinant protein :: Receptor Interacting Serine Threonine Kinase 3 (RIPK3) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2011887 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $145 +1 FREE 8GB USB
0.05 mg  /  $280 +1 FREE 8GB USB
0.1 mg  /  $435 +1 FREE 8GB USB
0.2 mg  /  $535 +1 FREE 8GB USB
0.5 mg  /  $1,030 +1 FREE 8GB USB
1 mg  /  $1,535 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Receptor Interacting Serine Threonine Kinase 3 (RIPK3), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Receptor Interacting Serine Threonine Kinase 3 (RIPK3)

 Product Gene Name   

RIPK3 recombinant protein

[Similar Products]
 Matching Pairs    Antibody: Receptor Interacting Serine Threonine Kinase 3 (RIPK3) (MBS2003562)
Antigen: Receptor Interacting Serine Threonine Kinase 3 (RIPK3) (MBS2011887)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- V KIVNSKKISR EVKAMVNLRH ENVLLLLGVT ENLEWDYVYG PALVTGFMEN GSLSGLLQPS CPRPWPLLCR LLEEVVLGMC YLHSLNPSLL HRDLKPSNVL LDLELHAKLA DFGLSTFQGG SQSGSGSGSR DSGGTLAYLA PELLDNDGKA SKASDVYSFG VLVWTVLAGR EAEVVDKTSL IRGAVCNRQR RPPLTELPPD SPETPGLEGL KELMTHCWSS EP
 Chromosome Location    Chromosome: 15; NC_005114.3 (38359304..38368247, complement). Location: 15p13
 3D Structure    ModBase 3D Structure for Q9Z2P5
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
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 Organism    Rattus norvegicus (Rat)
 Expression System    Prokaryotic expression
 Residues    Val50~Pro272 (Accession # Q9Z2P5) with two N-terminal Tags, His-tag and T7-tag
 Usage    Reconstitute in ddH2O.
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
 Predicted isoelectric point    6.3
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of RIPK3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for RIPK3 recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for RIPK3 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of RIPK3 recombinant protein    RIPK3 recombinant protein SDS-PAGE image
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 SDS-PAGE of RIPK3 recombinant protein    [ DESCRIPTION]
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa.
Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SDS-polyacrylamide gel.
[ LOADING VOLUMES]
3~5uL per well for mini gel; 7uL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by Dn oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
RIPK3 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for RIPK3. It may not necessarily be applicable to this product.
 NCBI GI #    21326477
 NCBI GeneID    246240
 NCBI Accession #    NP_647558.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_139342.1 [Other Products]
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 UniProt Primary Accession #    Q9Z2P5 [Other Products]
 UniProt Related Accession #    Q9Z2P5 [Other Products]
 Molecular Weight    28.1kDa
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 NCBI Official Full Name    receptor-interacting serine/threonine-protein kinase 3
 NCBI Official Synonym Full Names    receptor-interacting serine-threonine kinase 3
 NCBI Official Symbol    Ripk3 [Similar Products]
 NCBI Official Synonym Symbols   
Hcyp2
[Similar Products]
 NCBI Protein Information    receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3; receptor-interacting protein 3; homocysteine respondent protein HCYP2
 UniProt Protein Name    Receptor-interacting serine/threonine-protein kinase 3
 UniProt Synonym Protein Names   
Homocysteine respondent protein HCYP2; RIP-like protein kinase 3; Receptor-interacting protein 3
 Protein Family    Receptor-interacting serine/threonine-protein kinase
 UniProt Gene Name    Ripk3 [Similar Products]
 UniProt Synonym Gene Names    Rip3; RIP-3 [Similar Products]
 UniProt Entry Name    RIPK3_RAT
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 NCBI Summary for RIPK3    a putative homocysteine respondent protein [RGD, Feb 2006]
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 UniProt Comments for RIPK3    RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; RIPK family

Cellular Component: mitochondrion; plasma membrane; intracellular; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; identical protein binding; protein complex binding; ATP binding; protein kinase activity

Biological Process: spleen development; regulation of activated T cell proliferation; I-kappaB kinase/NF-kappaB cascade; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; T cell differentiation in the thymus; positive regulation of transferase activity; protein amino acid phosphorylation; lymph node development; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; activation of protein kinase activity; T cell homeostasis; positive regulation of ligase activity; protein homooligomerization; positive regulation of oxidoreductase activity
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 Research Articles on RIPK3    1. Receptor interacting protein 3 suppresses vascular smooth muscle cell growth by inhibition of the phosphoinositide 3-kinase-Akt axis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with RIPK3 recombinant proteinDiseases associated with RIPK3 recombinant protein
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 IKK Complex Recruitment Mediated By RIP1 Pathway antibodies  IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 MyD88-independent Cascade Pathway antibodies  MyD88-independent Cascade Pathway Diagram
 RIP-mediated NFkB Activation Via ZBP1 Pathway antibodies  RIP-mediated NFkB Activation Via ZBP1 Pathway Diagram
 TNF Signaling Pathway antibodies  TNF Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >105 publications with RIPK3 and Necrosis
 Inflammation Antibodies  >35 publications with RIPK3 and Inflammation
 Cardiovascular Diseases Antibodies  >8 publications with RIPK3 and Cardiovascular Diseases
 Liver Diseases Antibodies  >6 publications with RIPK3 and Liver Diseases
 Carcinoma Antibodies  >5 publications with RIPK3 and Carcinoma
 Drug-Induced Liver Injury Antibodies  >4 publications with RIPK3 and Drug-Induced Liver Injury
 Weight Loss Antibodies  >2 publications with RIPK3 and Weight Loss
 Liver Neoplasms Antibodies  >2 publications with RIPK3 and Liver Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with RIPK3 and Cell Transformation, Neoplastic
 Drug-Related Side Effects and Adverse Reactions Antibodies  >2 publications with RIPK3 and Drug-Related Side Effects and Adverse Reactions
Organs/Tissues associated with RIPK3 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with RIPK3 and Brain
 Embryonic Tissue Antibodies  >5 publications with RIPK3 and Embryonic Tissue
 Heart Antibodies  >3 publications with RIPK3 and Heart
 Muscle Antibodies  >3 publications with RIPK3 and Muscle
 Small Intestine Antibodies  >1 publications with RIPK3 and Small Intestine
 Spleen Antibodies  >1 publications with RIPK3 and Spleen
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